PINK1/Parkin通路介导的白藜芦醇对H2O2处理的骨关节炎成纤维样滑膜细胞的影响

doi:10.3969/j.issn.1006-5725.2026.03.002

Abstract

Abstract:

Objective To investigate the effect of resveratrol （Res） on fibroblast-like synovial cells of osteoarthritis treated with H₂O₂， and to clarify the regulatory mechanism relationship of the PINK1/Parkin mitochondrial autophagy signaling pathway in the above effects. Methods Fibroblast-like synovial cells （FLSs） were extracted from the synovial membranes of patients undergoing osteoarthritis surgery and divided into the control group and the experimental group. The experimental group was treated with H₂O₂ （5 μmol/L） and then treated with different concentrations of Res （0， 10， 20， 40， 60， 80， 100 μmol/L）. Cell viability assay （CCK-8） was used to detect the inhibition rate of cell proliferation. Cell reactive oxygen species were detected by DCFH-DA fluorescent probe. Mitochondrial reactive oxygen species were detected by MitoSOX Red fluorescent probe. Cell cycle was detected by flow cytometry. The expressions of proteins such as NLRP3， TNF-α， IL-1β， SOD2， Bax， Bcl-2， BNIP3， LC3B， p62， PINK1 and Parkin were detected by Western blot and immunofluorescence experiments. The role of the PINK1/Parkin mitochondrial autophagy signaling pathway in Res' inhibition of abnormal proliferation， inflammation and oxidative stress in FLSs was further verified using PINK1 and Parkin interfering RNA. Results Res significantly inhibited the enhancement of FLSs activity induced by H₂O₂， and reduced the abnormal proliferation， inflammation and oxidative stress levels of FLSs； Res inhibited the expressions of NLRP3， TNF-α， Bcl-2 and p62 proteins， while promoting the expressions of SOD2， Bax， BNIP3， LC3B， PINK1 and Parkin proteins. H₂O₂ inhibited the PINK1/Parkin signaling pathway， while Res activated the PINK1/Parkin pathway. The use of PINK1 and Parkin interfering RNA exacerbated cellular inflammation and oxidative stress. Conclusion Res activates the PINK1/Parkin mitochondrial autophagy signaling pathway by increasing the expression of PINK1 and Parkin proteins， alleviating abnormal proliferation， inflammation and oxidative stress in fibroblast-like synovial cells of osteoarthritis induced by H₂O₂.

Key words: resveratrol, osteoarthritis, fibroblast-like synovial cells, PINK1/Parkin signaling pathway, inflammation, oxidative stress

CLC Number:

R361.1

Lixia ZHANG,Xuefei FAN,Suhuan CHEN,Mengyan ZHANG,Yubao SHAO,Jian ZHOU,Xiaoyu CHEN. Effect of resveratrol mediated by the PINK1/Parkin pathway on fibroblast-like synovial cells of osteoarthritis treated with H₂O₂[J]. The Journal of Practical Medicine, 2026, 42(3): 363-370.

Figures/Tables 13

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References 21

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组别	例数	ROS	NLRP3	TNF-α	SOD2	IL-1β	PINK1	Parkin
FLSs组	10	8.73 ± 0.22	0.13 ± 0.05	0.42 ± 0.33	1.47 ± 0.30	0.38 ± 0.09	0.85 ± 0.45	1.18 ± 0.52
OA-FLSs组	10	23.65 ± 0.43	0.28 ± 0.12	1.87 ± 0.65	0.76 ± 0.67	0.96 ± 0.22	0.37 ± 0.30	0.35 ± 0.28
t值		27.65	9.95	18.81	12.18	11.88	10.56	15.61
P值		0.01	0.05	0.01	0.05	0.05	0.05	0.05

组别	例数	CCK-8 assay（OD 450）
对照组	3	1.05 ± 0.22
H₂O₂（2.5 μmol/L）	3	1.15 ± 0.48
H₂O₂（5 μmol/L）	3	1.25 ± 0.52^?
H₂O₂（10 μmol/L）	3	0.80 ± 0.77^?
H₂O₂（20 μmol/L）	3	0.76 ± 0.29^?
H₂O₂（40 μmol/L）	3	0.64 ± 0.40^??
H₂O₂（80 μmol/L）	3	0.48 ± 0.12^??
H₂O₂（100 μmol/L）	3	0.25 ± 0.38^??
F值		18.81
P值		0.05

组别	例数	CCK-8 assay（OD 450）
对照组	3	1.02 ± 0.12
Res（5 μmol/L）组	3	1.28 ± 0.29^#
Res（10 μmol/L）组	3	0.92 ± 0.64^?
Res（20 μmol/L）组	3	0.81 ± 0.50^?
Res（40 μmol/L）组	3	0.66 ± 0.12^?
Res（60 μmol/L）组	3	0.46 ± 0.33^??
Res（80 μmol/L）组	3	0.38 ± 0.11^??
Res（100 μmol/L）组	3	0.24 ± 0.16^??
F值		19.80
P值		0.05

组别	例数	NLRP3	TNF-α	SOD2	IL-1β	mtROS
对照组	3	0.27 ± 0.17	0.82 ± 0.22	0.79 ± 0.26	0.47 ± 0.10	8.23 ± 2.76
H₂O₂组	3	0.53 ± 0.16^#	1.28 ± 0.35^#	0.48 ± 0.20^#	0.79 ± 0.19^#	23.79 ± 4.22^##
Res组	3	0.29 ± 0.23^?	0.80 ± 0.30^?	0.78 ± 0.17^?	0.52 ± 0.23^?	8.85 ± 3.98^??
F值		16.09	21.11	15.58	17.98	25.61
P值		0.05	0.05	0.05	0.05	0.01

组别	例数	Bax	Bcl-2	Cells in S-Phase/%
对照组	3	0.59 ± 0.16	0.62 ± 0.11	22.55 ± 4.70
H₂O₂组	3	0.23 ± 0.19^#	1.27 ± 0.25^#	35.49 ± 5.02^#
Res组	3	0.55 ± 0.24^?	0.73 ± 0.29^?	23.85 ± 6.23^?
F值		32.90	45.50	25.61
P值		0.05	0.05	0.05

Effect of resveratrol mediated by the PINK1/Parkin pathway on fibroblast-like synovial cells of osteoarthritis treated with H₂O₂

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组别	例数	p62	BNIP3	LC3B-Ⅱ	PINK1	Parkin
对照组	3	0.26 ± 0.15	0.92 ± 0.34	0.78 ± 0.25	0.67 ± 0.17	1.02 ± 0.33
H₂O₂组	3	0.57 ± 0.31^#	0.53 ± 0.15^#	0.26 ± 0.12^#	0.21 ± 0.19^#	0.31 ± 0.17^#
Res组	3	0.29 ± 0.20^?	0.82 ± 0.22^?	0.75 ± 0.21^?	0.63 ± 0.20^?	0.88 ± 0.28^?
F值		18.19	23.00	17.61	26.50	27.26
P值		0.05	0.05	0.05	0.05	0.05

组别	例数	Parkin
对照组	3	17.92 ± 3.18
H₂O₂组	3	8.78 ± 4.09^#
Res组	3	16.56 ± 6.43^?
F值		23.72
P值		0.05

组别	例数	ROS	NLRP3	PINK1	Parkin	TNF-α	SOD2	IL-1β
对照组	3	12.71 ± 2.43	0.15 ± 0.05	0.97 ± 0.39	0.56 ± 0.24	0.61 ± 0.18	0.87 ± 0.26	0.30 ± 0.21
H₂O₂组	3	17.62 ± 4.78^#	0.35 ± 0.09^#	0.53 ± 0.35^#	0.18 ± 0.12^#	0.76 ± 0.32^#	0.58 ± 0.31^#	0.64 ± 0.28^#
PINK1（-）组	3	23.53 ± 5.36^?	0.66 ± 0.23^?	0.24 ± 0.18^?	0.09 ± 0.04^?	1.35 ± 0.45^?	0.32 ± 0.11^?	0.95 ± 0.45^?
Parkin（-）组	3	24.25 ± 4.93^?	0.85 ± 0.37^?	0.19 ± 0.08^?	0.04 ± 0.02^?	1.42 ± 0.39^?	0.13 ± 0.10^?	0.94 ± 0.37^?
F值		16.62	19.62	24.66	23.59	19.51	26.82	16.60
P值		0.05	0.05	0.05	0.05	0.05	0.05	0.05

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Effect of resveratrol mediated by the PINK1/Parkin pathway on fibroblast-like synovial cells of osteoarthritis treated with H2O2

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Effect of resveratrol mediated by the PINK1/Parkin pathway on fibroblast-like synovial cells of osteoarthritis treated with H₂O₂