苍术素调节CXCL12/CXCR4信号通路对哮喘幼年大鼠肺组织损伤的影响

doi:10.3969/j.issn.1006-5725.2024.19.002

Abstract

Abstract:

Objective To investigate the impact of Atractylodin on lung tissue damage in young asthmatic rats by regulating the CXC chemokine ligand 12 （CXCL12）/CXC chemokine receptor 4 （CXCR4） signaling pathway. Methods Twelve young SD rats were randomly selected from 60 rats as the control group （CON group）， while the remaining 48 rats were used to construct asthma models using ovalbumin （OVA）. Successfully modeled asthma rats were randomly separated into Model group， Atractylodin group （50 mg/kg Atractylodin）， and CXCL-12 group （5 μg/kg recombinant CXCL-12 protein） and Atractylodin+CXCL-12 group （50 mg/kg Atractylodin+5 μg/kg recombinant CXCL-12 protein）， with 12 in each group， continuously administered for 14 days. The CON and Model groups were given equal amounts of physiological saline. The percentages of neutrophils and eosinophils in bronchoalveolar lavage fluid （BALF） were detected. ELISA method was applied to detect cytokine levels in serum and BALF fluid； HE staining was applied to detect pathological changes in lung tissue； Western blot was applied to detect the levels of CXCL12/CXCR4 pathway related proteins. Results Compared with the CON group， the pathological score of lung tissue， percentage of neutrophils， percentage of eosinophils， the levels of IL-17， IL-4， IL-5， IL-13， IgE， OVA sIgE， and the protein levels of CXCL12 and CXCR4 in Model group were obviously increased （P < 0.05）； compared with the Model group， the pathological score of lung tissue， percentage of neutrophils， percentage of eosinophils， the levels of IL-17， IL-4， IL-5， IL-13， IgE， OVA sIgE， and the protein levels of CXCL12 and CXCR4 in the Atractylodin group were obviously reduced （P < 0.05）， the results in the CXCL-12 group were opposite to those in the Atractylodes group； CXCL-12 eliminated the improvement effect of atractylodes on asthma rats. Conclusion Atractylodin may improve lung tissue damage in asthmatic rats by down-regulating the CXCL12/CXCR4 signaling pathway.

Key words: Atractylodin, CXCL12/CXCR4 signaling pathway, asthma, lung damage

CLC Number:

R285.5

Yangyang CHEN,Hongqi MA,Jing YANG,Zongyue WU,Ping. ZHU. Impact of Atractylodin on lung tissue damage in young asthma rats by regulating the CXCL12/CXCR4 signaling pathway[J]. The Journal of Practical Medicine, 2024, 40(19): 2672-2677.

Figures/Tables 8

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References 20

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组别	嗜酸粒细胞	中性粒细胞
CON组	4.79 ± 0.58	10.47 ± 0.93
Model组	30.87 ± 0.46^*	24.48 ± 3.94^*
苍术素组	10.62 ± 1.15^△	12.36 ± 1.93^△
CXCL-12组	52.54 ± 4.74^△	33.36 ± 3.17^△
苍术素+CXCL-12组	27.67 ± 3.34^▲	28.31 ± 3.13^▲
F值	298.959	75.204
P值	< 0.001	< 0.001

组别	IL-13	IL-4	IL-5	IL-17
CON组	11.79 ± 2.58	16.53 ± 1.69	26.43 ± 1.76	36.90 ± 4.23
Model组	54.87 ± 6.46^*	48.58 ± 5.24^*	65.32 ± 5.88^*	68.56 ± 8.78^*
苍术素组	22.12 ± 3.35^△	24.35 ± 2.59^△	31.54 ± 2.76^△	43.09 ± 6.98^△
CXCL-12组	84.54 ± 9.34^△	65.85 ± 7.37^△	97.64 ± 8.12^△	96.54 ± 15.43^△
苍术素+CXCL-12组	43.67 ± 4.64^▲	43.36 ± 4.45^▲	52.46 ± 3.45^▲	56.23 ± 8.34^▲
F值	145.931	104.498	200.844	37.253
P值	< 0.001	< 0.001	< 0.001	< 0.001

组别	IL-13	IL-4	IL-5	IL-17
CON组	10.23 ± 2.70	15.69 ± 1.68	21.38 ± 3.27	41.79 ± 4.75
Model组	49.54 ± 6.50^*	43.93 ± 4.26^*	52.38 ± 6.37^*	64.87 ± 5.56^*
苍术素组	18.58 ± 3.35^△	22.47 ± 2.53^△	30.28 ± 3.87^△	42.12 ± 3.77^△
CXCL-12组	69.29 ± 9.37^△	61.49 ± 8.63^△	85.53 ± 9.39^△	84.12 ± 7.34^△
苍术素+CXCL-12组	41.58 ± 4.38^▲	39.38 ± 2.48^▲	48.56 ± 5.09^▲	63.67 ± 6.12^▲
F值	101.639	91.519	101.895	59.800
P值	< 0.001	< 0.001	< 0.001	< 0.001

组别	IgE	OVA sIgE
CON组	8.56 ± 0.83	0.00 ± 0.00
Model组	21.58 ± 1.37^*	19.63 ± 2.58^*
苍术素组	10.83 ± 0.83^△	5.48 ± 0.55^△
CXCL-12组	37.28 ± 4.63^△	35.83 ± 4.35^△
苍术素+CXCL-12组	18.72 ± 2.34^▲	12.36 ± 1.84^▲
F值	128.250	199.204
P值	< 0.001	< 0.001

组别	评分
CON组	0.06 ± 0.01
Model组	2.37 ± 0.29^*
苍术素组	0.74 ± 0.07^△
CXCL-12组	3.85 ± 0.42^△
苍术素+CXCL-12组	1.94 ± 0.25^▲
F值	198.884
P值	< 0.001

Impact of Atractylodin on lung tissue damage in young asthma rats by regulating the CXCL12/CXCR4 signaling pathway

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组别	CXCL12/GAPDH	CXCR4/GAPDH
CON组	0.33 ± 0.02	0.22 ± 0.02
Model组	0.65 ± 0.07^*	0.75 ± 0.09^*
苍术素组	0.42 ± 0.05^△	0.31 ± 0.02^△
CXCL-12组	0.93 ± 0.07^△	0.91 ± 0.08^△
苍术素+CXCL-12组	0.58 ± 0.06^▲	0.69 ± 0.07^▲
F值	99.147	130.812
P值	< 0.001	< 0.001

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