Abstract:

Objective To investigate the expression of GNA15 in peripheral blood in children with severe asthma（SA）and its prognostic value. Methods The dataset GSE27011 was downloaded from the Gene Expression Omnibus（GEO）of the National Center for Biotechnology Information in the United States. The key gene GNA15 was obtained by bioinformatics analysis. The medical records and blood samples of children were collected，and the expression levels of GNA15，inflammatory factors IL⁃4 and IL17 in peripheral blood，and respiratory function indexes were detected. The obtained data were used for univariate and multivariate analysis on prognosis assessment of children with SA.The receiver operating characteristic curve（ROC）was used to evaluate the efficacy of blood GNA15 in predicting SA children with poor prognosis. Results The expressions of GNA15 in peripheral mononu⁃ clear cells were gradually increased in the control group，MA group and SA group. The results of univariate analysis showed that GNA15 was an independent risk factor for poor prognosis in SA children（OR = 5.117）. GNA15 had a good efficacy in predicting poor prognosis of SA，with a sensitivity of 0.733，specificity of 0.879，and accuracy of 0.820. Conclusions GNA15 was highly expressed in peripheral blood，predicting a poor prognosis in children with SA.

Key words:

pediatric asthma, severe asthma, G protein subunit alpha 15, receiver operating char? acteristic curve, prognosis