TMSB10促进胃癌细胞增殖及糖酵解：基于激活AMPK/mTOR信号通路

doi:10.3969/j.issn.1006-5725.2024.11.009

Abstract

Abstract:

Objective To investigate the expression of Thymosinβ10 （TMSB10） in gastric cancer and its molecular mechanism in promoting the progression of gastric cancer. Methods We collected pathological sections of 70 patients with gastric cancer in our hospital， detected expression of TMSB10 in gastric adenocarcinoma by immunohistochemistry， and analyzed its prognostic effect. In order to study the mechanism of action， the effects of TMSB10 on the proliferation and glycolysis of gastric cancer cells and its related mechanism were observed by transfection technique， CCK8 test， EDU assay， Transwell assay， scratch test， glycolysis assay and Western blot. Results Immunohistochemistry showed that TMSB10was highly expressed in gastric cancer， while the expression level of TMSB10 was correlated with tumor size （P = 0.032）， TNM stage （P = 0.002） and distant metastasis. In the mechanism study， we found that overexpression of TMSB10 promoted the proliferation， invasion， migration and glycolytic phenotype of gastric cancer cells through in vitro CCK-8 test， EDU assay， Transwel assay and glycolysis assay， and vice versa. Western blot results showed that overexpression of TMSB10 may regulate the occurrence of gastric cancer through up-regulation of the AMPK/mTOR signaling pathway. Conclusions TMSB10 promotes the proliferation， invasion， migration and glycolysis gastric cancer through the AMPK/mTOR signaling pathway.

Key words: TMSB10, glycolysis, gastric cancer, AMPK/mTOR signaling pathway

CLC Number:

R735.2

Wei WANG,Xinxin ZHANG,Guanghui WANG,Jie ZHANG,Anran CHEN,Jianguang. JIA. TMSB10 promotes gastric cancer proliferation and glycolysis based on activation of AMPK/mTOR signaling pathway[J]. The Journal of Practical Medicine, 2024, 40(11): 1519-1525.

Figures/Tables 8

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References 23

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TMSB10表达	癌组织（n = 70）	癌旁组织（n = 70）	χ ² 值	P值
低表达	30（76.5）	65（27.1）	40.117	< 0.001
高表达	40（23.5）	5（72.9）

特征	例数	TMSB10 level		χ²值	P值
特征	例数	高	低	χ²值	P值
年龄				0.078	0.779
< 65岁	41	24	17
≥ 65岁	29	16	13
性别				0.172	0.678
男	37	22	15
女	33	18	15
T分期				0.045	0.832
T₁ - T₂	43	25	18
T₃ - T₄	27	15	12
分化程度				2.826	0.093
低分化	29	20	9
中高分化	41	20	21
肿瘤大小				4.580	0.032
< 5 cm	34	15	19
≥ 5 cm	36	25	11
TNM 分期				9.287	0.002
Ⅰ-Ⅱ期	32	12	20
Ⅲ-Ⅳ期	38	28	10
淋巴结转移				0.502	0.478
有	27	14	13
无	43	26	17
远处转移				13.266	< 0.001
无	41	16	25
育	29	24	5

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TMSB10 promotes gastric cancer proliferation and glycolysis based on activation of AMPK/mTOR signaling pathway

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