着丝粒蛋白F、miR-1-3p在中晚期胃癌患者血清中的表达及与预后的相关性

doi:10.3969/j.issn.1006-5725.2024.03.015

Abstract

Abstract:

Objective To investigate the expression of centromeric protein F （CENPF） and microribonucleic acid 1?3p （miR?1?3p） in the serum of patients with advanced gastric cancer and their correlation with prognosis. Methods Sixty patients with advanced gastric cancer admitted to our hospital from March 2019 to March 2020 were collected as the study group， while 60 healthy volunteers who underwent physical examinations at our hospital's physical examination center during the same period were collected as the control group. Real?time fluorescence quantitative PCR （qRT?PCR） method was applied to detect the expression levels of serum CENPF and miR?1?3p in each group； Pearson method was applied to analyze the correlation between serum levels of CENPF and miR?1?3p； Kaplan?Meier method was applied to analyze the relationship between the expression of CENPF， miR?1?3p， and prognosis in patients with advanced gastric cancer； and COX regression was applied to analyze risk factors affecting the prognosis of patients with advanced gastric cancer. Results Compared with the control group， the CENPF level in the study group was obviously increased， while the miR?1?3p level was obviously reduced （P < 0.05）. The correlation analysis results showed that there was a negative correlation between serum CENPF and miR?1?3p levels in patients with advanced gastric cancer （r = -0.650，P < 0.001）. There were obvious differences in CENPF and miR?1?3p levels among different TNM stages and lymph node metastasis status （P < 0.05）. The 3?year survival rate of patients in the high expression group of CENPF was 19/30 （63.33%）， which was obviously lower than that in the low expression group， 28/30 （93.33%）（χ² = 7.954， P < 0.001）； the 3?year survival rate of patients in high expression group of miR?1?3p was 29/30 （96.67%）， which was obviously higher than that in the low expression group， 18/30 （60.00%）（χ² = 11.882， P = 0.001）. Multivariate COX regression analysis showed that TNM staging， lymph node metastasis， CENPF， and miR?1?3p expression were risk factors affecting the prognosis of patients with advanced gastric cancer（P < 0.05）. Conclusion The serum CENPF level in patients with advanced gastric cancer obviously increase， while miR?1?3p level obviously decrease， both of which are related to prognosis.

Key words: Centromeric protein F, Micro ribonucleic acid?1?3p, Middle to late stage gastric cancer, Prognosis

CLC Number:

R735.2

Jian ZHAO,Songjie LIU,Guanchao ZHANG,Yuhou SHEN,Fengchen LI,Bing XU. Expression of CENPF and miR⁃1⁃3p in the serum of patients with advanced gastric cancer and their correlation with prognosis[J]. The Journal of Practical Medicine, 2024, 40(3): 365-370.

Figures/Tables 9

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References 25

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组别	例数	性别（男/女，例）	年龄（岁）	BMI（kg/m²）	TNM分期（例）			淋巴结转移（例）
组别	例数	性别（男/女，例）	年龄（岁）	BMI（kg/m²）	Ⅱb	Ⅲ期	Ⅳ期	淋巴结转移（例）
对照组	60	33/27	62.20 ± 6.54	22.17 ± 2.42	-	-	-	-
研究组	60	32/28	62.30 ± 6.48	22.15 ± 2.36	19	24	17	18
χ ²/t值		0.034	0.084	0.046
P值		0.855	0.933	0.964

基因	正向引物5′-3′	反向引物5′-3′
CENPF	AGCACGACTCCAGCTACAAGGT	CATCA TGCTTTGGTGTTCTTTG
miR-1-3p	ACACTCCAGGTGGGTGGAATGT	CTCAACTGGTGTGTCGTGGAG
GAPDH	TGACTTCAACAGCGA CACCCA	CACCCTGTTGCTGTAGCCAAA
U6	CTCGCTTCGGCAGCACATA	AACGATTCACGAATTTGCGT

组别	例数	CENPF/GAPDH	miR-1-3p/U6
对照组	60	1.02 ± 0.11	1.01 ± 0.12
研究组	60	1.35 ± 0.21	0.75 ± 0.14
t值		10.783	10.922
P值		< 0.001	< 0.001

病理特征	例数	CENPF	t值	P值	miR-1-3p	t值	P值
年龄
> 60岁	33	1.34 ± 0.22	0.365	0.717	0.76 ± 0.13	0.553	0.582
≤ 60岁	27	1.36 ± 0.20	0.365	0.717	0.74 ± 0.15	0.553	0.582
性别
男	32	1.35 ± 0.22	0.183	0.855	0.75 ± 0.15	0.274	0.785
女	28	1.36 ± 0.20	0.183	0.855	0.74 ± 0.13	0.274	0.785
BMI
> 22 kg/m²	33	1.36 ± 0.21	0.359	0.721	0.76 ± 0.13	0.553	0.582
≤ 22 kg/m²	27	1.34 ± 0.22	0.359	0.721	0.74 ± 0.15	0.553	0.582
肿瘤最大径
> 4 cm	35	1.37 ± 0.21	0.713	0.479	0.74 ± 0.14	0.562	0.576
< 4 cm	25	1.33 ± 0.22	0.713	0.479	0.76 ± 0.13	0.562	0.576
TNM分期
Ⅱb期	19	1.20 ± 0.20	10.45	< 0.001	0.87 ± 0.15	14.243	< 0.001
Ⅲ期	24	1.36 ± 0.21^*			0.74 ± 0.14^*
Ⅳ期	17	1.52 ± 0.22^*#			0.62 ± 0.13^*#
淋巴结转移
是	18	1.48 ± 0.20	3.085	0.003	0.60 ± 0.13	5.694	< 0.001
否	42	1.30 ± 0.21	3.085	0.003	0.82 ± 0.14	5.694	< 0.001

影响因素	单因素分析				多因素分析
影响因素	SE	HR	95% CI	P值	SE	HR	95% CI	P值
TNM分期	0.252	2.652	1.618 ~ 4.346	< 0.001	0.351	2.054	1.032 ~ 4.087	0.040
淋巴结转移	0.187	3.986	2.763 ~ 5.751	< 0.001	0.154	1.943	1.437 ~ 2.628	< 0.001
CENPF	0.174	4.028	2.864 ~ 5.665	< 0.001	0.278	3.641	2.111 ~ 6.279	< 0.001
miR-1-3p	0.264	2.954	1.761 ~ 4.956	< 0.001	0.325	5.263	2.783 ~ 9.951	< 0.001

Expression of CENPF and miR⁃1⁃3p in the serum of patients with advanced gastric cancer and their correlation with prognosis

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