趋化因子CXCL5与慢性萎缩性胃炎及胃癌前病变的相关性

doi:10.3969/j.issn.1006-5725.2024.15.009

Abstract

Abstract:

Objective To clarify the changes in CXCL5 in serum and gastric tissues of patients with chronic atrophic gastritis （CAG） and precancerous lesions of gastric cancer （PLGC） and to investigate the predictive value of CXCL5 for the diagnosis of CAG and PLGC. Methods This study enrolled 72 participants of CAG admitted to the Department of Splenology and Gastroenterology of the Second Affiliated Hospital of Anhui University of Chinese Medicine from June 2022 to June 2023， with gastroscopy and pathologically confirmed diagnosis， as well as 68 healthy participants who underwent gastroscopy in the same period at our department.We collected clinical information and laboratory results from all participants. The logistic regression analysis methods were used to identify the diagnostic value of serum CXCL5. Furthermore， in order to clarify the role of CXCL5 in the developent of CAG， a total of 15 patients each with CAG， intestinal metaplasia， and dysplasia treated at our hospital from June 2023 to December 2023， and 15 healthy participants were selected. The relationship between the expression of CXCL5 and the degree of clinicopathology was analysed in each group using ELISA， PCR， and immunohistochemistry staining to validate and assess the diagnostic efficacy of CXCL5. Results The study found that several factors were associated with CAG， including family history of tumours， smoking and alcohol consumption history， dietary regularity， Helicobacter pylori infection， the number of lesions， gastric function scores and CXCL5 （P < 0.05）. The ROC curve had an AUC of 1.00 and a Youden index of 0.986， indicating excellent predictive ability. The ELISA results indicated a significantly higher serum CXCL5 expression level in the CAG， intestinal metaplasia， and dysplasia groups compared to the normal group. There was a positive correlation between the serum CXCL5 expression level and the degree of pathology. The PCR and immunohistochemistry staining results indicate that the mRNA and protein expression levels of CXCL5 in gastric tissues of patient groups were significantly higher compared to the normal group. Furthermore， the mRNA and protein expression levels of CXCL5 in gastric tissues were positively correlated with the degree of pathology. Conclusions The results indicate that CXCL5 is highly expressed in the serum and gastric tissues of patients with CAG and PLGC， and its expression level is positively correlated with the degree of pathology. Therefore， CXCL5 could serve as a predictive indicator and a potential therapeutic target for the diagnosis of CAG and PLGC.

Key words: chronic atrophic gastritis, CXCL5, precancerous lesions of gastric cancer, diagnostic value

CLC Number:

R573.3⁺1

Bei PEI,Yi ZHANG,Qin SUN,Yueping JIN,Xuejun. LI. Study of the association between the chemokine CXCL5 and the onset of chronic atrophic gastritis and gastric precancerous lesions[J]. The Journal of Practical Medicine, 2024, 40(15): 2098-2104.

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References 33

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基因	扩增片段大小（bp）	上游引物（5'→3'）	下游引物（5'→3'）
β-actin	96	CCCTGGAGAAGAGCTACGAG	GGAAGGAAGGCTGGAAGAGT
CXCL5	74	TTTTGGACGGTGGAAACAAG	TGCTGAAGACTGGGAAACTT

指标	正常组	萎缩组	t/χ²值	P值
年龄（x ± s，岁）	53.99 ± 10.04	56.89 ± 8.04	-1.895	0.060
性别			0.50	0.478
男	29	35
女	39	37
BMI（x ± s，kg/m²）	22.66 ± 2.24	22.96 ± 3.09	-0.649	0.518
吸烟史			4.37	0.037
有	7	17
无	61	55
饮酒史			5.27	0.022
有	9	21
无	59	51
饮食习惯			10.17	0.001
不规律	0	10
规律	68	62
糖尿病			0.61	0.434
有	9	13
无	59	59
高血压			0.68	0.410
有	21	27
无	47	45
高脂血症			0.07	0.796
有	25	28
无	43	44
肿瘤家族史			5.40	0.020
有	1	8
无	67	64
幽门螺杆菌感染			52.30	< 0.000 1
阳性	5	48
阴性	63	24
病灶数量			49.00	< 0.000 1
一处	0	40
两处	0	10
三处	0	22
病理程度			56.00	< 0.000 1
萎缩	0	26
伴肠化	0	31
伴异型增生	0	15

指标	正常组	萎缩组	t值	P值
G17（pmol/L）	8.57 ± 5.81	3.91 ± 2.99	6.004	< 0.000 1
PG1（μg/L）	119.48 ± 38.44	81.39 ± 46.04	5.298	< 0.000 1
PG2（μg/L）	11.22 ± 6.77	8.29 ± 4.91	2.946	< 0.000 1
CXCL5（pg/mL）	1 783.95 ± 71.35	4 437.61 ± 825.67	-26.405	< 0.000 1

指标	曲线下面积(AUC)	标准误差	约登指数	95%CI
吸烟史	0.567	0.048	0.133	0.472 ~ 0.622
饮酒史	0.580	0.048	0.160	0.485 ~ 0.674
饮食习惯	0.569	0.048	0.139	0.475 ~ 0.664
肿瘤家族史	0.562	0.048	0.124	0.467 ~ 0.657
幽门螺杆菌感染	0.797	0.039	0.593	0.720 ~ 0.873
病灶数目	1.000	< 0.001	1.000	1.000 ~ 1.000
病理程度	1.000	< 0.001	1.000	1.000 ~ 1.000
G17	0.218	0.038	0.001	0.142 ~ 0.293
PG1	0.199	0.040	0.042	0.121 ~ 0.276
PG2	0.355	0.046	0.001	0.265 ~ 0.446
CXCL5	1.000	< 0.001	0.986	1.000 ~ 1.000

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Study of the association between the chemokine CXCL5 and the onset of chronic atrophic gastritis and gastric precancerous lesions

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