男性不育患者血清NLRP3和PTEN表达与性激素水平及精液质量的关系

doi:10.3969/j.issn.1006-5725.2023.21.005

Abstract

Abstract:

Objective To investigate the relationship of the expression of nucleotide?binding oligomerization domain?like receptor protein 3 （NLRP3） and phosphatase and tensin homolog deleted on chromosome 10 （PTEN） in the serum of male infertility patients with the levels of sex hormones and semen quality. Methods A total of 80 male infertile patients admitted to our hospital from May 2021 to April 2023 were enrolled as the research subjects， who were divided into oligozoospermia group （n = 47） and asthenozoospermia group （n = 33） based on semen quality. Meanwhile， 50 male healthy patients who underwent health examinations in our hospital during the same period were recruited as the control group. The level of serum NLRP3 and PTEN were determined by ELISA， and their relations with the sex hormone levels and semen quality were analyzed. Results Compared with the control group， the semen quality as well as the levels of testosterone （T） and serum PTEN in the patients were significantly reduced （P <0.05）， while the levels of follicle?stimulating hormone （FSH）， luteinizing hormone （LH）， estradiol （E2）， prolactin （PRL） and serum NLRP3 were significantly increased （P < 0.05）. Compared with the oligospermia group， the sperm concentration， semen volume， and the levels of FSH， T， serum NLRP3， and PTEN in the asthenospermia group were significantly increased （P < 0.05）， the sperm motility and forward motility sperm rate （PR） were significantly decreased （P < 0.05）， and there were no significant differences in the levels of LH， E2， and PRL （P > 0.05）. Through Pearson correlation analysis， the level of serum NLRP3 was negatively correlated with the semen volume， sperm motility， and the levels of PR and T， and significantly positively correlated with the levels of FSH， LH， E2， and PRL （P < 0.05）； The PTEN expression was significantly positively correlated with the sperm concentration， semen volume， sperm motility and the levels of PR and T， and negatively correlated with the levels of FSH， LH， E2， and PRL （P < 0.05）. Conclusion The level of serum NLRP3 is decreased and the PTEN level is increased in male infertile patients. Both of them are correlated with sex hormones and semen quality.

Key words: male infertility, semen quality, NLRP3, PTEN

CLC Number:

R698.2.

Yaoning SHAO,Fuxian XIE,Haiyang HUANG. The relationship of serum NLRP3 and PTEN expression with sex hormone level and semen quality in male infertile patients[J]. The Journal of Practical Medicine, 2023, 39(21): 2718-2722.

Figures/Tables 4

Tab.1

Tab.2

Tab.3

Tab.4

References 25

1	LLAVANERA M ， DELGADO-BERMÚDEZ A ， RIBAS-MAYNOU J ， et al . A systematic review identifying fertility biomarkers in semen： a clinical approach through Omics to diagnose male infertility［J］. Fertil Steril， 2022， 118（2）：291-313.
2	MURSHIDI M M ， CHOY J T ， EISENBERG M L . Male infertility and somatic health［J］. Urol Clin North Am， 2020， 47（2）：211-217.
3	NUNES D C ， RIBEIRO J C ， ALVES M G ， et al . Male sex hormones， metabolic syndrome， and aquaporins： a triad of players in male （in） fertility［J］. Int J Mol Sci， 2023， 24（3）：1960.
4	NUNES D C ， RIBEIRO J C ， ALVES M G ， et al . Male sex hormones， metabolic syndrome， and aquaporins： a triad of players in male （in） fertility［J］. Int J Mol Sci， 2023， 24（3）：1960.
5	ZAŃKO A ， SIEWKO K ， KRĘTOWSKI A J ， et al . Lifestyle， insulin resistance and semen quality as co-dependent factors of male infertility［J］. Int J Environ Res Public Health， 2022， 20（1）：732.
6	FIJAK M ， PILATZ A ， HEDGER M P ， et al . Infectious， inflammatory and 'autoimmune' male factor infertility： how do rodent models inform clinical practice？［J］. Hum Reprod Update， 2018， 24（4）：416-441.
7	BARATI E ， NIKZAD H ， KARIMIAN M . Oxidative stress and male infertility： current knowledge of pathophysiology and role of antioxidant therapy in disease management［J］. Cell Mol Life Sci， 2020， 77（1）：93-113.
8	SANO M ， KOMIYAMA H ， SHINODA R ， et al . NLRP3 inflammasome is involved in testicular inflammation induced by lipopolysaccharide in mice［J］. Am J Reprod Immunol， 2022， 87（4）：e13527.
9	范宇 . 睾酮缺乏诱导miR-26a-5p和let-7G-5p作为信号逆质靶向作用于PTEN和PMAIP1调控公猪精子细胞凋亡［D］. 成都：四川农业大学，2018.
10	BJÖRNDAHL L ， KIRKMAN BROWN J ，other editorial board members of the WHO laboratory manual for the examination and processing of human semen . The sixth edition of the WHO laboratory manual for the examination and processing of human semen： ensuring quality and standardization in basic examination of human ejaculates［J］. Fertil Steril， 2022， 117（2）：246-251.
11	KURKOWSKA W ， BOGACZ A ， JANISZEWSKA M ， et al . Oxidative stress is associated with reduced sperm motility in normal semen［J］. Am J Mens Health， 2020， 14（5）：1557988320939731.
12	VASHISHT A ， GAHLAY G K . Using miRNAs as diagnostic biomarkers for male infertility： opportunities and challenges［J］. Mol Hum Reprod， 2020， 26（4）：199-214.
13	ASADPOUR R ， MOFIDI CHELAN E . Using microRNAs as molecular biomarkers for the evaluation of male infertility［J］. Andrologia， 2022， 54（2）：e14298.
14	AGARWAL A ， RANA M ， QIU E ， et al . Role of oxidative stress， infection and inflammation in male infertility［J］. Andrologia， 2018， 50（11）：e13126.
15	IHSAN A U ， KHAN F U ， KHONGORZUL P ， et al . Role of oxidative stress in pathology of chronic prostatitis/chronic pelvic pain syndrome and male infertility and antioxidants function in ameliorating oxidative stress［J］. Biomed Pharmacother， 2018， 106：714-723.
16	WANG Y ， LIU X ， SHI H ， et al . NLRP3 inflammasome， an immune-inflammatory target in pathogenesis and treatment of cardiovascular diseases［J］. Clin Transl Med， 2020， 10（1）：91-106.
17	KELLEY N ， JELTEMA D ， DUAN Y ， et al . The NLRP3 Inflammasome： An Overview of Mechanisms of Activation and Regulation［J］. Int J Mol Sci， 2019， 20（13）：3328.
18	HUGHES M M ， O'NEILL L A J . Metabolic regulation of NLRP3［J］. Immunol Rev， 2018， 281（1）：88-98.
19	郑建波，陈辉，郑其梅，等 . 男性不育症患者抗米勒管激素水平与性激素、精液参数水平的相关性分析［J］. 中国性科学， 2021， 30（1）：7-9.
20	LU J ， SU Y ， CHEN X ， et al Rapamycin-induced autophagy attenuates hormone-imbalance-induced chronic non-bacterial prostatitis in rats via the inhibition of NLRP 3 inflammasome-mediated inflammation［J］. Mol Med Rep， 2019， 19（1）：221-230.
21	KHODAMORADI K ， AMINI-KHOEI H ， KHOSRAVIZADEH Z ， et al . Oxidative stress， inflammatory reactions and apoptosis mediated the negative effect of chronic stress induced by maternal separation on the reproductive system in male mice［J］. Reprod Biol， 2019， 19（4）：340-348.
22	ASADI A ， GHAHREMANI R ， ABDOLMALEKI A ， et al . Role of sperm apoptosis and oxidative stress in male infertility： A narrative review［J］. Int J Reprod Biomed， 2021， 19（6）：493-504.
23	QIAN Y C ， XIE Y X ， WANG C S ， et al . Mkrn2 deficiency induces teratozoospermia and male infertility through p53/PERP-mediated apoptosis in testis［J］. Asian J Androl， 2020， 22（4）：414-421.
24	WISE H M ， HARRIS A ， KRIPLANI N ， et al . PTEN protein phosphatase activity is not required for tumour suppression in the mouse prostate［J］. Biomolecules， 2022， 12（10）：1511.
25	陶明进，曹艳 . 血清性激素指标、精浆锌与果糖检测诊断男性不育症的应用价值［J］. 中国妇幼保健， 2019， 34（9）：2090-2092.

组别	例数	精子浓度（×10⁶个/mL）	精液体积（mL）	精子活力（%）	PR（%）
少精子组	47	1.09 ± 0.27^ab	2.84 ± 0.65^ab	48.17 ± 7.02^ab	49.47 ± 7.69^ab
弱精子组	33	5.91 ± 0.94^a	3.43 ± 0.41^a	26.16 ± 3.44^a	28.96 ± 3.27^a
对照组	50	6.41 ± 1.17	4.29 ± 0.52	53.46 ± 7.28	53.71 ± 7.33
F值		509.503	85.993	190.713	146.270
P值		< 0.001	< 0.001	< 0.001	< 0.001

组别	例数	FSH（IU/L）	T（nmol/L）	LH（IU/L）	E₂（pmol/L）	PRL（μIU/mL）
少精子组	47	8.49 ± 2.21^ab	4.82 ± 0.77^ab	4.96 ± 1.68^a	146.58 ± 12.17^a	199.27 ± 65.16^a
弱精子组	33	11.51 ± 2.84^a	5.76 ± 0.93^a	5.31 ± 1.83^a	150.62 ± 13.01^a	251.21 ± 66.73^a
对照组	50	5.83 ± 1.57	7.24 ± 1.19	4.17 ± 1.26	140.37 ± 12.13	165.83 ± 53.14
F值		67.988	73.648	5.898	7.269	19.326
P值		< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

组别	例数	NLRP3（pg/mL）	PTEN（ng/mL）
少精子组	47	108.32 ± 14.81^ab	1.98 ± 0.49^ab
弱精子组	33	130.54 ± 21.33^a	2.55 ± 0.63^a
对照组	50	90.13 ± 11.28	4.07 ± 1.50
F值		67.026	53.153
P值		< 0.001	< 0.001

指标	NLRP3		PTEN
指标	r值	P值	r值	P值
精子浓度	-0.156	0.075	0.639	< 0.001
精液体积	-0.431	< 0.001	0.665	< 0.001
精子活力	-0.686	< 0.001	0.435	< 0.001
PR	-0.691	< 0.001	0.411	0.001
FSH	0.690	< 0.001	-0.574	< 0.001
T	-0.467	< 0.001	0.671	< 0.001
LH	0.699	< 0.001	-0.406	< 0.001
E₂	0.581	< 0.001	-0.402	< 0.001
PRL	0.504	< 0.001	-0.402	< 0.001

[1]	Fu CHEN,Bin LIU,Shuaijun HE,Yong ZHAO,Weizhou. WANG. The relationship between semen quality and trace element levels in seminal plasma and miR⁃184 levels in seminal vesicles of male infertility patients [J]. The Journal of Practical Medicine, 2024, 40(7): 930-935.
[2]	Kaiqiang WANG,Zhi CAO,Wei WANG,Chenglin YANG,Yue YANG,Huifen ZHANG,Xaioming ZHANG. Effects of Diosmine on semen quality and sex hormone concentration in patients with spermatovenous ligation [J]. The Journal of Practical Medicine, 2024, 40(3): 389-393.
[3]	Xiuli SHI,Jiaqi CHEN,Fan ZHU,Juan ZENG,Na. WU. The effect of miR⁃143⁃3p on pyroptosis of ulcerative colitis cells by regulating TLR2/NF⁃κB/NLRP3 [J]. The Journal of Practical Medicine, 2024, 40(15): 2056-2062.
[4]	Yubo GONG,Xiaohua GUO,Wenjun LU,Yuanchao LI,Changyu QIU,Yuanyuan SHI,Liping XIA,Lin SHI,Wei WU,Ling. LUO. The role of NLRP3 signaling pathway in allergic rhinoconjunctivitis [J]. The Journal of Practical Medicine, 2024, 40(14): 1922-1927.
[5]	Mengni HU,Xiaolei ZHANG,Zhen RONG,Yao WANG,Ya′nan LI,Jun. MA. Effects of electroacupuncture on MPTP⁃induced FoXO1/NLRP3 pathway mediated neuroinflammation in mice with Parkinson′s disease [J]. The Journal of Practical Medicine, 2024, 40(11): 1494-1499.
[6]	Linhui PENG,Dan LI,Zhiqiang HU,Xialin. ZUO. Connexin 43 hemichannel mediates NLRP3 inflammasome activation and its role in cerebral ischemia [J]. The Journal of Practical Medicine, 2024, 40(10): 1450-1454.
[7]	TANG Yuting, MA Fang, HE Xi, WANG Jianjun, YANG Anning, WU Kai, JIAO Yun, BAI Zhigang, JIANG Yideng, SHEN Jiangyong. . Shikonin inhibits collagen deposition of human hypertrophic scar fibroblasts via PTEN [J]. The Journal of Practical Medicine, 2023, 39(11): 1382-1388.
[8]	WU Yanli, ZHOU Wenjie, LI Xiaoling, MA Xigang.. Correlation between serum NOD⁃like receptor protein 3，interleukin⁃18 and severity of septicacute kidneyinjury [J]. The Journal of Practical Medicine, 2022, 38(4): 484-489.
[9]	LU Dongping, ZHANG Rong, WANG Fen, WEI Shaofeng, JIA Jie, ZHANG Weilian, LIU Ting, LIANG Rui, YANG Wenzhi. Expression of NLRP3 inflammasome and cytokines in patients with syphilis Serofast state [J]. The Journal of Practical Medicine, 2021, 37(8): 1041-1045.
[10]	LIU Xin, CAO Wenwen, BAI Bing. . Association of TNF⁃α，NLRP3，CRP/ALB with the severity of carbapenem⁃resistant Klebsiellapneumoni⁃ ae infection in the elderly and their influence on prognosis #br# #br# [J]. The Journal of Practical Medicine, 2021, 37(24): 3174-3178.
[11]	FAN Xiaomeng, ZHU Hongwei, ZHU Zhimin, QIU Aiping, LIU Yongsheng. . Clinical application value of highly selective spermatic vein ligation under the outer ring microscope [J]. The Journal of Practical Medicine, 2021, 37(16): 2124-2128.
[12]	LUO Ying, XU Jianying, LIN Xiaomin, JIN Long, QU Shihao.. Correlation between sperm DNA fragmentation index and male infertility or early recurrent abortion occurred in the subjects′ spouses [J]. The Journal of Practical Medicine, 2021, 37(16): 2133-2136.
[13]	MO Zhiyang, LI Ying, HAN Xiaodong, WANG Hongxia, WENG Qiaofeng. Icariin Ⅱ inhibits epithelial⁃mesenchymal transition in tongue squamous cell carcinoma [J]. The Journal of Practical Medicine, 2020, 36(22): 3069-3073.
[14]	LU Jiawei, LIU Xiaogu, ZHANG Wenbo. The regulatory mechanism of lncRNA and miRNA on NLRP3 inflammasome signal and non⁃coding RNA′s role in associated diseases [J]. The Journal of Practical Medicine, 2020, 36(22): 3149-3152.

The relationship of serum NLRP3 and PTEN expression with sex hormone level and semen quality in male infertile patients

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 25

Related Articles 14

Recommended Articles

Metrics

Comments