男性不育患者精液质量与精浆微量元素水平及精浆外泌体miR-184水平的关系

doi:10.3969/j.issn.1006-5725.2024.07.009

Abstract

Abstract:

Objective Explore the relationship between semen quality and trace element levels in seminal plasma， as well as miR-184 levels in exosomes of male infertility patients. Methods 155 male infertility patients in our hospital from January 2022 to July 2023 were selected as the infertility group， and another 155 male patients with normal physical examination during the same period were selected as the normal group. Compare the general information， semen quality parameters， trace elements， and miR-184 levels in exosomes between two groups， and analyze the correlation between male infertility patients and the above laboratory indicators through Spearman rank correlation analysis. Results The sperm concentration， total sperm motility （PR + NP）， forward motility sperm （PR）， average curve velocity， average linear velocity， average path velocity， and average lateral amplitude of sperm head （ALH） in the infertile group were lower than those in the normal group （P < 0.05）； The levels of Zn， Mg， and Ca in the infertile group were lower than those in the normal group， while the levels of Cu， Pb， and relative expression of miR-184 were higher than those in the normal group （P < 0.05）； The levels of Zn and Cu elements in male infertility patients are positively and negatively correlated with sperm concentration， total sperm motility （PR+NP）， and forward motility sperm （PR）， respectively. The levels of Pb elements are negatively correlated with the average lateral amplitude of sperm head （ALH）， and P < 0.05. The relative expression level of miR-184 in male infertility patients is negatively correlated with sperm concentration， total sperm motility （PR + NP）， forward motility sperm （PR）， and average sperm curve velocity， with P < 0.05. Conclusion The quality of semen in male infertility patients is closely related to the levels of trace elements in semen and miR-184 levels in exosomes.

Key words: male infertility, semen quality, trace elements in seminal plasma, seminal plasma, exosomes

CLC Number:

R698⁺.2

Fu CHEN,Bin LIU,Shuaijun HE,Yong ZHAO,Weizhou. WANG. The relationship between semen quality and trace element levels in seminal plasma and miR⁃184 levels in seminal vesicles of male infertility patients[J]. The Journal of Practical Medicine, 2024, 40(7): 930-935.

Figures/Tables 5

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References 31

1	KALE S， RASHID T. Feasibility of loupe assisted subinguinal varicocelectomy in treatment of male infertility［J］. Actas Urol Esp （Engl Ed）， 2022，46（9）：515-520. doi:10.1016/j.acuroe.2022.02.005 doi: 10.1016/j.acuroe.2022.02.005
2	CILLIERS K. Trace element alterations in Alzheimer's disease： A review［J］. Clin Anat， 2021，34（5）：766-773. doi:10.1002/ca.23727 doi: 10.1002/ca.23727
3	KARABULUT S， KORKMAZ S， GÜNEŞ E， et al. Seminal trace elements and their relationship with sperm parameters［J］. Andrologia， 2022，54（11）：14610. doi:10.1111/and.14610 doi: 10.1111/and.14610
4	BI Y， QIAO X， LIU Q， et al. Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells［J］. Stem Cell Res Ther， 2022，13（1）：449. doi:10.1186/s13287-022-03142-1 doi: 10.1186/s13287-022-03142-1
5	朱媛媛，卞玉莹，顾万建，等. 男性不育患者精浆exosome中miR-184水平变化及临床意义［J］. 中华男科学杂志， 2020，26（8）：686-694.
6	《男性生殖遗传学检查专家共识》编写组，中华医学会男科学分会.男性生殖遗传学检查专家共识［J］. 中华男科学杂志，2015，21（12）：1138-1142.
7	陈振文，谷龙杰. 精液分析标准化和精液质量评估——WHO《人类精液检查与处理实验室手册》（第5版）出版［J］. 中国计划生育学杂志， 2012，20（1）：58-62. doi:10.3969/j.issn.1004-8189.2012.01.019 doi: 10.3969/j.issn.1004-8189.2012.01.019
8	MANOUCHEHRI A， SHOKRI S， PIRHADI M， et al. The Effects of Toxic Heavy Metals Lead， Cadmium and Copper on the Epidemiology of Male and Female Infertility［J］. JBRA Assist Reprod， 2022，26（4）：627-630.
9	MELNIK B C， STREMMEL W， WEISKIRCHEN R， et al. Exosome-Derived MicroRNAs of Human Milk and Their Effects on Infant Health and Development［J］. Biomolecules， 2021，11（6）：851. doi:10.3390/biom11060851 doi: 10.3390/biom11060851
10	VICKRAM A S， SRIKUMAR P S， SRINIVASAN S， et al. Seminal exosomes-An important biological marker for various disorders and syndrome in human reproduction［J］. Saudi J Biol Sci，2021，28（6）：3607-3615. doi:10.1016/j.sjbs.2021.03.038 doi: 10.1016/j.sjbs.2021.03.038
11	AGARWAL A， BASKARAN S， PAREKH N， et al. Male infertility［J］. Lancet， 2021，397（10271）：319-333. doi:10.1016/s0140-6736(20)32667-2 doi: 10.1016/s0140-6736(20)32667-2
12	MINHAS S， BETTOCCHI C， BOERI L， et al. European Association of Urology Guidelines on Male Sexual and Reproductive Health： 2021 Update on Male Infertility［J］. Eur Urol， 2021，80（5）：603-620. doi:10.1016/j.eururo.2021.08.014 doi: 10.1016/j.eururo.2021.08.014
13	ZAMBRANO SERRANO C A， CARVAJAL OBANDO A. Diagnosis and hormonal treatment of male infertility［J］. Actas Urol Esp （Engl Ed）， 2020，44（5）：321-327. doi:10.1016/j.acuroe.2020.05.007 doi: 10.1016/j.acuroe.2020.05.007
14	朱媛媛，卞玉莹，顾万建，等. 男性不育患者精浆exosome中miR-184水平变化及临床意义［J］. 中华男科学杂志， 2020，26（8）：686-694.
15	WANG Y Y， DUAN S H， WANG G L， et al. Integrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii［J］. Sci Rep， 2021，11（1）：665. doi:10.1038/s41598-020-80264-7 doi: 10.1038/s41598-020-80264-7
16	BURGOS C F， CIKUTOVIC R， ALARCÓN M. MicroRNA expression in male infertility［J］. Reprod Fertil Dev， 2022，34（12）：805-818. doi:10.1071/rd21131 doi: 10.1071/rd21131
17	BARBU M G， THOMPSON D C， SUCIU N， et al. The Roles of MicroRNAs in Male Infertility［J］. Int J Mol Sci， 2021，22（6）：2910. doi:10.3390/ijms22062910 doi: 10.3390/ijms22062910
18	BEIGI HARCHEGANI A， DAHAN H， TAHMASBPOUR E， et al. Effects of zinc deficiency on impaired spermatogenesis and male infertility： the role of oxidative stress， inflammation and apoptosis［J］. Hum Fertil （Camb）， 2020，23（1）：5-16. doi:10.1080/14647273.2018.1494390 doi: 10.1080/14647273.2018.1494390
19	SCHISTERMAN E F， SJAARDA L A， CLEMONS T， et al. Effect of Folic Acid and Zinc Supplementation in Men on Semen Quality and Live Birth Among Couples Undergoing Infertility Treatment： A Randomized Clinical Trial［J］. JAMA， 2020，323（1）：35-48. doi:10.1001/jama.2019.18714 doi: 10.1001/jama.2019.18714
20	VICKRAM S， ROHINI K， SRINIVASAN S， et al. Role of Zinc （Zn） in Human Reproduction： A Journey from Initial Spermatogenesis to Childbirth［J］. Int J Mol Sci， 2021，22（4）：2188. doi:10.3390/ijms22042188 doi: 10.3390/ijms22042188
21	CHABCHOUB I， NOUIOUI M A， ARAOUD M， et al. Effects of lead， cadmium， copper and zinc levels on the male reproductive function［J］. Andrologia， 2021，53（9）：e14181. doi:10.1111/and.14181 doi: 10.1111/and.14181
22	NUNZIO A D， GIARRA A， TOSCANESI M， et al. Comparison between Macro and Trace Element Concentrations in Human Semen and Blood Serum in Highly Polluted Areas in Italy［J］. Int J Environ Res Public Health， 2022，19（18）：11635. doi:10.3390/ijerph191811635 doi: 10.3390/ijerph191811635
23	MOJADADI A， AU A， SALAH W， et al. Role for Selenium in Metabolic Homeostasis and Human Reproduction［J］. Nutrients，2021，13（9）：3256. doi:10.3390/nu13093256 doi: 10.3390/nu13093256
24	OMMATI M M， AHMADI H N， SABOURI S， et al. Glycine protects the male reproductive system against lead toxicity via alleviating oxidative stress， preventing sperm mitochondrial impairment， improving kinematics of sperm， and blunting the downregulation of enzymes involved in the steroidogenesis［J］. Environ Toxicol， 2022，37（12）：2990-3006. doi:10.1002/tox.23654 doi: 10.1002/tox.23654
25	罗英，徐楗荧，林小民，等. 精子DNA碎片率与男性不育及其配偶早期复发性流产的关系［J］. 实用医学杂志， 2021，37（16）：2133-2136. doi:10.3969/j.issn.1006-5725.2021.16.020 doi: 10.3969/j.issn.1006-5725.2021.16.020
26	FOROUHARI S， MAHMOUDI E， SAFDARIAN E， et al. MicroRNA： A Potential Diagnosis for Male Infertility［J］. Mini Rev Med Chem， 2021，21（10）：1226-1236. doi:10.2174/1389557520999201209213319 doi: 10.2174/1389557520999201209213319
27	GRIECO G E， BRUSCO N， FIGNANI D， et al. Reduced miR-184-3p expression protects pancreatic β-cells from lipotoxic and proinflammatory apoptosis in type 2 diabetes via CRTC1 upregulation［J］. Cell Death Discov， 2022，8（1）：340. doi:10.1038/s41420-022-01142-x doi: 10.1038/s41420-022-01142-x
28	JOSHI M， ANDRABI S W， YADAV R K， et al. Qualitative and quantitative assessment of sperm miRNAs identifies hsa-miR-9-3p， hsa-miR-30b-5p and hsa-miR-122-5p as potential biomarkers of male infertility and sperm quality［J］. Reprod Biol Endocrinol， 2022，20（1）：122. doi:10.1186/s12958-022-00990-7 doi: 10.1186/s12958-022-00990-7
29	SAEBNIA N， NESHATI Z， BAHRAMI A R. Role of microRNAs in etiology of azoospermia and their application as non-invasive biomarkers in diagnosis of azoospermic patients［J］. J Gynecol Obstet Hum Reprod， 2021，50（10）：102207. doi:10.1016/j.jogoh.2021.102207 doi: 10.1016/j.jogoh.2021.102207
30	DOROSTGHOAL M， GALEHDARI H， HEMADI M， et al. Sperm miR-34c-5p Transcript Content and Its Association with Sperm Parameters in Unexplained Infertile Men［J］. Reprod Sci，2022，29（1）：84-90. doi:10.1007/s43032-021-00733-w doi: 10.1007/s43032-021-00733-w
31	ZHANG W， ZHANG Y， ZHAO M， et al. MicroRNA expression profiles in the seminal plasma of nonobstructive azoospermia patients with different histopathologic patterns［J］. Fertil Steril，2021，115（5）：1197-1211. doi:10.1016/j.fertnstert.2020.11.020 doi: 10.1016/j.fertnstert.2020.11.020

组别	年龄（岁）	体质量指数（BMI）（kg/m²）	体质量（kg）	腰围（cm）
不育组（n = 155）	28.11 ± 3.35	25.95 ± 2.26	79.89 ± 6.56	92.16 ± 8.56
正常组（n = 155）	27.95 ± 3.64	26.11 ± 2.02	80.11 ± 6.34	91.89 ± 8.76
t值	0.403	0.657	0.300	0.274
P值	0.688	0.512	0.764	0.784

精液参数	不育组（n = 155）	正常组（n = 155）	t值
精子浓度（10⁶/mL）	36.05 ± 6.25	45.98 ± 9.56	10.824
PR+NP（%）	42.56 ± 7.11	51.15 ± 6.56	11.055
PR（%）	35.25 ± 5.23	42.65 ± 7.15	10.400
精子平均曲线运动速度（μm/s）	31.15 ± 4.12	40.22 ± 5.85	15.782
精子平均直线运动速度（μm/s）	13.19 ± 2.12	19.15 ± 3.12	19.671
精子平均路径运动速度（μm/s）	15.85 ± 2.12	23.45 ± 5.12	17.075
ALH（μm）	1.62 ± 0.16	2.38 ± 0.21	35.840

组别	Zn（μmol/L）	Cu（μmol/L）	Mg（mmol/L）	Ca（mmol/L）	Fe（mmol/L）	Pb（μg/mL）	miR-184
不育组（n = 155）	8.19 ± 2.65	41.85 ± 4.05	0.87 ± 0.02	1.98 ± 0.23	8.36 ± 1.12	10.95 ± 2.11	1.25 ± 0.19
正常组（n = 155）	14.26 ± 2.35	21.56 ± 2.16	0.93 ± 0.11	2.36 ± 0.14	8.29 ± 1.22	5.46 ± 1.05	0.72 ± 0.15
t值	21.336	55.035	6.681	17.570	0.526	29.001	27.258
P值	0.000	0.000	0.000	0.000	0.599	0.000	0.000

参数	Zn	Cu	Mg	Ca	Fe	Pb
精子浓度	0.515^a	-0.581^a	0.035	0.052	-0.056	-0.161
PR+NP	0.658^a	-0.665^a	0.064	0.035	-0.048	-0.054
PR	0.634^a	-0.598^a	0.025	0.012	-0.014	-0.021
精子平均曲线运动速度	0.067	-0.025	0.089	0.056	-0.079	-0.065
精子平均直线运动速度	0.068	-0.056	0.052	0.045	-0.047	-0.025
精子平均路径运动速度	0.054	-0.032	0.065	0.089	-0.056	-0.032
ALH	0.044	-0.026	0.035	0.078	-0.041	-0.564^a

参数	miR-184
精子浓度	-0.564^a
PR+NP	-0.784^a
PR	-0.621^a
精子平均曲线运动速度	-0.645^a
精子平均直线运动速度	-0.031
精子平均路径运动速度	-0.023
ALH	-0.039

The relationship between semen quality and trace element levels in seminal plasma and miR⁃184 levels in seminal vesicles of male infertility patients

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