经后增殖方对控制性超促排卵大鼠卵巢GDF9分泌及颗粒细胞凋亡的影响

doi:10.3969/j.issn.1006-5725.2024.07.007

Abstract

Abstract:

Objective To explore the effects of Jinghou Zengzhi Granules（JHZZG）， on ovarian GDF9 secretion and granulosa cells（GCs） apoptosis in controlled ovarian hyperstimulation（COH） rats through p38MAPK/CK2/IκBα/NF-κB pathway. Methods A model of COH rats was prepared and 18 rats were randomly divided into 3 groups， including natural ovulation group（NO group）， COH group， and COH+JHZZG group. The expression of p38MAPK， CK2， IκBα， NF-κB， GDF9 mRNA and protein were detected by qRT-PCR and Western blot， and the apoptosis rate of ovarian GCs by TUNEL. Results Compared with the NO group， the expression of p38MAPK and NF-κB in the ovarian tissue of rats in the COH group increased， while the expression of CK2， IκBα and GDF9 decreased， and the apoptosis rate of ovarian GCs increased （all P < 0.01）. Compared with the COH group， the expression of p38MAPK and NF-κB in the ovarian tissue of rats in the COH+JHZZG group decreased， while the expression of CK2， IκBα and GDF9 increased （all P < 0.01）， and the apoptosis rate of ovarian GCs decreased （P < 0.05）. Conclusion Jinghou Zengzhi Granules could promote the secretion of ovarian GDF9 and inhibit the apoptosis of ovarian GCs of COH rats through p38MAPK/CK2/IκBα/NF-κB signaling pathway， which thereby improve the quality of COH oocytes.

Key words: Jinghou Zengzhi Granules, controlled ovarian hyperstimulation, p38MAPK, NF-κB, GDF9, apoptosis

CLC Number:

R271

Zhen YANG,Shaoru JIANG,Xiaoyan CHEN,Xiaolin CHEN,Weimin DENG,Xinyu. GUO. Effect of Jinghou Zengzhi Granules on ovarian GDF9 secretion and granulosa cells apoptosis in controlled ovarian hyperstimulation rats[J]. The Journal of Practical Medicine, 2024, 40(7): 918-923.

Figures/Tables 5

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References 24

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基因名称	引物序列（5′-3′）		扩增长度（bp）
r-GAPDH	Forward：	GCAAGGATACTGAGAGCAAGAG	98
r-GAPDH	Reverse：	GGATGGAATTGTGAGGGAGATG	98
r-p38MAPK	Forward：	CCAGCTAAGAAAGCTGCAAAC	101
r-p38MAPK	Reverse：	GGCTGTGTCTAATGACCCTATT	101
r-CK2	Forward：	CCGCGGACATAAAGATGAGTAG	98
r-CK2	Reverse：	GTCTTCATCCACCTCACAGAAG	98
r-IκBα	Forward：	CCTTCCTCAACTTCCAGAACAA	105
r-IκBα	Reverse：	GATCACAGCCAGCTTTCAGA	105
r-NF-κB	Forward：	CATGCGTTTCCGTTACAAGTG	111
r-NF-κB	Reverse：	CCCGTGTAGCCATTGATCTT	111
r-GDF9	Forward：	GTTCTCTCTGATAGGCGAAGTG	127
r-GDF9	Reverse：	GGTGACTTCTGCTGGGTTTA	127

组别	例数	p38MAPK	CK2	IκBα	NF-κB	GDF9
NO组	6	1.157 ± 0.206	1.248 ± 0.260	1.155 ± 0.203	0.968 ± 0.097	1.113 ± 0.134
COH组	6	4.355 ± 0.626^*	0.290 ± 0.073^*	0.605 ± 0.094^*	3.458 ± 0.383^*	0.300 ± 0.089^*
COH+JHZZG组	6	2.760 ± 0.644^*△	0.575 ± 0.086^*△	2.488 ± 0.571^*△	2.393 ± 0.323^*△	0.573 ± 0.026^*△
校正F值		75.236	45.576	43.222	148.933	70.690
P值		< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

组别	例数	凋亡率
NO组	6	0.255 ± 0.032
COH组	6	0.368 ± 0.033^*
COH+JHZZG组	6	0.266 ± 0.070^△
F值		10.107
P值		0.002

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Effect of Jinghou Zengzhi Granules on ovarian GDF9 secretion and granulosa cells apoptosis in controlled ovarian hyperstimulation rats

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