Abstract:

Objective lncRNA KCNQ1OT1 targets miR⁃129⁃5p to regulate inflammation and apoptosis in a Parkinson disease（PD）cell model. Methods Human SK⁃N⁃SH cells were cultured in vitro，and SK⁃N⁃SH was treated with 1，2，4 mmol/L MPP+ to construct a PD cell injury model. Transfect si⁃NC，si⁃lncRNA KCNQ1OT1， miR⁃NC，miR⁃129⁃5p，si⁃lncRNA KCNQ1OT1 and anti⁃miR⁃NC，si⁃lncRNA KCNQ1OT1 and anti⁃miR⁃129⁃5p into MPP+treated SK，respectively⁃N⁃SH constructs PD cells，denoted as si⁃NC+MPP+group，si⁃lncRNA KCNQ1OT1+ MPP+group，miR ⁃NC+MPP+group，miR ⁃129⁃5p+MPP+group，si ⁃lncRNA KCNQ1OT1+anti ⁃miR ⁃NC+MPP+ group，si⁃lncRNA KCNQ1OT1+anti⁃miR⁃129⁃5p+MPP+group. Flow cytometry to detect cell apoptosis；Western Blot method to detect cell Cleaved⁃caspase⁃3 protein expression；enzyme⁃linked immunosorbent assay（ELISA）to detect cell TNF⁃α，IL⁃6，IFN⁃γ levels；real⁃time Fluorescence quantitative PCR（RT⁃qPCR）was used to detect the expression of lncRNA KCNQ1OT1 and miR⁃129⁃5p in cells；the dual luciferase reporter experiment was used to detect the targeting relationship between lncRNA KCNQ1OT1 and miR ⁃129⁃5p. Results Compared with the NC group，the apoptosis rate，Cleaved⁃caspase⁃3 protein expression，TNF⁃α，IL⁃6，and IFN⁃γ levels in the 1，2， 4 mmol/L MPP+ group were significantly increased（P < 0.05）. Compared with the NC group，the expression level of lncRNA KCNQ1OT1 in the 1，2，4 mmol/L MPP+ group was significantly increased，and the expression level of miR⁃129⁃5p was significantly decreased（P < 0.05）. Compared with the si⁃NC+MPP+ group，the apoptotic rate，Cleaved ⁃caspase ⁃3 protein expression，TNF ⁃α，IL ⁃6，and IFN ⁃ γ levels in the si ⁃lncRNA KCNQ1OT1+MPP+ group were significantly reduced（P < 0.05）. Compared with the miR⁃NC+MPP+group，the apoptosis rate，Cleaved⁃ caspase⁃3 protein expression，TNF⁃α，IL⁃6，and IFN⁃γ levels in the miR⁃129⁃5p+MPP+group were significantly reduced（P < 0.05）. Compared with the si⁃lncRNA KCNQ1OT1+anti⁃miR⁃NC+MPP+group ，the si⁃lncRNA KCNQ1OT1+anti⁃miR⁃129⁃5p+MPP+group cell apoptosis rate，Cleaved⁃caspase⁃3 protein expression，TNF⁃α，IL⁃ 6. The level of IFN ⁃ γ increased significantly（P < 0.05）. Conclusion lncRNA KCNQ1OT1 down ⁃ regulates the expression of miR⁃129⁃5p to inhibit inflammation and apoptosis in PD cell models.

Key words:

lncRNA KCNQ1OT1, miR?129?5p, Parkinson′s disease, apoptosis, inflammation