慢性高脂肪饮食对缺血/再灌注大鼠脑损伤影响及机制

doi:10.3969/j.issn.1006-5725.2023.20.004

Abstract

Abstract:

Objective To evaluate the effect of chronic high fat diet （HFD） on brain injury in ischemia/reperfusion （I/R） rats and its mechanisms. Methods Sixty male Wistar rats were randomly divided into normal diet （ND） group （n = 30） and high fat diet （HFD） group （n = 30）. Each group was further divided into Sham group and I/R group， with 15 rats in each group. Neurobehavioral method was used to evaluate the neurological deficits of rats in each group； HE staining was used to detect the pathological changes of brain tissue of rats in each group； the survival rate of neurons in each group was measured by Nissl staining； western blotting method was used to detect the Pyroptosis-related proteins （NLRP3， Caspase-1， IL-1β， and HMGB1/TLR4/NF-κB signal pathway protein expression in brain tissue of rats in each group. Results Compared with the ND group， the body weight， visceral adipose tissue， triglyceride， total cholesterol and glucose water of rats in HFD group were increased （all P < 0.05）. In the I/R group， compared with the ND rats， HFD rats showed more severe neurological impairment； HE staining showed that HFD rats had more damaged cells； niss staining showed that the number of surviving neurons in HFD rats decreased； the expressions of pyroptosis related proteins （NLRP3， Caspase-1， IL-1β） were higher in HFD rats； high expression of HMGB1 and its downstream factors TLR4 and NF-κB in HFD rats （all P < 0.05）. Conclusion HFD intake could aggravate pyrodeath and enhance the expression of HMGB1/TLR4/NF-κB signaling pathway after brain I/R injury， leading to adverse outcomes after I/R.

Key words: high fat diet, ischemia/reperfusion, pyroptosis, rats

CLC Number:

R743.3

Jiashan LI,Debin YANG,Zhifeng. PENG. Effect of chronic high fat diet on brain injury in ischemia/reperfusion rats and its mechanisms[J]. The Journal of Practical Medicine, 2023, 39(20): 2579-2583.

Figures/Tables 13

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References 25

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组别	n	体质量（g）	脂肪组织（g）	甘油三酯（mg/dL）	总胆固醇（mg/dL）	葡萄糖（mg/dL）
ND组	30	398.5 ± 45.3	19.8 ± 23.4	73.8 ± 7.8	54.6 ± 5.9	134.6 ± 16.2
HFD组	30	523.6 ± 61.9	41.2 ± 5.3	96.7 ± 10.2	88.6 ± 9.7	153.8 ± 17.3
t值		26.437	39.075	14.378	31.849	11.536
P值		< 0.001	< 0.001	0.006	< 0.001	0.017

组别	n	ND	HFD	t值	P值
Sham组	15	0	0
I/R组	15	2.8 ± 0.3	3.7 ± 0.4	9.834	0.028
t值		46.936	57.423
P值		< 0.001	< 0.001

组别	n	ND	HFD	t值	P值
Sham组	5	100.0 ± 0.0	93.5 ± 9.6	4.784	0.107
I/R组	5	37.3 ± 4.2	20.6 ± 2.4	8.173	0.025
t值		63.740	75.382
P值		< 0.001	< 0.001

组别	n	ND	HFD	t值	P值
Sham组	5	100.0 ± 0.0	105.8 ± 11.2	1.946	0.832
I/R组	5	175.3 ± 18.6	227.6 ± 24.4	7.139	0.036
t值		9.164	14.826
P值		0.012	< 0.001

组别	n	ND	HFD	t值	P值
Sham组	5	100.0 ± 0.0	103.6 ± 11.3	1.238	0.896
I/R组	5	198.3 ± 20.1	247.6 ± 25.0	6.972	0.039
t值		13.836	17.649
P值		< 0.001	< 0.001

Effect of chronic high fat diet on brain injury in ischemia/reperfusion rats and its mechanisms

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组别	n	ND	HFD	t值	P值
Sham组	5	100.0 ± 0.0	108.5 ± 11.7	2.182	0.814
I/R组	5	203.3 ± 21.1	268.1 ± 28.4	6.549	0.041
t值		17.378	23.538
P值		< 0.001	< 0.001

组别	n	ND	HFD	t值	P值
Sham组	5	100.0 ± 0.0	101.5 ± 10.6	0.084	0.969
I/R组	5	217.3 ± 22.8	300.2 ± 32.4	9.152	0.013
t值		24.4280	68.354
P值		< 0.001	< 0.001

组别	n	ND	HFD	t值	P值
Sham组	5	100.0 ± 0.0	95.5 ± 9.8	1.043	0.925
I/R组	5	207.3 ± 21.4	310.3 ± 32.0	9.376	0.011
t值		22.638	74.736
P值		< 0.001	< 0.001

组别	n	ND	HFD	t值	P值
Sham组	5	100.0 ± 0.0	101.4 ± 9.8	0.028	0.987
I/R组	5	175.2 ± 19.6	283.1 ± 29.0	11.649	0.006
t值		9.543	52.457
P值		0.010	< 0.001

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