去泛素化酶组成型光形态发生因子9信号复合体5上调甲硫氨酸腺苷转移酶2A表达促进肝癌细胞增殖

doi:10.3969/j.issn.1006⁃5725.2021.12.006

The Journal of Practical Medicine ›› 2021, Vol. 37 ›› Issue (12): 1539-1543.doi: 10.3969/j.issn.1006⁃5725.2021.12.006

• Basic Research • Previous Articles Next Articles

Deubiquitinase constitutive photomorphogenic⁃9 signalosome subunit 5 upregulates the expression of methi⁃ onine adenosyltransferase 2A to promote hepatocellular carcinoma growth

XIE Peiyi，CHEN Leifeng，LU Hongcheng，DU Dongnian，LI Jiajuan，SHAO Jianghua.

Department of Hepatobiliary Surgery，Jiangxi Province Key Laboratory of Molecular Medicine，Second Affiliated Hospital of Nanchang University，Nanchang 330006，China

Online:2021-06-25 Published:2021-06-25
Contact: SHAO Jianghua E⁃mail：shao5022@163.com

Abstract

Abstract:

Objective To investigate the mechanism by which deubiquitinase constitutive photomorpho⁃ genic⁃9 signalosome subunit 5（CSN5）regulates HCC growth. Methods Through Real⁃time PCR，Western blot and Immunohistochemical staining，the expression of CSN5 and MAT2A in HCC tissues and HCC cells was detect⁃ ed. EdU assay was used to examine the proliferation capability of HCC cells. Results CSN5 was found to be signif⁃ icantly upregulated in HCC tissues and cells. CSN5 downregulation in HCC cells contributed to the cells′ inability to grow. CSN5 inhibition reduced methionine adenosyltransferase 2A expression and inhibited HCC cell prolifera⁃ tion. Conclusion CSN5 regulates HCC cells proliferation through MAT2A.

Key words:

hepatocellular carcinoma, deubiquitinase, proliferation, constitutive photomorphogenic?9 signalosome subunit 5, methionine adenosyltransferase 2A

XIE Peiyi, CHEN Leifeng, LU Hongcheng, DU Dongnian, LI Jiajuan, SHAO Jianghua. .

Deubiquitinase constitutive photomorphogenic⁃9 signalosome subunit 5 upregulates the expression of methi⁃ onine adenosyltransferase 2A to promote hepatocellular carcinoma growth [J]. The Journal of Practical Medicine, 2021, 37(12): 1539-1543.

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Deubiquitinase constitutive photomorphogenic⁃9 signalosome subunit 5 upregulates the expression of methi⁃ onine adenosyltransferase 2A to promote hepatocellular carcinoma growth

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