Abstract:

Objective To explore the relationship between the expression of miR⁃5088⁃5p in placenta and the clinical characteristics of patients with preeclampsia and its role in the pathogenesis of preeclampsia. Methods The expression of miR⁃5088⁃5p in placenta was measured by qRT⁃PCR and the correlation between the expression of miR⁃5088⁃5p and clinical features was analyzed，and the expression of LC3B and p62 in placental tissue was detected by immunofluorescence. At the same time，human placental trophoblast cell line（HTR8⁃S/Vneo）was cultured in vitro and divided into nomorxia group and hypoxia group. The expression of LC3B and p62 protein in placental trophoblasts of each group was detected by Western blot after transfection of miR⁃ 5088⁃5p mimics. Results The expression of miR⁃5088⁃5p in placenta of preeclampsia was significantly low（P < 0.01），and the expression level was negatively correlated with systolic blood pressure，diastolic blood pressure and platelet count， but positively correlated with neonatal weight（P < 0.05）. Immunofluorescence showed that LC3B increased and p62 decreased in placenta of preeclampsia group. In addition，compared with the nomorxia group，the expression of LC3BⅡ/Ⅰ protein in hypoxia group increased（P < 0.05），while the expression of p62 protein decreased（P < 0.05），and the expression of LC3BⅡ/Ⅰ decreased（P < 0.01）and p62 increased（P < 0.05）after transfection of miR⁃5088⁃5p mimics. Conclusion miR⁃5088⁃5p is significantly down⁃regulated in placental tissue of preeclamp⁃ sia，and its overexpression can significantly inhibit the occurrence of autophagy of placental trophoblasts，which is 

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