The Journal of Practical Medicine ›› 2022, Vol. 38 ›› Issue (10): 1203-1207.doi: 10.3969/j.issn.1006⁃5725.2022.10.006

• Basic Research • Previous Articles     Next Articles

RBM5 knockout in endometrial cancer cells and its effect 

WANG Saifeng，SHI Wei，XIE Xianjing.   

  1. The Inter⁃ national Peace Maternity and Child Health Hospital，School of Medicine，Shanghai Jiao Tong University，Shang⁃ hai Key Laboratory of Embryo Original Diseases，Shanghai 200030，China

  • Online:2022-05-25 Published:2022-05-25
  • Contact: SHI Wei E⁃mail：jeepgirl@126.com

Abstract:

Objective To investigate the effect of RBM5 gene knockout by CRISPR/Cas9 system on the biological behavior of endometrial cancer cells. Methods CRISPR/Cas9⁃RBM5⁃sgRNA recombinant plasmids were transfected intoRBM5 ⁃ high expression Ishikawacell lines.Western blot，qRT ⁃ PCR and Sanger sequencing were used to verify the RBM5 knockout. The abilities of proliferation，migration and invasion of parental strains，knock⁃ out strains and revertant strains were analyzed by CCK⁃8 kit，colony formation and Transwell assays. Results The expression of RBM5 was significantly decreased in the knockout cell lines（P < 0.05）. The sequencing results showed a code shift mutation. The abilities of proliferation ，migration and invasion in the RBM5⁃knockout cell significantly increased（P < 0.05）. Conclusion RBM5 knockout in endometrial cancer cell lines can promote cell proliferation，migration and invasion，which guides further molecular mechanism of endometrial cancer. 

Key words:

CRISPR/Cas9, endometrial cancer, RBM5, proliferation, migration, invasion