Abstract:

Objective To explore the expression of follicle⁃like 3（FSTL3）gene in colon cancer and its influence on the prognosis. Methods The expression of FSTL3 was detected and the clinical prognosis through the TCGA database was analyzed. The expression level of FSTL3 in colon cancer cases was detected by immuno⁃ histochemical analysis，and the relationship between its expression and the clinicopathological characteristics and prognosis was analyzed. SW620 cells were selected，cell lines with overexpression and silence of FSTL3 gene were constructed，respectively，and the transfection efficiency was detected by Western blot. MTT，clone formation test， and Transwell test were used to detect changes in proliferation and migration capabilities. Results The expression of FSTL3 in the TCGA database was significantly increased，and clinical survival analysis showed the prognosis of patients with high FSTL3 expression was significantly worse than that of patients with low FSTL3 expression（P < 0.008）. By using ROC，the evaluationof the diagnostic efficacy of FSTL3 for colon cancer was more accurate. In clinical cases，the FSTL3 gene was highly expressed（P < 0.001）. The expression of FSTL3 in colon cancer was related to age（0.049），TNM stage（P = 0.033），depth of invasion（P = 0.06），distant metastasis（P = 0.001）， and is related to gender（P = 0.386），lymphatic metastasis（P = 0.215），the degree of differentiation（P = 0.565） is irrelevant. And in colon cancer tissues，the survival rate of patients with high expression of FSTL3 was signifi⁃ cantly lower than that of patients with low expression（P < 0.008）. MTT experiments and clone formation experi⁃ ments confirmed that in SW620 cells，overexpression of FSTL3 enhanced cell proliferation，while knocking down FSTL3 decreased cell proliferation （P < 0.05）. Transwell experiments showed that overexpression of FSTL3 enhanced the migration ability of cells（P < 0.05），and knockdown of FSTL3 inhibited the migration ability of SW620 cells（P < 0.05）. Conclusion FSTL3 is highly expressed in colon cancer and is closely related to the prognosis of patients. The expression of Shen FSTL3 inhibits the proliferation and migration of colon cancer cells. FSTL3 may be considered as a molecular marker for predicting the development and prognosis of colon cancer.

Key words:

colon cancer, FSTL3, clinicopathological characteristics, proliferation, migration