The Journal of Practical Medicine ›› 2021, Vol. 37 ›› Issue (1): 25-29.doi: 10.3969/j.issn.1006⁃5725.2021.01.006

• Basic Research • Previous Articles     Next Articles

Cytotoxic effects of deubiquitinase inhibitor Au（PPh3）PT on gastric cancer cells

ZHANG Peiquan，HUANG Qingtian，LONG Huidan，LI Xiaofen
  

  1. Guangdong Provincial Key Laboratory of Protein Modification and Degrada⁃tion，Guangzhou Medical University，Guangzhou 511436，China
  • Online:2021-01-10 Published:2021-01-10
  • Contact: LI Xiaofen E⁃mail：xiaofen.li@gzhmu.edu.cn

Abstract:

Objective To investigate the effect of Deubiquitinase inhibitor Au（PPh3）PT on inducing gastric cancer cells cytotoxicity. Methods MTS assay was performed to test the cell viability of MGC⁃803 and SGC⁃ 7901 cancer cells. Apoptotic rates were assessed by microscopy and flow cytometry analysis using Annexin V/PI double staining. Apoptosis related proteins and ubiquitinated proteins were detected by western blot analysis. Results We found that Au（PPh3）PT significantly decreased the cell viability in a dose⁃ and time⁃dependent man⁃ ner. The cell apoptosis rate was obviously higher than that in control group after 24 h treatment with 1.0 μmol/L and 1.5 μmol/L of Au（PPh3）PT（P < 0.05）. It was confirmed that Au（PPh3）PT resulted in a reduction in precursor forms of caspase⁃3，⁃8，and ⁃9 with a concomitant increase in the cleaved forms of caspase⁃3，caspase⁃8，caspase⁃9， and PARP. Also，we found that a striking increase in total and K48⁃linked ubiquitinated proteins. Conclusion The results show that Au（PPh3）PT induced cytotoxicity and cell death in gastric cancer cells. This effect may be 

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