Abstract:

Objective To investigate the effect of salidroside（SAL）regulation on improving inflammation and airway remodeling associated with chronic obstructive pulmonary disease （COPD）in rats and its possible mechanism. Methods The COPD rat model was established by smoking method and randomly divided into normal group，model group，SAL low⁃dose and high⁃dose groups，positive control group. The general condition of the rats was observed，and the pulmonary function of the rats in each group was detected by the small animal pulmonary function tester；the serum inflammatory factors of the rats were detected by ELISA. The pathology of the rat lung tissue was observed by HE staining.The area of airway smooth muscle and collagen was observed by Masson staining. Western blot detection of peroxisome proliferator ⁃ activated receptor ⁃ γ（PPAR ⁃ γ）/transforming growth factor β1（TGF⁃β1）/Drosophila maternally derived skin growth factor（Smad）2/3 pathway in lung tissue Protein. Results Compared with the normal group，the levels of FEV0.1/FVC，PEF and serum IL⁃10 in the model group were decreased，and the serum IL⁃8 level was increased. There were a large number of inflammatory cells infiltration in the bronchial wall，septum and surrounding blood vessels，the alveolitis score was increased，and the smooth muscle was significantly increased. Hyperplasia and hypertrophy，significantly increased airway collagen deposi⁃ tion，increased WAm/Pbm，Wcol/Pbm values，up⁃regulated p⁃PPAR⁃γ protein expression in lung tissue，down⁃ regulated TGF⁃ β1，p⁃Smad2/3 protein expression（P < 0.05）. After the intervention of various doses of SAL， the rats′ FEV0.1/FVC，PEF value and serum IL⁃10 level increased，serum IL⁃8 level decreased，bronchial wall， septum and perivascular inflammatory cell infiltration decreased ，and alveolitis score decreased，smooth musclehyperplasia and hypertrophy improved ，airway collagen deposition decreased ，WAm/Pbm，Wcol/Pbm values decreased，p⁃PPAR⁃γ protein expression in lung tissue was down⁃regulated，TGF⁃β1，p⁃Smad2/3 protein expression was up⁃regulated（P < 0.05）. Conclusion SAL can reduce COPD⁃related inflammation and reverse airway remod⁃ eling in rats，which may be related to the regulation of PPAR⁃γ/TGF⁃β1/Smad2/3 pathway.

Key words:

salidroside, chronic obstructive pulmonary disease, inflammation, airway remodeling, mechanism of action