Abstract:

Objective To investigate the effect of SiO2 on expression of MUC5AC in Beas⁃2b and explore the related mechanism. Methods Beas⁃2b was cultured in DMEM containing 10% fetal bovine serum at 37 ℃ and 5% CO2. Beas⁃2b was transfected with lentiviruses such as NC⁃siRNA，ATG5⁃siRNA，BECN1⁃siRNA and mcherry⁃ EGFP⁃LC3. The cells in each group were transfected with corresponding lentivirus（MOI = 20）for 12 hours，then with fresh culture medium. Puromycin（1 μg/mL）was used to obtain stable transfected cell line. Cells were treated with SiO2（100 μg/mL）and PBS for 24 hours. Then protein of MUC5AC，ATG5，BECN1 and LC3 was detected by immunofluorenscent test and western blot. Results Expression of MUC5AC，ATG5，BECN1 and LC3Ⅱ in cells treated by SiO2 was signifiacntly higher than that in control cells（P < 0.05）. After stimulation of SiO2， expression of MUC5AC and LC3Ⅱ in cells transfected with ATG5⁃siRNA and BECN1⁃siRNA was lower than that in control cells（P < 0.05）. Conclusion SiO2 can enhance the expression of MUC5AC in human airway epithelial cells by promoting autophagy，which is related with the regulation of ATG5 and BECN1.

Key words: SiO2,  , autophagy,  , MUC5AC,  , ATG5,  , BECN1