Abstract:

Objective To investigate the clinical value of CD45 + CD3 + lymphocyte ratio，CD45 + CD64 + monocyte ratio，CD161+ cell ratio and 16 KD，38 KD，CFP⁃10，ESAT⁃6 and LAM tuberculosis antibody detection in the diagnosis of tuberculosis. Methods Flow cytometry was used to count the peripheral blood CD45+ mononu⁃ clear cells，CD45+ CD3+ lymphocytes，CD45+ CD64+ monocytes and CD161+ cells in 202 newly diagnosed pulmonary tuberculosis patients（tuberculosis group）and 120 healthy people（healthy group）. Protein chip technology was used to quantitatively detect the serum TB antibodies of patients with 16 KD，38 KD，CFP⁃10，ESAT⁃6 and LAM. The differences in the proportion of cells and the expression level of antibodies between the two groups were quanti⁃ tatively compared. Diagnostic test evaluation indexes were used to evaluate the clinical diagnostic performance. Results The rank sum test showed that CD45+ CD3+ lymphocytes，CD45+ CD64+ monocytes，CD161+ cells and the established tuberculosis infection risk index（RF）were significantly different between the two groups（P < 0.001）. The levels of 16 KD and LAM antibodies were significantly different between the two groups（P < 0.001）. There was no significant difference in the levels of 38 KD，CFP⁃10 and ESAT⁃6 antibodies between the two groups（P > 0.05）. The areas under the ROC curve for the diagnosis of pulmonary tuberculosis using 16 KD，38 KD，CFP10，ESAT⁃6， LAM，CD45+ CD3+ %，CD45+ CD64+ %，CD161+ % and RF were 0.748，0.525，0.564，0.535，0.680，0.860，0.834， 0.667 and 0.884. The optimal cut⁃off values were 1.031，1.074，1.075，1.062，1.161，78.480，14.250，34.765 and 0.469，respectively，and the diagnostic accuracy was 73.91%，54.04%，61.80%，51.55%，68.94%，64.29%， 79.50%，52.80% and 81.99%，respectively. Conclusion The detection of RF，CD45+ CD3+ %，CD45+ CD64+ % and 16 KD tuberculosis antibody has high clinical application value in the clinical diagnosis of pulmonary tuberculosis. 

Key words: tuberculosis,  , tuberculosis antibody,  , flow cytometry,  , diagnostic test evaluation