Abstract:

Objective To investigate the effect of miR ⁃ 191 on endometrial cancer cells and its mecha⁃ nism. Methods RT⁃PCR method was used to detect the expression of miR⁃191 and TET1 in endometrial cancer tissues，adjacent tissues and endometrial cancer cells，and the changes of TET1 mRNA were detected after transfection of miR ⁃ 191 inhibitor. MTT and Transwell method were used to detect cell proliferation and invasion ability. Western blot method was used to detect cell TET1 protein expression. The dual luciferase report analyzed the relationship between miRNA ⁃191 and TET1. Results The expression of miR ⁃191 in endometrial cancer tissues was higher than that in adjacent tissues，but the expression of TET1 was lower. Inhibition of miR⁃191 could significantly inhibit the proliferation and invasion of Ishikawa cells and up ⁃ regulate the expression level of TET1 （P＜0.05）. Dual luciferase report showed that TET1 was a potential target gene of miR⁃191，and miR⁃191 had a significant regulatory effect on the expression of TET1 gene. Conclusion miR ⁃191 may inhibit the proliferation and invasion of endometrial cancer cells by targeting down⁃regulation of TET1 expression.

Key words: