LncRNA DSCAM-AS1调节miR-150-5p/BRAF轴对甲状腺癌细胞恶性生物学行为的影响

doi:10.3969/j.issn.1006-5725.2023.23.004

Abstract

Abstract:

Objective To investigate the impact of lncRNA DSCAM-AS1 on the malignant biological behaviors of thyroid cancer （TC） cells by regulating the miR-150-5p/BRAF axis. Methods The expression of DSCAM-AS1 in TC cells was detected by qRT-PCR，and the best intervention cell line was screened.； the relationship between DSCAM-AS1， BRAF and miR-150-5p targeting regulation was verified by FISH， pull down and double Luciferase reporter gene experiment； The proliferation，migration and invasion of SW579 cells were detected； Western blot was applied to detect the expression of BRAF， E-Cadherin， and vimentin proteins； the tumor formation experiment in mice was applied to verify the effect of DSCAM-AS1 on TC tumor growth. Results DSCAM-AS1 was highly expressed in TC tissue and cells （P < 0.05）； There is a targeted regulation relationship between DSCAM-AS1，BRAF and miR-150-5p； inhibition of DSCAM-AS1 expression or overexpression of miR-150-5p obviously inhibited the proliferation， migration， invasion， and EMT of SW579 cells （P < 0.05）； Inhibition of miR-150-5p expression or overexpression of BRAF reversed the inhibitory effect of inhibition of DSCAM-AS1 expression or overexpression of miR-150-5p on the malignant behavior of SW579 cells （P < 0.05）； in vivo experiments showed that inhibiting the expression of DSCAM-AS1 obviously inhibited the growth of transplanted tumors in mice （P < 0.05）. Conclusion DSCAM-AS1 is up-regulated in TC cells， inhibiting the expression of LncRNA DSCAM-AS1 can inhibit the malignant progression of TC by regulating the miR-150-5p/BRAF signaling axis.

Key words: lncRNA DSCAM-AS1, miR-150-5p/BRAF axis, thyroid cancer, proliferation, migration, invasion

CLC Number:

R736.1

Yun PENG,Meiling WEN,Yunxia LV,Wanzhi CHEN,Chun HE,Jianping YU,Zhenluo. DING. Impact of LncRNA DSCAM⁃AS1 on the malignant biological behaviors of thyroid cancer cells by regulating the miR⁃150⁃5p/BRAF axis[J]. The Journal of Practical Medicine, 2023, 39(23): 3043-3050.

Figures/Tables 6

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Impact of LncRNA DSCAM⁃AS1 on the malignant biological behaviors of thyroid cancer cells by regulating the miR⁃150⁃5p/BRAF axis

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