Abstract:

Objective To investigate whether resveratrol regulates apoptosis of HCT 116 cells through SATB2 and its mechanism. Methods HCT 116 cells were treated with resveratrol（Res）at different concentrations for 24 h，and the optimal dose of Res was determined to be 8.33 μg/mL. HCT 116 cells were divided into control group，si⁃SATB2 group，si⁃SATB2+ Res group，empty plasmid group，SATB2 overexpression group，Res group， and SATB2 overexpression + Res group. After treatment，total protein was extracted from the cells，and the protein expressions of SATB2，Bax，Bcl2，Caspase⁃3，9 and Cyt⁃C were detected by Western Blot. Results （1）Resvera⁃ trol up ⁃ regulated the expression of SATB2 protein in HCT116 cells.（2）Knocking down of SATB2 gene incveased the Bcl⁃2/Bax ratio and down⁃reglated the expression of CYT⁃C ，Caspase⁃3 and Caspase⁃9 proteins，and Res reversed these effects.（3）Overexpression of SATB2 reduced the Bcl⁃2/Bax ratio in HCT 116 cells and down⁃regulate the expression of Cyt⁃C，Caspase⁃3 and Caspase⁃9 proteins，while Res enforced these process. Conclusion Inhi⁃ bition of SATB2 reduced the level of apoptosis in HCT 116 cells，and Res could promote the expression of apoptotic protein in HCT 116 cells by up⁃regulating SATB2.

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