GATA3介导miR-21/PTEN轴对子宫内膜癌细胞增殖、侵袭的影响

doi:10.3969/j.issn.1006-5725.2024.15.005

Abstract

Abstract:

Objective To analyze the effects of GATA binding protein 3 （GATA3） mediated mini RNA-21 （miR-21）/phosphatase and tensin homologue （PTEN） axis missing from human chromosome Chromosome 10 on the proliferation and invasion of endometrial cancer cells. Methods HEC-1-A cells were transfected and divided into control group， GATA3 empty plasmid group， GATA3 overexpression plasmid group， GATA3 siRNA negative control group， and GATA3 siRNA group. Detect the expression levels of GATA3， miR-21， PTEN， proliferation， apoptosis rate， migration， and invasion in each group of cells. Results Compared with the hEEC group， the expression levels of GATA3 and miR-21 in cells of the HEC-1-A group， HEC-1-B group， and Ishikawa group increased， while the expression levels of PTEN decreased （P < 0.05）. Compared with the GATA3 empty plasmid group， the GATA3 overexpression plasmid group showed an increase in GATA3， miR-21 mRNA expression， proliferation rate， migration distance， number of invading cells， and Vimentin levels， while the PTEN mRNA expression， apoptosis rate， Caspase-9， Bax， and E-cadherin levels decreased （P < 0.05）； Compared with the GATA3 siRNA negative control group， the GATA3， miR-21 mRNA expression， proliferation rate， migration distance， number of invading cells， and Vimentin level decreased， while the PTEN mRNA expression， apoptosis rate， Caspase-9， Bax， and E-cadherin levels increased （P < 0.05）. Conclusion Downregulation of GATA3 expression can regulate the miR-21/PTEN axis， slow down the proliferation of HEC-1-A cells， and promote apoptosis of HEC-1-A cells.

Key words: endometrial carcinoma, GATA binding protein 3, microRNA-21, phosphatase and tensin homologues of human chromosome 10 deletion, proliferate, invasion

CLC Number:

R735.2

Fahui WANG,Qingchun DENG,Jiajia LIN,Chunfei. CHEN. GATA3 mediates the effect of miR⁃21/PTEN axis on the proliferation and invasion of endometrial cancer cells[J]. The Journal of Practical Medicine, 2024, 40(15): 2069-2074.

Figures/Tables 7

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References 25

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组别	GATA3	miR-21	PTEN
hEEC	1.01 ± 0.21	1.00 ± 0.01	1.00 ± 0.01
HEC-1-A组	2.48 ± 0.36	3.25 ± 0.41	0.52 ± 0.06
HEC-1-B组	2.15 ± 0.31	3.01 ± 0.32	0.81 ± 0.09
Ishikawa组	2.32 ± 0.33	3.13 ± 0.36	0.73 ± 0.08
F值	10.590	18.700	10.590
P值	0.001	0.001	0.001

组别	GATA3 mRNA	miR-21 mRNA	PTEN mRNA
对照组	1.00 ± 0.01	1.00 ± 0.01	1.00 ± 0.01
GATA3空载质粒组	1.02 ± 0.02	0.97 ± 0.01	0.95 ± 0.01
GATA3过表达质粒组	3.25 ± 0.42	4.52 ± 0.53	0.28 ± 0.02
GATA3 siRNA阴性对照组	0.99 ± 0.01	1.02 ± 0.02	1.01 ± 0.03
GATA3 siRNA组	0.25 ± 0.03	0.32 ± 0.04	4.25 ± 0.53
F值	75.000	52.150	19.390
P值	0.001	0.001	0.001

组别	WT-GATA3	MUT-GATA3
miR-NC组	1.52 ± 0.23	0.93 ± 0.09
miR-21组	1.96 ± 0.28	0.95 ± 0.08
t值	3.840	0.606
P值	0.001	0.441

组别	增殖率	凋亡率
对照组	100.00 ± 10.00	9.18 ± 0.42
GATA3空载质粒组	98.52 ± 9.93	9.22 ± 0.44
GATA3过表达质粒组	175.63 ± 23.14	3.52 ± 4.52
GATA3 siRNA阴性对照组	99.62 ± 10.25	9.15 ± 0.40
GATA3 siRNA组	52.63 ± 6.41	52.36 ± 7.25
F值	12.610	18.800
P值	0.001	0.001

组别	迁移距离（μm）	侵袭细胞数（个）
对照组	152.63 ± 26.36	76.52 ± 8.33
GATA3空载质粒组	150.25 ± 25.09	77.02 ± 8.45
GATA3过表达质粒组	248.25 ± 36.31	196.63 ± 24.02
GATA3 siRNA阴性对照组	153.52 ± 26.44	75.02 ± 8.27
GATA3 siRNA组	85.63 ± 9.42	12.05 ± 2.63
F值	7.569	23.340
P值	0.001	0.001

GATA3 mediates the effect of miR⁃21/PTEN axis on the proliferation and invasion of endometrial cancer cells

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组别	Caspase-9	Bax	E-cadherin	Vimentin
对照组	0.85 ± 0.09	0.71 ± 0.08	0.67 ± 0.07	0.60 ± 0.07
GATA3空载质粒组	0.86 ± 0.10	0.69 ± 0.07	0.65 ± 0.06	0.59 ± 0.06
GATA3过表达质粒组	0.28 ± 0.03	0.22 ± 0.03	0.11 ± 0.02	1.62 ± 0.24
GATA3 siRNA阴性对照组	0.87 ± 0.11	0.72 ± 0.09	0.69 ± 0.08	0.61 ± 0.08
GATA3 siRNA组	1.85 ± 0.24	2.38 ± 0.32	1.75 ± 0.08	0.11 ± 0.02
F值	12.340	16.010	32.130	21.280
P值	0.001	0.001	0.001	0.001

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