LncRNA SNHG8在胎盘植入的表达及其对滋养细胞侵袭及迁移的影响

doi:10.3969/j.issn.1006-5725.2024.05.010

Abstract

Abstract:

Objective To investigate the expression of lncRNA SNHG8 in placenta accrete （PA） and its effect on trophoblast invasion and migration. Methods qRT-PCR was used to detect the expression of lncRNA SNHG8 in placenta tissue of 30 cases in PA group and 30 cases in control group， and the correlation between lncRNA SNHG8 expression and prenatal ultrasound score of 30 cases in PA group was analyzed. Transwell and scratch assay were used to detect the effect of lncRNA SNHG8 interference on the invasion and migration of human chorionic trophoblast cells （HTR8/SVneo cells）， and western blot was used to detect the expression of MMP-2 and MMP-9. The downstream targets of lncRNA SNHG8 were predicted by StarBase software， and the expression of lncRNA SNHG8 was detected in placental tissues of the two groups. Dual luciferase reporter assay was used to detect the targeting relationship between lncRNA SNHG8 and miR-542-3p. Results Compared with that of the control group， the expression of lncRNA SNHG8 was up-regulated in the placenta tissue of the PA group（P < 0.05）， and it was positively correlated with prenatal ultrasound score. Interference with lncRNA SNHG8 inhibited the invasion and migration of trophoblast cells（P < 0.05）； the protein expression of MMP-9 and MMP-2 also decreased significantly （P < 0.05）. Biological prediction indicates that miR-542-3p had a binding site with lncRNA SNHG8， and miR-542-3p expression was down-regulated in PA placental tissue（P < 0.05）. Dual luciferase reporter assay confirmed that lncRNA SNHG8 could target miR-542-3p. Compared with si-SNHG8+inhibitor-NC， co-transfection of si-SNHG8 and miR-542-3p inhibitor enhanced the invasion and migration ability of trophoblast cells（P < 0.05）. Conclusion lncRNA SNHG8 is highly expressed in PA and is related to the severity of PA. LncRNA SNHG8 promotes the invasion and migration of trophoblast by regulating the level of miR-542-3p. The study suggests that lncRNA SNHG8 plays an important role in the invasion and migration of PA trophoblast cells， which is expected to be a clinical diagnostic biomarker and therapeutic target.

Key words: lncRNA SNHG8, miR-542-3p, placenta accreta, invasion, migration

CLC Number:

R714.25

Li′na GAO,Xiaohui LIU,Yufang ZHANG,Xiaoling LIU,Xiaochun HE,Jing GAO,Li ZHANG,Jun SUN,Xiujuan WANG,Yan. DONG. Expression of lncRNA SNHG8 in placenta accreta and its effect on trophoblast invasion and migration[J]. The Journal of Practical Medicine, 2024, 40(5): 646-652.

Figures/Tables 8

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References 25

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临床资料	PC组	PA组	t值	P值
年龄（岁）	30.83 ± 0.83	31.9 ± 0.70	0.97	0.330
孕周（周）	38.93 ± 0.11	37.01 ± 0.08	13.41	< 0.001
孕次	2.40 ± 0.14	3.60 ± 0.15	5.26	< 0.001
产次	1.03 ± 0.10	1.53 ± 0.10	3.43	< 0.001
剖宫产次数	0.90 ± 0.11	1.43 ± 0.10	3.51	< 0.001

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Expression of lncRNA SNHG8 in placenta accreta and its effect on trophoblast invasion and migration

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