Abstract:

Objective The same concentration of growth factor has different effects on cell proliferation and the aim of this study was to find the optimal concentrations of PDGF⁃BB and VEGF⁃A for HUVEC proliferation. Methods Lentivirus GFP was transfected into HUVECs and PDGF⁃BB and VEGF⁃A were grouped in concentra⁃ tions：0 μg/mL GFs（Control），0.02 μg/mL GFs，0.2 μg/mL GFs，2 μg/mL GFs，5 μg/mL GFs. GFP⁃HUVEC was inoculated into the porous plate or gel and cultured for a certain time. Then the proliferation of GFP⁃HUVEC was detected by Fluorescence microscope or CCK⁃8. Results （1）HUVEC transfected with GFP was in good condi⁃ tion and showed strong fluorescence under microscope.（2）HUVEC was cultured with different concentrations of growth factors in the hole plate. The cells had the highest proliferative activity at 2 μg/mL PDGF⁃BB（P < 0.001） and 0.02 μg/mL VEGF⁃A（P < 0.000 1），respectively.（3）CCK⁃8 showed the highest cell proliferative activity in 2 μg/mL PDGF⁃BB（P < 0.05）and 0.02 μg/mL VEGF⁃A（P < 0.001）.（4）The cells were encapsulated in the hy⁃ drogel and cultured in the medium with different concentrations of growth factors. The cells had the highest prolifer⁃ ative activity at 2 μg/mL PDGF ⁃BB（P < 0.01）and 0.02 μg/mL VEGF ⁃A（P < 0.01），respectively. Conclusion HUVECs respectively had the best effect of cell proliferation in 2 μg/mL PDGF⁃BB and 0.02 μg/mL VEGF⁃A. How⁃ ever，high concentrations of growth factors may have an inhibitory effect on them.

Key words: growth factors,  , concentration,  , cell proliferation,  , culture environment