The Journal of Practical Medicine ›› 2021, Vol. 37 ›› Issue (13): 1670-1673.doi: 10.3969/j.issn.1006⁃5725.2021.13.005

• Basic Research • Previous Articles     Next Articles

miR ⁃ 209 inhibits proliferation of glioma U87 and enhances its radiosensitivity by downregulating signal pathway of MEK/ERK 

杨彬，于晶， 刘晓智， 陈镭    

  1. Department of Neurosurgery，Tianjin Fifth Central Hospital，Tianjin 300450，China 
  • Online:2021-07-10 Published:2021-07-10

Abstract:

Objective To investigate the effect of miR⁃209 down regulating the MEK/ERK signal pathway on the proliferation and radiosensitivity of glioma U87 cells. Methods The expressions of miR ⁃209，MEK and ERK mRNA in U87 cells before and after radiotherapy were detected by Real Time PCR. After miR⁃209 transfection， the expressions of miR⁃209，ERK and MEK were detected by Western blot and real time PCR. Results After radiotherapy，the relative expression of miR⁃209 in U87 cells decreased（t = 2.491，P = 0.0374），and there was no significant difference in ERK and ERK mRNA expressions between pre⁃ and post⁃radiotherapy. After the miR⁃209 was transfected to the U87 cells，the MEK mRNA expression decreased（0.11 ± 0.05），The ERK mRNA expression decreased（F = 9.891，P = 0.002 9），and the p⁃MEK and p⁃ERK expressions both decreased，and cell prolifera⁃ tion was inhibited. Conclusion miR⁃209 can inhibit cell proliferation and enhance radiosensitivity by down regu⁃ lating the expression of MEK⁃ERK in U87 cells.

Key words:

glioma, radiosensitivity, signal pathway