脂肪酸结合蛋白5结合Vimentin蛋白在肝癌中的作用机制

doi:10.3969/j.issn.1006-5725.2024.06.005

Abstract

Abstract:

Objective The aim of this study was to screen and verify the proteins interacting with Vimentin， investigate the regulatory relationship between FABP5 and candidate proteins， and further explore the mechanism of FABP5 in hepatocellular carcinoma. Methods Immunoprecipitation combined with tandem mass spectrometry （IP?MS） was used to screen the proteins that bind to FABP5. The binding relationship between FABP5 and candidate interacting proteins was verified from the exogenous and endogenous levels by Co?immune precipitation assay （Co?IP）. RT?qPCR， Western blot and immunofluorescence were used to observe the effect of knockdown FABP5 on the transcription and translation of Vimentin in HCC cells. The effect of overexpressing FABP5 on the cytoskeleton of HCC cell was observed by phalloidin staining. Results 336 potential target proteins that bind to FABP5 were identified through IP?MS. Based on literature， five candidate proteins related to tumors were selected， namely PRDX1， PRSS3， PKM， HSP90AA1， and Vimentin. The binding relationship between FABP5 and Vimentin protein was confirmed through both exogenous and endogenous Co?IP. Knockdown FABP5 has no significant effect on the expression of Vimentin mRNA， but it can inhibit the expression of Vimentin protein， and overexpression of FABP5 can affect the cytoskeleton of HCC cell. Conclusions FABP5 promotes the migration and invasion of HCC cells by the regulation of Vimentin and the influence of cytoskeletal remodeling， and thus it is expected to be a potential target for anti?HCC and provide new ideas for the treatment of HCC.

Key words: fatty acid binding protein 5, Vimentin, liver cancer, mass spectrometry, interacting protein

CLC Number:

R735.7

Yanping TANG,Kezhi LI,Zhengmin CAI,Hao TAO,Jiaying TANG,Xueyu LI,Yanjuan LI,Ji CAO. Study on the mechanism of fatty acid binding protein 5 binding Vimentin protein in the Hepatocellular Carcinoma cells[J]. The Journal of Practical Medicine, 2024, 40(6): 756-761.

Figures/Tables 4

References 25

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Study on the mechanism of fatty acid binding protein 5 binding Vimentin protein in the Hepatocellular Carcinoma cells

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