Abstract:

Objective To investigate the effect of pirinixic acid（WY⁃14643），the peroxidase proliferator⁃activated receptor⁃alpha agonist（PPAR⁃α agonist），on oxidative stress and apoptosis of parahepatic tissues in livercancer after transcatheter arterial embolization（TAE）. Methods VX2 liver cancer model with 60 rabbits weresuccessfully established，the rabbits randomly divided into three groups：the control，TAE and combined treatment（WT），20 in each. The TAE group was treated with TAE operation，the WT group with injection of WY⁃14643［3 mg/（kg·d）］through the ear vein for 3 consecutive days before TAE，and the control group did not receive anytreatment. After operation，4ml of peripheral blood was drawn from the ear vein for examinations of liver functionindicators ALT and AST，followed by detection of oxidative stress indicators SOD，GSH⁃Px，CAT and MDA usingchemiluminescence，detection of apoptosis of the liver cells and calculation of the apoptotic index （AI）usingTUNEL，and detection of the mRNA and protein expressions of SOD1，Bcl⁃2 and caspase⁃3 using RT⁃qPCR andWestern blot. Results The liver function indexes，MDA content and expressions of AI and Caspase⁃3 in the TAEgroup were significantly increased，and the SOD，GSH⁃Px，CAT activity and expressions of SOD1 and Bcl⁃2 inthe TAE group were significantly decreased. The data in the WT group were opposite the TAE group（P < 0.05）.Conclusion PPAR⁃ α agonist pirinixic acid can inhibit oxidative stress by enhancing the activity of antioxidantenzymes SOD，GSH ⁃Px and CAT and enhancing the expressions of SOD1，reduce cell apoptosis by upregulatingthe expression of Bcl⁃2 and downregulating the expression of Caspase⁃3，so that the damage to liver tissues adja⁃cent to cancer after TAE can be mitigated.

Key words:

liver cancer, transcatheter arterial embolization, WY ?14643, peroxisome proliferatorsactivated receptor?α, oxidative stress, apoptosis