The Journal of Practical Medicine ›› 2023, Vol. 39 ›› Issue (3): 298-308.doi: 10.3969/j.issn.1006⁃5725.2023.03.006

• Basic Research • Previous Articles     Next Articles

Effects of cinnamaldehyde on proliferation,EMT and stem cell characteristics of PC⁃3 cells

WU Junxi*, TANG Yubo,LINGHU Xitao,BAO Guanglong,SHI Haoran,WEN Zhenyu,HUANG Shuai,WA Qingde.   

  1. Depart⁃ ment of Orthopedics,the Second Affiliated Hospital of Zunyi Medical University,Zunyi 563000,China 

  • Online:2023-02-10 Published:2023-02-10
  • Contact: WA Qingde E⁃mail:wqd887zsy@126.com

Abstract:

Objective To explore the effects of cinnamaldehyde(CA)on the proliferation,epithelial ⁃ mesenchymal transition(EMT)and stem cell characteristics of PC⁃3 cells and their mechanisms,so as to provide reference for preliminary experiment of CA as a potential drug preventing bone metastasis in prostate cancer. Methods The effects of CA on the proliferation,migration,invasion,adhesion and spheres formation of PC⁃3 cells were detected using CCK8,Transwell,matrix adhesion and spheres formation assays. The expressions of miR⁃143 and AGO2 mRNA in PC⁃3 cells after CA treatment were determined by qRT⁃PCR,while the effects on the invasion, migration and adhesion of PC⁃3 cells in the single CA,single transfection of miR⁃143mimic or AGO2⁃siRNA, simultaneous treatment of CA and AGO2,or miR ⁃143 mimic and AGO2 group were detected by Transwell and matrix adhesion assays. Furthermore,the protein expressions of CA or transfection of miR⁃143 mimic on EMT and stem cell characteristics of PC⁃3 cells were determined using western blot. Results CA inhibited the proliferation, migration,invasion,adhesion and spheroid of PC⁃3 cells in a concentration⁃dependent manner,showing statistically significant differences while compared with that in the control group(< 0.01). qRT⁃PCR showed that CA up⁃reg⁃ ulated the expression of miR⁃143 and down⁃regulate the expression of AGO2 mRNA in PC⁃3 cells. Overexpression of miR⁃143 or down⁃regulation of AGO2 inhibited the migration,invasion and adhesion of PC⁃3 cells. Also,the inhibition on the invasion,migration and adhesion of PC⁃3 cells was not obvious after the simultaneous up⁃regulation of AGO2 and CA or miR⁃143 and AGO2. In addition,CA and overexpression of miR⁃143 inhibited the expressions of AGO2,ZEB1,vimentin,N⁃cadherin,fibronectin,CD44,Oct⁃4 and c⁃Myc proteins in PC⁃3 cells. Conclusion CA inhibits the proliferation,migration,invasion and adhesion of PC⁃3 cells in vitro;it also inhibits the EMT and stem cell characteristics of PC⁃3 cells,and its mechanism may be related to its ability to up⁃regulate the miR⁃143/ AGO2 axis.

Key words:

cinnamaldehyde, PC?3, miR?143, EMT, stem cell characteristics ,