With the aggravation of social aging，the morbidity and mortality of heart failure are gradually increasing. Therefore，more and more attention has been paid to diverse therapies for the treatment of heart failure all over the world. At the same time，new progress has been made in the treatment of heart failure，and the guidelines for heart failure have also been constantly updated in many countries. At present，angiotensin receptor enkephalin inhibitors，sodium and glucose cotransporter 2 inhibitors，soluble guanylatecyclase activators，iron supplements， recombinant human relaxin⁃2 and cardiac rehabilitation have been proved to benefit patients with heart failure. This article will review the progress in the treatment of heart failure in order to provide reference for clinical treatment.

Acute clinical events and adverse prognosis of thromboembolic diseases seriously threaten patients′ life and quality of life. The development of thrombolytic technique has greatly reduced the incidence of thromboembolic events. Compared with traditional drug thrombolysis，surgical thrombectomy and intraductal throm⁃ bolysis，ultrasound combined with targeted microbubble thrombolytic technology has the advantages of wide throm⁃ bolytic time window and accurate thrombolytic drug dose，as well as the thrombolytic effect is significant and easy to evaluate. This article focuses on the preparation and characteristics of targeted microbubbles，the mechanism of ultrasound ⁃assisted thrombolysis mediated by targeted microbubbles，and the research progress of targeted micro⁃ bubbles in experimental and clinical application. 

Objective To observe the effect of sacubitril valsartan on ventricular remodeling in rabbits with chronic heart failure and explore its underlying mechanisms. Methods Rabbit models of chronic heart failure were established in the heart failure group and sacubitril valsartan group. The sacubitril valsartan group was given sacubitril valsartan（10 mg/kg）intragastric administration；the sham operation group and the heart failure group the equivalent amount of normal saline. Echocardiography and N⁃terminal pro⁃brain natriuretic peptide（NT⁃proBNP） in serum were detected，and western blot was performed to evaluate the expression level of signaling pathway related proteins，as well as the protein expression level of α⁃ SMA，ColⅠ，ColⅢ，MMP2，MMP9，TIMP1，TGF ⁃β， Smad2，Smad3，Smad6 and Smad7. Results Compared with the heart failure group，the protein level of α⁃SMA， ColⅠ，ColⅢ，MMP2，MMP9，TGF⁃β，Smad2 and Smad3 in the sacubitril valsartan group decreased，while the protein expression level of Smad6 and Smad7 increased. Conclusion Sacubitril valsartan can affect collagen fiber deposition and degradation，reduce myocardial fibrosis and improve myocardial remodeling by regulating the TGFβ/ Smad signaling pathway.

Objective To observe the effect ofubiquitin binding enzyme e2t（UBE2T）geneinfection on the proliferation of human ovarian cancer cell line SKOV3，and to explore its mechanism. Methods UBE2T shRNA and lentiviral vector carrying UBE2T and NC ⁃ shRNA genes were constructed and transfected into human ovarian cancer cell line SKOV3，andwere recorded as silencing group，transfection group and negative control group， respectively. The untransfected human ovarian cancer cell line SKOV3 was taken and recorded as the blank control group. Three double wells were set in every group to make cell suspension，and the cell concentration was adjusted to 1 × 105 pieces/mL，culture for 72 h. Results The cells in the silence group were significantly reduced，with loose connection and morphological changes. The inhibition rate of cell proliferation and the proportion of G1 phase cells in the silence group were higher than those in the other three groups（P < 0.05），which in the transfection group were lower than those in the negative control group and blank control group（P < 0.05），and the percentages of cells in S phase and G2 phase，the mRNA and protein expressions of UBE2T，AKT，Cyclin D1，phosphorylated Akt（p⁃AKT）were lower than those in the other three groups（P < 0.05），which in the transfection group were higher than those in the negative control group and blank control group（P < 0.05）. Conclusion The down⁃regulation of UBE2T expression couldinhibit the proliferation of human ovarian cancer cell line SKOV3，while the overexpres⁃ sion of UBE2T gene couldpromote the proliferation of human ovarian cancer cell line SKOV3.

Objective To investigate the effects of baicalin on proliferation，invasion and epithelial⁃mes⁃ enchymal transformation（EMT）of cervical cancer SiHa cells，and whether these effects can be achieved through microrNA（miR ⁃410）/Janus kinase 2（JAK2）/signal transduction and transcription activator 3（STAT3）. Methods SiHa cells were treated with different concentrations of baicalin. MTT assay，flow cytometry and Transwell assay were used to detect the cell survival rate，apoptoticrate，and cell invasion ability，so was Western blot to detect the relative expression levels of epithelial cadherin（E⁃cad），neurocadherin（N⁃cad）and Vimentin related to epithelial mesenchymal transformation. SiHa cells were randomly divided into acontrol group，NC group，miR⁃410 inhibitor group and inhibitor plus baicalin group. miR⁃410 level was detected by qRT⁃PCR，and the relative protein expres⁃ sion levels of p ⁃JAK2 and p ⁃STAT3 were detected by Western blot. Results The survival rate，invasion ability and relative expression levels of N⁃cad and Vimentin protein in SiHa cells decreased gradually with an increase inbaicalin concentration，while the apoptotic rate and relative expression levels of E⁃cad protein increased gradually with a rise inbaicalin concentration（P < 0.05）. As compared with the miR⁃410 inhibitor group，the relative protein expression levels of p⁃JAK2 and p⁃stat3 were decreased in the inhibitor plusbaicalein group（P < 0.05）. Conclusions Baicalein can inhibit proliferation，invasion and EMT of cervical cancer SiHa cells，possibly by regulating themiR⁃ 410 /JAK2/STAT3 signaling axis.

Objective To explore the mechanism of action of lncRNA H19（H19）/Sirt3 mediated autoph⁃ agy in injury after intracerebral hemorrhage（ICH）. Methods Amouse model of ICH was established by injections with collagenase. SH⁃SY5Y cells treated with oxyhemoglobin（OxyHb）were used as an in vitro ICH model. Alenti⁃ viral vector containing sh⁃H19 was used to inhibit H19 expressions in ICH mice and cultured SH⁃SY5Y cells. 48 hours after establishment of the ICH model，the mouse neurologic deficits were analyzed by mNSS score and lesion volume. RT⁃qPCR，Tunel staining and immunofluorescence were used to analyze the expression of H19，neuronal apoptosis and LC3 express in the injured tissues respectively. Cell viability was measured by MTT assay，apoptosis was detected by Tunel staining，and expressions of autophagy ⁃ related proteins and Sirt3/AMPK/mTOR signaling pathway were analyzed by Western blot. Results The expression of H19 was increased after ICH and in OxyHb⁃ treated cells in a time⁃dependent manner（P < 0.05）. H19 knockdown reduced the lesion volume and mNSS score on the second day after establishment of the ICH model（P < 0.01），decreased neuronal apoptosis（P < 0.01）， and enhanced regulation of autophagy during ICH injury. H19 knockdown enhanced the viability of SH⁃SY5Y cells exposed to OxyHb，reduced apoptosis，and accelerated autophagy. H19 accelerated autophagy mainly through regu⁃ lating Sirt3 expression and activating AMPK⁃mTOR pathway. Conclusions H19 is enhanced in a time⁃dependent manner in ICH models in vivo and in vitro. H19 knockdown relieves ICH damage via autophagy induced by Sirt3/ AMPK/mTOR signaling pathway.

Objective To explore the potential mechanism of Helicobacter pylori（H. pylori）metabolites in regulating the secretion of inflammatory factors. Methods After infection with E. coli（E.Coli）or H. pylori，the secretion level of key inflammatory factors IL⁃33，TNF⁃α and IFN⁃γ in human gastric cancer cells AGS was detected. Twenty⁃four hours after H.pylori infection of AGS cells，the cell supernatant was collected for liquid chromatogra⁃ phy and tandem mass spectrometry to detect H. pylori metabolites，and key metabolites were identified by screen⁃ ing. Results After E.Coli or H. pylori infection，the secretion level of IL⁃33，TNF⁃α and IFN⁃ γ in AGS cells increased. Compared with E. coli infection，AGS cells infected with H. pylori secreted lower level of IL⁃33，TNF⁃α， and IFN⁃γ. The H. pylori metabolite adenosine decreased the secretion level of IL⁃33，TNF⁃α and IFN⁃γ in a gradi⁃ ent⁃dependent manner. Adenosine level of AGS cells were not significantly changed after infection with adenosine synthase knockout strain H. pyloriΔAdA strain. After H. pyloriΔAdA infection，the secretion level of IL⁃33，TNF⁃α，and IFN⁃ γ in AGS cells was higher than that in H. pyloriWT⁃infected AGS cells. After knockdown of A2A receptor， the secretion level of IL⁃33，TNF⁃ α，and IFN⁃ γ increased in H. pylori⁃infected AGS cells. Four weeks after H. pyloriΔAdA or wild⁃type H. pyloriWT infection of mice，the level of IL⁃33，TNF⁃α，and IFN⁃γ in the serum of mice all increased. The level of IL⁃33，TNF⁃α and IFN⁃ γ in the serum of mice after H. pylori Δ AdA infection increased significantly. In addition ，the titers of anti ⁃H.pylori antibodies produced in mice after H. pylori Δ AdA infection increased. Conclusion Adenosine synthase expressed by H. pylori can promote the production of adenosine，and then adenosine regulates the secretion of inflammatory factors of IL⁃33，TNF⁃α and IFN⁃γ through A2A receptors， and reduces the level of adaptive immunity.

Objective The aim of this study was to analyzed the CRRT factors of acute Stanford type A aortic dissection surgery retrospectively and identify the risk factors for CRRT after acute Stanford type A aortic dissection surgery. Methods 115 patients（93 males and 22 females，at a mean age of（53.16 ± 11.43）years with acute Stanford type A aortic dissection at the cardiovascular surgery department of our hospital from September 2020 to December 2021）were collected and retrospectively analyzed. 23 patients were acute kidney injury after re⁃ ceive acute Stanford type A aortic dissection surgery were assigned into the CRRT group，and the remaining pa⁃ tients（n = 92）were included in the non⁃CRRT group. The perioperative data were reviewed and the risk factors were identified by univariate analysis，which further confirmed by logistic regression. Results There were 19 pa⁃ tients dead during perioperative period in our study. There were 9 deaths in the CRRT group and 10 deaths in the non⁃CRRT group. The mortality of the two groups was statistically significant（χ2 = 10.655，P = 0.001）. Univariate analysis identified Preoperative creatinine，block time，extracorporeal circulation time，operation time，intraopera⁃ tive red blood cell volume，intraoperative plasma volume were risk factors for CRRT after acute Stanford type A aor⁃ tic dissection surgery. Logistic regression analysis further identified preoperative creatinine （OR = 1.875，P = 0.014），blocking time（OR = 1.874，P = 0.008），time of operation（OR = 1.014，P = 0.012）and intraoperative erythrocyte transfusion（OR = 2.183，P = 0.017）were independent risk factors for CRRT after acute Stanford type A aortic dissection surgery. The receiver operating characteristic（ROC）curve analysis showed that preoperative creatinine was the most effective in predicting postoperative CRRT of acute Stanford type A aortic dissection.Conclusion It was confirmed that preoperative creatinine，block time，CPB time and intraoperative red blood cell volume were independent risk factors for CRRT after acute Stanford Type A aortic dissection. Effective perioperative assessment and management of patients can reduce the risk of CRRT after acute Stanford type A aortic dissection.

Objective The study aimed to compare the difference and clinical significance of Xanthine oxidase and uric acid in heart failure（HF）patients with different ejection fraction. Methods A total of 120 HF patients were enrolled and divided into HFrEF group，HFmrEF group and HFpEF group，with 40 patients in each group respectively. Another 40 patients without heart failure but other types of cardiovascular diseases were selected as the control group. The clinical data were collected. The levels of uric acid（UA）and xanthine oxidase（XO） were determined by Enzyme Linked Immunosorbent Assay（ELISA）. Results Compared with the control group， XO and UA levels in the three groups were all statistically increased（F = 60.257，F = 19.024，both P < 0.01）. Spearman correlation analysis showed that serum XO and UA levels in patients with heart failure were positively correlated with NT⁃proBNP（r = 0.571，r = 0.404）and negatively correlated with LVEF（r = -0.185，r = -0.270）， with statistically significant differences（all P < 0.05）. Multivariate regression analysis showed that the risk factors for heart failure with different ejection fraction included XO and UA. In addition，history of coronary heart disease was a risk factor for heart failure with reduced ejection fraction，and history of atrial fibrillation was a risk factor for heart failure with retained ejection fraction. Conclusion XO and UA are risk factors for patients with heart failure. They have certain reference value for clinical diagnosis of patients with heart failure and may even be an important index to evaluate the degree of impairment of heart function in patients with heart failure.

Objective To explore the influential factors of left ventricular reverse remod⁃eling（LVRR）in patients with reduced ejection fraction heart failure after ST⁃segment eleva⁃tion myocardial infarction. Methods A total of 108 patients who were confirmed with STEMI and had LVEF less than 40% after primary percutaneous coronary intervention（PCI）were enrolled. According to the results of echocardiogram indicators after six months， all patients were divided into LVRR group and NLVRR group. The routine baseline and operation data collected through the electronic medical record system were compared between the two groups. The multivariate Logistic regression model was used to explore the influential factors of LVRR. The receiver operating curve（ROC curve） was used to analyze the predictive values of related factors. Results Compared with NLVRR group，the prevalence of peak NT⁃proBNP，peak cTnT，IBil，LVEDV，LVESV，TIT were lower，while FS，complete revascularization were higher in LVRR group（P < 0.05）. Regression analysis found the influential factors of LVRR were peak cTnT， TIT and complete revascularization（P < 0.05）. Peak cTnT and TIT were risk factors for LVRR，while complete revascularization was protective factor. ROC curve analysis showed the AUC of TIT combined with peak cTnT detec⁃ tion was higher than peak cTnT or TIT alone（AUC were 0.817，0.714 and 0.737，P < 0.05）. Conclusion Peak cTnT，TIT and complete revascularization were independent influential factors of LVRR in patient with HFrEF after STEMI. Peak cTnT and TIT were risk factors for LVRR，while complete revascularization was protective factor. The predictive value of peak cTnT combined with TIT for LVRR was higher than that of peak cTnT or TIT alone.

Objective To investigate the prognostic value of prognostic nutritional index（PNI）in patients with hepatitis B virus related chronic and acute liver failure（HBV⁃ACLF）. Methods Clinical data of 82 patients with non⁃liver transplantation HBV⁃ACLF treated in Nantong Third People′s Hospital from January 2019 to Novem⁃ ber 2021 were collected. They were divided into survival group and death group according to 90⁃day mortality after admission. Risk factors affecting the prognosis of patients were analyzed by binary logistic regression；The clinical efficacy of PNI，MELD score，MELD⁃Na score，iMELD score and PTA were analyzed by ROC curve；The survival curve was drawn by Kaplan Meier method. Results There was no significant difference in sex，age，HBeAg status，complications，artificial liver treatment and HBV⁃DNA between the two groups（P > 0.05）. PNI，albumin， PTA and lymphocytes in the survival group were significantly higher than those in the death group（P < 0.05）. MELD score，MELD Na score and iMELD score in the death group were higher than those in the survival group （P < 0.05），and the difference was statistically significant（P < 0.05）. Multivariate analysis showed that PNI was a prognostic factor in patients with liver failure（P < 0.05）. Pearson correlation analysis showed that PNI was negatively correlated with MELD score，MELD⁃Na score and iMELD score，but positively with PTA（all P < 0.05）. ROC curve analysis showed that the area under the curve of PNI was higher than that of MELD score，MELD Na score，imeld score and PTA. Kaplan Meier analysis showed that the cumulative survival rate of patients in low PNI group was significantly lower than that in high PNI group（P < 0.05）. Conclusion PNI is helpful to evaluate the severity of HBV⁃ACLF patients and is an important parameter for prognosis.

Objective To evaluate the clinical effect of protective rehabilitation intervention for pulmonary ventilation function in patients undergoing laparoscopic hiatal hernia repair complicated with GRED. Methods Sixty⁃ eight patients undergoing laparoscopic hiatal hernia repair（LHHR）complicated with GRED who were admitted to our hospital from August 2017 to March 2019 were selected as the research object. They were randomly divided into protection group and volume group，with 34 in each group. The mean arterial pressure（MAP），central venous pressure（CVP），pulmonary compliance（C），alveolar⁃arterial differential blood oxygen pressure（A⁃ADO2）， end ⁃expiratory partial pressure of carbon dioxide（PETCO2），oxygenation index（OI），and pulmonary compliance （C）were observed and recorded before pneumoperitoneum（T0），2 h（T1），4 h（T2）and 24 h（T3）after pneumo⁃ peritoneum in the 2 groups. The incidence of heart rate（HR），respiratory rate（RR）and related complications， combined with the length of postoperative hospital stay，were evaluated for the postoperative rehabilitation effect. Results Compared with those before pneumoperitoneum（T0），postoperative MAP and CVP were significantly increased，while C was significantly decreased（P < 0.05）. At T1 and T2，the decrease of C in protection group was greater than that in volumn group（P < 0.05）. Compared with those before pneumoperitoneum，a⁃ADO2 and PETCO2 were significantly decreased in patients（P < 0.05）. At T1，T2 and T3，the decrease of A⁃ADO2 and PETCO2 in the protection group was greater than that in the capacity group（P < 0.05）. Compared with those before pneumoperito⁃ neum，OI and RR were significantly increased，and HR in the protection group was significantly decreased at T1 and T2（P < 0.05），while the change of HR in the volume group at T1，T2 and T3 was insignificant when compared with that before pneumoperitoneum（P > 0.05）. At T1，T2 and T3，OI and RR of the protection group increased more than those of the volume group（P < 0.05）. There were lower cumulative incidence of related complications andless hospitalization days in the protection group（P < 0.05）. Conclusion Protective rehabilitation intervention is conducive to the recovery of pulmonary ventilation in patients undergoing LHHR complicated with GRED，which is worthy of promotion.

Objective To investigate the analgesic effect of multi⁃mode percutaneous electrical nerve stim⁃ ulation（TENS）combined with multi ⁃mode analgesia of enhanced recovery after surgery（ERAS）in laparoscopic total hysterectomy. Methods Sixty patients who planned to undergo laparoscopic total hysterectomy were randomly divided into ERAS group（n=30）and ERAS + TENS group（n = 30）. Visual analogue scale（VAS）and Ramsay sedation score were compared between two groups at 4 h，8 h，16 h and 24 h after surgery. Serum β⁃endorphin lev⁃ els were detected before anesthesia induction，30 min after operation，and when patients were awake after surgery. The amount of intraoperative blood loss，operation time，intraoperative anesthetic dosage，nausea and vomiting in postoperative 48 h，postoperative exhaust time and postoperative hospital stay were compared between the two groups. Results Compared with ERAS group，the VAS score decreased at 16 h after surgery，and Ramsay seda⁃ tion score increased at 8 h and 16 h after surgery in ERAS+TENS group（P < 0.05）. The levels of β⁃endorphin in ERAS+TENS group were significantly higher than those in ERAS+TENS group at 30 min after operation and at post⁃ operative wakefulness（P < 0.05）. There were no significant differences in intraoperative blood loss，operation du⁃ ration，postoperative hospital stay，postoperative exhaust time，dosage of propofol and remifentanil，and incidence of nausea and vomiting between the two groups（P > 0.05）. But the incidence of non⁃incision pain was significantly decreased in ERAS + TENS group（P < 0.05）. Conclusion TENS combined with ERAS multi ⁃mode analgesia could reduce postoperative pain and improve sedation comfort in patients with laparoscopic total hysterectomy，but could not reduce the amount of opioids.

Objective To analyze the curative effect and short ⁃ term effect of percutaneous mechanical thrombectomy（PMT）in patients with acute lower extremity arterial ischemia. Methods A retrospective analysis was performed on 74 patients with lower extremity arterial ischemia who were admitted to our department from Janu⁃ ary 2019 to July 2021. Among them，40 were treated with Anjiojet mechanical thrombus aspiration system（PMT group），and 34 with catheter ⁃ directed thrombolysis（CDT group）. The dosage of urokinase，thrombolysis time， hospitalization time，hospitalization cost，postoperative complications，and the incidence of patency and mortality within one month after surgery were compared between the two groups，and 36⁃item short⁃form health survey（SF⁃36） was used to compare the quality of life between CDT group and PMT group. Results The effective rate of surgical treatment in both groups was 100% . The dosage of urokinase in the CDT group and PMT group was（137.41 ± 47.02）× 104 U and（58.35 ± 39.83）× 104 U；thrombolysis time was（35.85 ± 11.60）h and（22.30 ± 11.82）h； hospitalization time was（10.31 ± 2.9）days and（8.55 ± 2.11）days；hospitalization expenses were（3.32 ± 1.12） ten thousand yuan and（5.22 ± 0.89）ten thousand yuan and the incidence of bleeding and puncture bleeding were 17.6% and 2.5%，respectively. SF⁃36 showed that the score of body pain was（53.86 ± 13.89）and（63.02 ± 10.39）， and that of emotional function（58.04 ± 10.32）and（63.61 ± 13.19）respectively in the two consecutive days after the operation，and the differences were statistically significant（P < 0.05）. There was no significant difference in patency rate（88.2% vs. 90.0%）and fatality rate（3.0% vs. 5.0%）within one month（P > 0.05）. Conclusion In the treatment of acute lower extremity arterial ischemia，AngioJet can significantly reduce postoperative bleeding and puncture point hematoma，shorten hospitalization time，and reduce operation cost while ensuring the same efficacy as CDT，and improve the quality of life of patients after operation.

Objective To investigate the effect of ulinastatin combined with Shenmai Injection on myocar⁃ dial injury protection and pulmonary function in patients with severe pneumonia. Methods One hundred and twenty patients with severe pneumonia were randomly divided into control group and observation group，with 60 cases in each group. The control group was treated with Shenmai injection，and the observation group was treated with ulina⁃ statinand for Shenmai injection 2 consecutive weeks. The clinical efficacy，inflammatory factors，myocardial indexes， blood gas indexes and Apache⁃Ⅱ score were compared between the two groups. Results After treatment，the total effective rate of the observation group was higher than that of the control group（P < 0.05）. Compared with those before treatment，FEV1% and FEV1/FVC in the two groups increased after treatment，and FEV1/FVC in the observation group was higher than that in the control group（all P < 0.05），but there was no significant difference between the two groups（P > 0.05）. After treatment，TNF⁃α，CRP and PCT were reduced in the two groups，which were lower in the observation group than those in the control group（P < 0.05）；CK ⁃MB，LDH，α⁃HBDE，NT ⁃ proBNP and cTnⅠin the observation group were lower than those in the control group（all P < 0.05）；PaO2，SaO2 and OI in the two groups were higher than those before treatment，which were higher in the observation group（all P < 0.05）；the Apache Ⅱ score of the two groups was lower than that before treatment，and that of the observation group was lower than that of the control group（all P < 0.05）. The incidence of adverse reactions such as vascular pain，diarrhea and skin pruritus in the observation group was significantly lower than that in the control group （all P < 0.05）. Conclusion Shenmai injection combined with ulinastatin can significantly reduce the pulmonary inflammatory state of patients with severe pneumonia，improve blood gas and protect damaged myocardium. It has good curative effect and less adverse reactions.

Objective To investigate the effect of Ganshuang granule combined with entecavir on plasma D⁃dimer level in patients with hepatitis B related cirrhosis. Methods Patients with hepatitis B related cirrhosis with elevated D ⁃ dimer were randomly divided into two groups：The observation group （191 cases） took the Ganshuang granule 0.3 grams/time，3 times/day，Entecavir 0.5 mg/time，1 time/day of 48 weeks. 165 cases in the control group were treated with entecavir 0.5 mg/time，once a day for 48 weeks. The changes of plasma D⁃dimer levels at baseline，6 months and 12 months of treatment were compared by repeated measurement analysis of variance. Results the plasma D⁃dimer of the two groups was 3.44 ± 1.33 mg/L and 3.59 ± 1.30 mg/L at baseline （P > 0.05）. Repeated measurement ANOVA showed that the main effect between groups was significant，F = 58.953，P < 0.001，partial η2 = 0.143；The main effect of time was significant，F = 545.167，P < 0.001，partial η2 = 0.606；The interaction effect between time and group was significant，F = 10.117，P < 0.001，partial η2 = 0.028. At 6 and 12 months after treatment，the decrease of plasma D⁃dimer level in the observation group was higher than that in the control group（P < 0.05）；The level of plasma D⁃dimer decreased at baseline，6 months and 12 months in each group（P < 0.05）. There were significant differences in the incidence of portal vein thrombosis， ascites and gastrointestinal bleeding between the two groups（P < 0.05）. Conclusion Compared with entecavir alone，Ganshuang granule combined with entecavir could significantly reduce the level of plasma D⁃dimer in patients with hepatitis B cirrhosis，and the effect was more obvious with the extension of time，which could effectively prevent the formation of portal vein thrombosis.

Objective To investigate the clinical effect and safety of vonolaxan combined with amoxicillin and clarithromycin for seven⁃day eradication of Helicobacter pylori（Hp）. Methods A total of 200 patients with Hp infection diagnosed in Dongguan Songshan Lake Tungwah Hospital from June 2021 to December 2021 were selected and randomly divided into a study group of 100 cases and a control group of 100 cases. The study group received Vonolaxan combined with amoxicillin and clarithromycin triple therapy for seven days ，while the control group received standard quadruple therapy for 2 weeks. All patients received carbon 13 breath test again after at least 4 weeks of drug. The general data，adverse reactions，Hp eradication rate and cost of the two groups of patients were compared. Results The number of cases in the study group and control group were 18 and 17 due to loss to follow⁃up and drop out of the study，respectively. In the end，82 samples in the study group and 83 samples in the control group were obtained. There was no statistical difference in the general data of age and gender between the two groups（P > 0.05）. In terms of adverse reactions，the overall incidence rate of the study group was 7.3%， compared with 12.0% of the control group，the difference was not statistically significant（χ2 = 1.054，P = 0.305）. In the study group，the eradication rate of Hp was 74.0%（74/100）by intention to treat（ITT）analysis and 90.2% （74/82）by per protocol（PP）analysis. In the control group，the eradication rate of Hp was 72.0%（72/100）by ITT analysis and 86.7%（72/83）by PP analysis. There was no significant difference between the two groups in the eradication rate of Hp by ITT（χ2 = 0.101，P = 0.750）and PP analysis（χ2 = 0.495，P = 0.482）（P > 0.05）. Conclusions Vonolaxan combined with amoxicillin and clarithromycin triple therapy for seven days is effective and safe in eradicating Hp，which is worthy of widespread clinical promotion.

Objective To investigate the diagnostic value of serum miR ⁃ 134 and miR ⁃301 level in liver fibrosis in patients with chronic hepatitis C（CHC）. Methods One hundred and fifty patients with CHC admitted to our hospital were selected as CHC group，and 150 healthy people as control group during the same period. The level of ALT，AST，TBIL，miR⁃134 and miR⁃301 was compared between the two groups. The CHC group was divided into different subgroups according to the liver fibrosis stage（S0 ⁃ S4 grade），and the level of various indexes among subgroups were compared. The ROC curve was used to analyze the clinical value of ALT，AST，TBIL，miR⁃134 and miR⁃301 level in the diagnosis of significant liver fibrosis（S2⁃S4 stage）. Results Compared with that in the control group，the level of ALT，AST，TBIL，miR⁃134 and miR⁃301 in the CHC group increased significantly（P < 0.05）. As the stage of liver fibrosis increased，the level of ALT，AST，TBIL，miR⁃134 and miR⁃301 increased（P < 0.05）. ROC curve results showed that the AUC of ALT，AST，and TBIL in the diagnosis of significant liver fibrosis was < 0.7，while the AUC of miR ⁃ 134 and miR ⁃ 301 level in the diagnosis of significant hepatic fibrosis > 0.70. Conclusion The serum level of miR⁃134 and miR⁃301 in patients with CHC increases significantly，and is closely related to the degree of liver fibrosis in patients. The detection of miR⁃134 and miR⁃301 has certain clinical value in the diagnosis of significant liver fibrosis.

Objective To investigate the relationship between metabolic syndrome （MetS）and its compo⁃ nents and the risk of female ovarian cancer. Methods As the research object，a prospective study included 311 750 cases of employees and retirees who underwent team physical examinations in the health management center of Sichuan Provincial People′s Hospital from January 2014 to December 2016.The baseline sociodemo⁃ graphic characteristics were collected through questionnaire survey，and the obesity，blood pressure，blood glu⁃ cose MetS and its components，such as blood lipid，were followed up every year. Multivariate Cox proportional hazards regression model was used to analyze the relationship between MetS and its components and the risk of female ovarian cancer. Results Ovarian cancer was diagnosed in 350 new cases，with an overall incidence den⁃ sity of 20.41/100000 person years. Mets patients had a higher risk of ovarian cancer than non Mets patients ［aRR（95%CI）：1.436（1.048 ~ 1.969），P = 0.002］；Among the abnormal components of MetS，overweight and / or obese people have a higher risk of ovarian cancer than those with normal BMI［aRR（95%CI）is 1.220 （1.025 ~ 1.453），P = 0.001］，and dyslipidemia people have a higher risk of ovarian cancer than those with normal blood lipid［aRR（95%CI）is 1.305（1.077 ~ 1.581），P = 0.004］. The results of stratified analysis showed that young people（≤50 years old）［RR（95%CI）was 3.219（1.900 ~ 5.453），P<0.001］，smoking/ passive smoking［RR（95%CI）was 2.155（1.202 ~ 3.865），P = 0.010］，non⁃smoking/quitting［RR（95%CI） was 1.800（1.038 ~ 3.123），P = 0.036］，drinking［RR（95%CI）was 1.984（1.178 ~ 3.341），P = 0.013］， non⁃drinking / quitting［RR（95%CI）was 1.933（1.059 ~ 3.528），P = 0.034］，No physical exercise［RR（95%CI）was 2.745（1.391 ~ 5.420），P = 0.001］，mental stress was very high / relatively high［RR（95%CI）was 1.770（1.018 ~ 3.076），P = 0.018］. Conclusion Overweight and/or obesity，dyslipidemia，and MetS can all increase a woman′s risk of ovarian cancer. As a result，controlling weight and maintaining normal BMI and blood lipid levels through lifestyle intervention measures is an effective primary preventive measure to reduce the risk of ovarian cancer in Chinese women.