内质网应激蛋白葡萄糖调节蛋白78在哮喘炎症和严重程度评估中价值

doi:10.3969/j.issn.1006-5725.2026.04.003

Abstract

Abstract:

Objective To assess the potential of GRP78 as a clinical biomarker in asthma by evaluating its relationship with key inflammatory cytokines and pulmonary function. Methods A total of 115 asthma patients （including 57 with mild， 47 with moderate， and 11 with severe asthma） and 42 healthy controls were enrolled. Serum levels of GRP78， IL-4， IL-13， IL-17， and IFN-γ were measured using ELISA. Pulmonary function parameters， including FEV1% and FEV1/FVC%， were also collected for comprehensive analysis. Results The level of serum GRP78 in the asthma patient group was significantly higher than that in the normal control group （P < 0.01）， and it increased with the severity of the disease. The levels of GRP78 in the mild， moderate and severe asthma groups were （23.42 ± 0.12） ng/mL，（28.01 ± 0.03） ng/mL and （33.33 ± 0.14） ng/mL respectively， all of which were higher than that in the control group （16.38 ± 0.11） ng/mL （P < 0.05）. The levels of serum IL-4， IL-13 and IL-17 in asthma patients ［（2 754.07 ± 7.32） pg/mL，（2 490.72 ± 41.32） pg/mL，（7 080.38 ± 546.32） pg/mL respectively］ were significantly higher than those in the normal control group ［（1 493.44 ± 344.32） pg/mL，（1 280.18 ± 20.43） pg/mL，（3 668.92 ± 321.51） pg/mL respectively］（P < 0.01）， while the level of IFN-γ ［（652.90 ± 42.67） pg/mL］ was lower than that in the control group ［（1 101.3 ± 111.42） pg/mL］（P < 0.01）. Pearson correlation analysis showed that GRP78 was positively correlated with the levels of IL-13 and IL-17 （IL-13： r = 0.34， P < 0.05； IL-17： r = 0.26， P < 0.05）， and negatively correlated with the levels of IFN-γ， FEV1% and FEV1/FVC% （IFN-γ： r = -0.31， P < 0.05； FEV1%： r = -0.21， P < 0.05； FEV1/FVC%： r = -0.32， P < 0.05）. Conclusions Serum GRP78 is elevated in asthma and correlates with disease severity， Th2/Th17 inflammation， and impaired lung function. These findings support its potential utility as a clinically relevant biomarker for asthma evaluation and monitoring.

Key words: asthma, GRP78, Th2 cytokines, Th17 cytokines, Th1 cytokines

CLC Number:

R562.2⁺5

Xiangli FENG,Kecheng YAN,Xiaoli FENG,Juan GE,Yuanyuan WU. Endoplasmic reticulum stress protein GRP78 in asthma inflammation and severity assessment[J]. The Journal of Practical Medicine, 2026, 42(4): 559-563.

Figures/Tables 6

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References 26

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组别	例数	年龄/岁	性别/例		BMI/（kg/m2）
组别	例数	年龄/岁	男	女	BMI/（kg/m2）
哮喘组	115	45.12 ± 12.21	66	49	22.35 ± 1.34
正常对照组	42	43.31 ± 10.24	25	17	24.18 ± 2.43
t/χ2值		0.610	0.253		0.362
P值		0.405	0.205		0.351

组别	例数	FEV₁%	FEV₁/FVC%
哮喘组	115	75.63 ± 4.46	64.54 ± 4.31
正常对照组	42	88.65 ± 5.83	78.43 ± 5.32
t值		12.31	14.12
P值		< 0.01	< 0.01

Endoplasmic reticulum stress protein GRP78 in asthma inflammation and severity assessment

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组别	例数	IL-4	IL-13	IL-17	IFN-γ
哮喘组	115	2 754.07 ± 7.32	2 490.72 ± 41.32	7 080.38 ± 546.32	652.90 ± 42.67
正常对照组	42	1 493.44 ± 344.32	1 280.18 ± 20.43	3 668.92 ± 321.51	1 101.3 ± 111.42
t值		22.342	34.242	23.456	14.341
P值		< 0.01	< 0.01	< 0.01	< 0.01

组别	例数	GRP78
对照组	42	16.38 ± 0.11
轻度	57	23.42 ± 0.12
中度	47	28.01 ± 0.03
重度	11	33.33 ± 0.14
F值		12.05
P值		< 0.05

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