m6A修饰在良性卵巢相关疾病的研究进展

doi:10.3969/j.issn.1006-5725.2024.16.024

Abstract

Abstract:

N6-methyladenine （m6A） modification is involved in a variety of biological processes and dynamically regulates RNA stability. When m6A modification is changed， it affects the development of follicles， granulosa cell proliferation and oocyte development， and participates in the occurrence and development of benign ovaria-related diseases， such as polycystic ovary syndrome， premature ovarian dysfunction， and endometriosis， resulting in decreased female fertility. This article reviews the role and research progress of m6A modification in benign ovaria-related diseases， aiming to provide new diagnosis and treatment ideas for subsequent research on improving fertility.

Key words: N6-methyladenine, benign ovarian disease, polycystic ovary syndrome, premature ovarian insufficiency, endometriosis

CLC Number:

R711.5

Yanzhi WU,Yang. LIU. Research progress of m6A modification in benign ovarian related diseases[J]. The Journal of Practical Medicine, 2024, 40(16): 2352-2356.

Figures/Tables 3

Fig.1

Tab.1

Tab.2

References 44

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检测技术		优点	缺点
定量检测技术	比色法^［14］	操作简单，方便快捷，灵敏度高	无特异性
	斑点印迹法^［15］	时间周期短，成本低	半定量形式
	HPLC-MS/MS^［16］	精准，敏感度和特异度高，样本量大	样品前处理繁琐，分析时间长
	SCAELET^［17］	-	步骤复杂、实验周期长使用放射性试剂
定位检测技术	MeB-RIP-seq^［18］	样品起始量低，全转录组信息覆盖	重复性低，分辨率低
定位检测技术	mi CllP-seq^［19］	-	依赖抗体，灵敏性和重复性、准确性相对较差
定量定位检测技术	m6A-SAC-seq^［20］	检测样本多样，重复性好，精度高，样本量要求低	-
定量定位检测技术	m6A-REF-seq^［21］	不依赖抗体，精准检测	不能覆盖所有位点

		机制
PCOS	甲基化酶METTL3	缺陷METTL3，可能导致细胞凋亡^［24］
	去甲基化酶FTO	FTO表达上调，导致GCs障碍^［26］
	阅读蛋白YTHDF1	YTHDF1表达降低，改善PCOS症状^［27］
POI	甲基化酶METTL3	METTL3表达上调，导致POF^［30］
	阅读蛋白YTHDF2	致病变异与POI发生有关^［31］
	甲基化酶KIAA1429	颗粒细胞凋亡，可能造成卵泡闭锁^［32］
	去甲基化酶ALKBH5和FTO	表达下调，导致POF^［33-34］
EMS	甲基化酶METTL3	表达上调，抑制ESCs的增殖^［42］
	甲基化酶METTL14	表达下调，促进ESCs增殖和入侵^［43］
	阅读蛋白YTHDF2	促进ESC的细胞衰老^［42］
	去甲基化酶FTO	上调FTO，促进子ESCs增殖^［44］

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Research progress of m6A modification in benign ovarian related diseases

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