Abstract:

Objective To investigate the effects of Schisandrin B （SchB） on myocardial function and apoptosis in Hyperthyroidism heart disease （HHD） rats， and to provide theoretical basis for the mechanism of SchB's intervention on myocardial apoptosis and myocardial function. Methods Hyperthyroidism model group and SchB drug intervention group were established into HHD rat model， and SchB drug intervention group was given SchB thinner ［80 mg/（kg·d）］. Velocity Vector imaging Technology （VVI） was used to analyze each group. Serum TT3， TT4 and NF-κB levels were detected by ELISA. Apoptosis of cardiomyocytes was observed by TUNEL staining. Western blot was used to explore the expression of samples' NF-κB. The tissue cells of left ventricular samples were observed by electron microscope. Results Compared with CON group， peak systolic and diastolic velocities （Vs， Vd）， peak strain （Smax） and peak systolic and diastolic strain rates （sSRr， dSRr） in HHD group were decreased （P < 0.01）； The serum TT3 （P < 0.01）， TT4 （P < 0.01）， NT-proBNP （P < 0.05） and NF-κB （P < 0.01） concentrations increased. The number of TUNEL positive particles increased significantly. The expression of NF-κB was increased by Western blotting （P < 0.05）.The structure of myocardial cells was disordered and apoptosis was obvious under electron microscope. Compared with HHD group， Vs （P < 0.01）， Vd （P < 0.05）， Smax （P < 0.01）， sSRr （P < 0.05） and dSRr （P < 0.05） were increased in HHD+SC group； The serum TT3 （P < 0.05）， TT4 （P < 0.05）， NT-proBNP （P < 0.05） and NF-κB （P < 0.01） levels were decreased. The number of TUNEL positive particles decreased significantly. The expression of NF-κB was decreased by Western blot （P < 0.05）. Under electron microscope， the structure of myocardial cells was more orderly and the apoptosis was reduced. Conclusion SchB can ameliorate HHD myocardial injury and inhibit apoptosis of myocardial cells.

Key words: velocity vector imaging, NF-κB, schisandrin B, hyperthyroidism heart disease, cell apoptosis