Abstract:

Objective To analyze the regulation of gene expression by adenosine deaminase RNA specific 1（ADAR1） in cervical cancer cell line Hela using RNA-seq technology and provide theoretical basis for understanding the role of ADAR1 in the occurrence and progression of cervical cancer. Methods RNA-seq sequencing of normal and ADAR1 knockdown Hela cell lines to identify differentially expressed genes. By conducting enrichment analysis using KEGG Pathway， GO cellular， and GSEA， the study analyzes the relevant signaling pathways and biological processes involving ADAR1 in Hela cell lines. Results Differentially expressed genes are mainly enriched in immune and inflammation-related signaling pathways （such as TNF-α/NF-κB， NIK/NF-κB， Jak/Stat-IL-6）， Hippo signaling pathway， TGF-β signaling pathway， and are involved in interferon response， cellular amino acid metabolism regulation， protein ubiquitination/deubiquitination， viral transcription， and other biological processes. Further analysis of the NF-κB signaling pathway revealed a significant increase in the mRNA expression levels of NF-κB1 and TRAF5 after ADAR1 knockdown. Conclusion ADAR1 may regulate the expression of NF-κB signaling pathway-related factors and thereby regulate the occurrence and development of cervical cancer.

Key words: adenosine deaminase RNA specific 1, cervical cancer, RNA-seq technology, NF-κB signaling pathway