血清DCLK1、sTim-3对前列腺癌根治术患者预后的评估价值

doi:10.3969/j.issn.1006-5725.2023.18.014

Abstract

Abstract:

Objective To investigate the prognosis of serum bicorticotin-like kinase 1 （DCLK1） and soluble T cell immunoglobulin mucin molecule 3 （sTim-3） in patients undergoing radical prostatectomy. Methods This study selected 80 patients who underwent radical prostatectomy in our hospital from June 2016 to June 2019 as the research group， and another 80 healthy individuals who underwent physical examination during the same period as the control group； enzyme linked immunosorbent assay （ELISA） was applied to detect serum levels of DCLK1 and sTim-3； The relationship between serum DCLK1 and sTim-3 and pathologic features and prognosis of patients undergoing radical prostatectomy was analyzed. Results The serum levels of DCLK1 and sTim-3 in the study group were obviously higher than those in the control group （P < 0.05）. Serum DCLK1 and sTim-3 levels were positively correlated （P < 0.05）. Both were positively correlated with PSA level and Gleason score （P < 0.05）.Serum DCLK1 and sTim-3 expression levels were associated with PSA levels， Gleason scores， and TNM stages （P < 0.05）. The serum levels of DCLK1 and sTim-3 in the prognostic death group were significantly higher than those in the prognostic survival group （P < 0.05）. Patients with high expression of DCLK1 and sTim-3 had lower survival rates than those with low expression. According to Cox regression analysis， high expression of DCLK1 and sTim-3 was a risk factor affecting the prognosis of patients undergoing radical prostatectomy （P < 0.05）. According to the ROC curve， the AUC that jointly predicted the prognosis of patients undergoing radical prostatectomy was better than that of DCLK1 and sTim-3 respectively. Conclusion DCLK1 and sTim-3 are obviously elevated in the serum of prostate cancer patients， and their combination can better evaluate the prognosis of patients undergoing radical prostatectomy.

Key words: double cortin like kinase 1, soluble T cell immunoglobulin and mucin-domain containing molecule-3, radical prostatectomy for prostate cancer, prognosis

CLC Number:

R737.25

Minyu HUANG,Jun WU,Tianzi QIN,Wei ZHOU,Lize SU,Rong QIU,Lu. HUANG. The prognostic value of serum DCLK1 and sTim⁃3 in patients undergoing radical prostatectomy for prostate cancer[J]. The Journal of Practical Medicine, 2023, 39(18): 2368-2372.

Figures/Tables 8

Tab.2

Tab.3

Tab.4

Fig.1

Fig.2

Tab.5

Tab.6

Fig.3

References 25

1	张鲁东，崔丽丽，马勇，等. 前列腺癌根治术后病理提示神经周围侵犯的危险因素及其对术后复发的影响［J］. 临床尿外科杂志， 2018， 33（3）： 238-241.
2	丁琪，孙慕斌，金晓华，等. 减瘤性前列腺癌根治术联合内分泌治疗在寡转移前列腺癌患者中的疗效及预后观察［J］. 实用医学杂志，2023，39（4）：460-464.
3	刘俊，董永超，徐东波，等. 前列腺癌患者预后影响因素的研究进展［J］. 实用医学杂志，2021，37（10）：1365-1370.
4	WU Q， CHEN G， QIU S， et al. Target-triggered and self-cal-ibration aptasensor based on SERS for precise detection of a prostate cancer biomarker in human blood［J］. Nanoscale， 2021， 13（16）： 7574-7582.
5	孙旭东，乔庆东，王磊，等. 血清VEGF、PSA水平与前列腺癌根治术后短期预后的相关性分析［J］. 医学理论与实践， 2022， 35（1）： 118-120.
6	LAWAL I O， BRUCHERTSEIFER F， VORSTER M， et al.Prostate-specific mem-brane antigen-targeted endoradiotherapy in metastatie prostate cancer［J］. Curr Opin Urol， 2020， 30（1）： 98-105.
7	MAKINO S， TAKAHASHI H， OKUZAKI D， et al. DCLK1 inte-grates induction of TRIB3， EMT， drug resistance and poor prog-nosis in colorectal cancer ［J］. Carcinogenesis， 2020， 41 （3）：394-396.
8	BAILLY C， THURU X， GOOSSENS L， et al. Soluble TIM-3 as a biomarker of progression and therapeutic response in cancers and other of human diseases［J］. Biochem Pharmacol， 2023， 209（1）： 115445-115454.
9	BITON J， OUAKRIM H， DECHARTRES A， et al. Impaired tumor-in-filtrating t cells in patients with chronic obstructive pulmonary disease impact lung cancer response to PD-1 blockade［J］.Am J Respir Crit Care Med， 2018， 198（7）：928-940.
10	中华医学会泌尿外科学分会前列腺癌联盟. 中国前列腺癌早期诊断专家共识［J］. 中华泌尿外科杂志， 2015， 36（8）： 561-564.
11	蒋仕春，高顺，马跃，等. PI3K/p110β和PTEN在前列腺癌中的表达及意义［J］. 生物技术， 2021， 31（5）：465-468，512.
12	段凯军，李青. P504s、CK-HMW、p63、S-100蛋白与前列腺癌病理分级的关系［J］. 实用中西医结合临床， 2021， 21（4）：12-14，49.
13	LI J， WANG Y， GE J， et al. Doublecortin-Like Kinase l（DCLK1） regulates B cell-specifie moloney murine leukemia virus ingertion site 1 （Bmi-1） and is aseociated with metastasis and prognosis in pancreatic cancer［J］. Cell Physiol Biochem， 2018， 51（1）：262-277.
14	周美玲，贺兼斌，向明，等. DCI.K1和A20在非小细胞肺癌中的表达及意义［J］. 临床肺科杂志， 2018， 23（8）：1464-1467.
15	陈贤真，乔建瓯. 血清中DCLK1在非小细胞肺癌中的表达及应用价值［J］. 中国实验诊断学， 2019， 23（11）： 1924-1928.
16	李伟枫，林铁成，谢咏，等. 血清miR-15b， DCL.K1水平与宫颈癌患者临床病理特征及预后的关系［J］.山东医药， 2021， 61（28）：19-22.
17	韩蕃颉，王海滨，苏新云，等. 3项血清肿瘤标志物、SAA及DCLK1联合检测在非小细胞癌患者预后判断中的价值［J］. 国际检验医学杂志， 2021， 42（17）： 2154-2157.
18	向慧敏，李筱，饶琴琴，等. 宫颈癌患者血清双皮质素样激酶1、黏蛋白-1、血管生成素-2的表达及临床意义［J］. 检验医学与临床， 2023， 20（5）： 655-659.
19	CHEN B J， DASHNAMOORTHY R， GALERA P， et al. The immunecheckpoint molecules PD-1， PD-L1， TIM-3 and LAC-3in diffuse large B-cell lymphoma［J］. Oncotarget， 2019，10 （21）：2030-2040.
20	代娇，宋秋玲，段慧英，等. 活动性肺结核患者Tim-3/Galectin-9的表达变化及其与巨噬细胞相关细胞因子的相关性［J］. 实用医学杂志， 2019， 35（7）： 1101-1105.
21	饶亚华，冯先华，晏黎，等. 乳腺癌患者血清可溶性T细胞免疫球蛋白黏蛋白分子-3与其配体的表达及临床意义［J］. 肿瘤学杂志， 2021， 27（9）： 742-746.
22	SOLINAS C， DE SILVA PBRON D，et al. Significance of TIM3expression in cancer；from biology to the clinie［J］. Semin Omcol，2019，46（4-5）；372-379.
23	熊斌，何秀珍，叶小兰，等. 血清可溶性Tim-3预测肝细胞癌动脉化疗栓塞治疗的疗效和预后的价值［J］. 临床肿瘤学杂志， 2022， 27（6）： 527-532
24	PENG Y， ZHANG C， RUI Z， et al. A comprehensive profiling of soluble immune checkpoints from the sera of patients with non‐small cell lung cancer［J］. J Clin Lab Anal， 2022， 36（2）： e24224.
25	CONG Y， CUI Y， ZHU S，et al. Tim-3 promotes cell aggres-siveness and paclitaxel resistance through NF-KB/STAT3signalling pathway in breast cancer cells［J］. Chin J Cancer Res， 2020， 32（5）： 564- 579.

指标	DCLK1		sTim-3
指标	r值	P值	r值	P值
PSA水平	0.503	< 0.001	0.510	< 0.001
Gleason评分	0.497	< 0.001	0.494	< 0.001
DCLK1	-	-	0.491	< 0.001
sTim-3	0.491	< 0.001	-	-

病理学特征	例数	DCLK1		χ² 值	P值	sTim-3		χ² 值	P值
病理学特征	例数	高表达（n = 42）	低表达（n = 38）	χ² 值	P值	高表达（n = 41）	低表达（n = 39）	χ² 值	P值
年龄				2.677	0.102			0.865	0.352
> 60岁	35	22（62.86）	13（37.14）			20（57.14）	15（42.86）
≤ 60岁	45	20（44.44）	25（55.56）			21（46.67）	24（53.33）
PSA水平				6.960	0.008			5.263	0.022
> 20 ng/mL	24	18（75.00）	6（25.00）			17（70.83）	7（29.17）
≤ 20 ng/mL	56	24（42.86）	32（57.14）			24（42.86）	32（57.14）
Gleason评分				6.189	0.013			9.725	0.002
> 7分	28	20（71.43）	8（28.57）			21（75.00）	7（25.00）
≤ 7分	52	22（42.31）	30（57.69）			20（38.46）	32（61.54）
肿瘤直径				1.011	0.315			0.075	0.784
> 3.0 cm	32	19（59.38）	13（40.63）			17（53.13）	15（46.88）
≤ 3.0 cm	48	23（47.92）	25（52.08）			24（50.00）	24（50.00）
TNM分期				7.234	0.007			5.714	0.017
T₁ - T₂期	51	21（41.18）	30（58.82）			21（41.18）	30（58.82）
T₃ - T₄期	29	21（72.41）	8（27.59）			20（68.97）	9（31.03）

组别	例数	DCLK1	sTim-3
预后生存组	46	4.38 ± 1.10	2.12 ± 0.43
预后死亡组	34	6.64 ± 1.34	3.28 ± 0.69
t值	-	8.276	9.240
P值	-	< 0.001	< 0.001

变量	赋值方式
DCLK1	实测值
sTim-3	实测值

因素	单因素分析			多因素分析
因素	HR	95% CI	P值	HR	95%CI	P值
DCLK1	7.058	3.431-14.519	< 0.001	6.954	4.227-11.440	< 0.001
sTim-3	2.578	2.022-3.287	< 0.001	2.347	1.427-3.861	< 0.001

The prognostic value of serum DCLK1 and sTim⁃3 in patients undergoing radical prostatectomy for prostate cancer

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 25

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Xiaomei CHEN,Anqi WANG,Jizhen YANG,Miao YU. Prognosis and immune correlation analysis of m1A/m5C/m6A/m7G regulated genes in gastric cancer [J]. The Journal of Practical Medicine, 2024, 40(9): 1230-1237.
[2]	Shihe XIAO,Gang LI,Zhonghai LIU,Zhen. LIU. Expression of ZEB2 and CCL20 in glioma tissue and their correlation with prognosis [J]. The Journal of Practical Medicine, 2024, 40(9): 1262-1267.
[3]	Futao DANG,Yingmei TANG,Haiyan FU,Huanlong LI,Jiamin XU,Xu. TAN. The efficacy of artificial （nonbiological） extracorporeal liver support system for primary biliary cholangitis and the effect of this therapy on prognosis [J]. The Journal of Practical Medicine, 2024, 40(7): 955-961.
[4]	Xiya MA,Hu JI,Zehua ZHU,Bo PAN,Qiang XIE,Xiaobo. YAO. The predictive value of ¹⁸F⁃FDG PET/CT metabolic heterogeneity parameters combined with clinical features for the prognosis of esophageal squamous cell carcinoma before definitive radiochemotherapy [J]. The Journal of Practical Medicine, 2024, 40(7): 966-971.
[5]	Yuxin CHENG,Liang LIU,Shiyu DONG,Shengchao LI,Meng ZHANG. Research advances in exosomal proteins， mRNA and non⁃coding RNA regulation of Hepatocellular Carcinoma [J]. The Journal of Practical Medicine, 2024, 40(6): 748-755.
[6]	Sibo LONG,Yan CHEN,Xintong ZHANG,Yanjun YIN,Limei YANG,Maike ZHENG,Chaohong WANG,Qing SUN,Jun YAN,Yiheng SHI,Guangli SHI,Yan ZHAO,Guirong. WANG. Serum levels of procalcitonin， interleukin⁃6 and interleukin⁃8 in patients with COVID⁃19 infection at admission and their significance in patient prognosis [J]. The Journal of Practical Medicine, 2024, 40(4): 471-475.
[7]	Zixu SONG,Guangzheng ZHU,Chenxu GUO,Jiaqi WU,Ligong ZHANG,Jun. QIAN. Expression of SLC35A2 and PFDN2 in breast cancer and its relationship with clinical observables and prognosis [J]. The Journal of Practical Medicine, 2024, 40(4): 496-502.
[8]	Yichen WANG,Yanwen YAN,Meihui SONG,Xiangjun XUE,Wenguang ZHOU,Yuquan LI,Ling QI,Guanghua LI,Xiangzong ZENG. Clinical significance of HOXB4 gene expression levels in myelodysplastic syndromes [J]. The Journal of Practical Medicine, 2024, 40(3): 321-325.
[9]	Jia CUI,Mingming ZHANG,Na WANG,Wei GAO,Fei LI. Correlation analysis of glucose and lipid metabolism indicators combined with antiphospholipid antibodies on prognosis of patients with atherosclerosis [J]. The Journal of Practical Medicine, 2024, 40(3): 326-329.
[10]	Shuang CHEN,Na YANG,Yudong HUANG,Xiangfeng KONG,Jintao LI,Yizhong TANG,Kexiong MA,Yangyang ZHANG,Yuandong ZHANG,Chengde REN. Relationship between serum miR-21 and miR-27b levels and prognosis of patients with renal clear cell carcinoma [J]. The Journal of Practical Medicine, 2024, 40(3): 343-348.
[11]	Jian ZHAO,Songjie LIU,Guanchao ZHANG,Yuhou SHEN,Fengchen LI,Bing XU. Expression of CENPF and miR⁃1⁃3p in the serum of patients with advanced gastric cancer and their correlation with prognosis [J]. The Journal of Practical Medicine, 2024, 40(3): 365-370.
[12]	Zhijie XIA,Jun LI,Qian GAO,Zhicheng LI,Zhongfang. XIA. Effect and prognosis of preauricular fistula treated by double⁃incision tunnel combined with preauricular tissue and cartilage resection [J]. The Journal of Practical Medicine, 2024, 40(22): 3179-3183.
[13]	Chengcai DAI,Zhenxing CHENG,Qianqian TU. The prognostic value of serum cystatin C combined with bedside renal ultrasound in patients with sepsis⁃induced acute kidney injury [J]. The Journal of Practical Medicine, 2024, 40(22): 3226-3231.
[14]	Kaiyang WANG,Jing TAO,Tingting WU,Jiahui YONG,Guoqing LI,Xiang XIE,Yining. YANG. Correlation of novel anthropometric indicators with long⁃term prognosis in patients with acute myocardial infarction [J]. The Journal of Practical Medicine, 2024, 40(21): 3040-3046.
[15]	Pixi WEI,Yu DENG,Cailing ZHAO,Liu XU,Min ZHANG. The expression of WDR5 in cervical cancer tissue and its relationship with clinical and pathological characteristics of patients [J]. The Journal of Practical Medicine, 2024, 40(2): 169-173.