HOXB4基因表达水平在骨髓增生异常综合征中的临床意义

doi:10.3969/j.issn.1006-5725.2024.03.007

Abstract

Abstract:

Objective To investigate the expression of HOXB4 gene in patients with myelodysplastic syndromes （MDS） and its clinical significance in disease progression. Methods mRNA expression of HOXB4 gene in bone marrow mononuclear cells was detected by real?time fluorescence quantitative PCR （RT?qPCR）， and the difference in HOXB4 expression was compared between 49 patients with MDS （MDS group） and 35 patients without MDS （group C）. The relationship of mRNA expression of HOXB4 with disease characteristics and clinical prognosis was explored in MDS patients. Results mRNA expression level of HOXB4 gene was higher in MDS group than that in group C （P < 0.05）. The patients were divided into a high? and a low?expression group according to the median expression level of HOXB4. Leukocyte count was lower in the high?expression group in the low?expression group at the time of initial diagnosis. The proportion of patients with subtypes of primitive cellular hyperplasia， poor prognostic staging and leukemic transformation was higher in the high?expression group than in the low?expression group. Conclusions mRNA expression level of HOXB4 gene has certain relation with AML transformation in MDS patients.

Key words: myelodysplastic syndrome, HOXB4, prognosis, leukemia transformation

CLC Number:

R551

Yichen WANG,Yanwen YAN,Meihui SONG,Xiangjun XUE,Wenguang ZHOU,Yuquan LI,Ling QI,Guanghua LI,Xiangzong ZENG. Clinical significance of HOXB4 gene expression levels in myelodysplastic syndromes[J]. The Journal of Practical Medicine, 2024, 40(3): 321-325.

Figures/Tables 4

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References 27

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基因	引物序列
HOXB4	Forward 5'-CTGGATGCGCAAAGTTCACGTG-3'
	Reverse 5'-CGTGTCAGGTAGCGGTTGTAGT-3'
GAPDH	Forward 5'-GTCTCCTCTGACTTCAACAGCG-3'
	Reverse 5'-ACCACCCTGTTGCTGTAGCCAA-3'

参数	HOXB4低表达组（n = 25）	HOXB4高表达组（n = 24）	t/Z值	P值
年龄［M（P ₂₅，P ₇₅），岁］	63.0（52，70）	61.5（44，71）	-0.084	0.993
性别（例）			-0.150	0.879
男	13	13
女	12	11
白细胞计数（× 10⁹ ·L^-1）	4.16（1.81，6.65）	2.24（1.35，3.30）	-1.970	0.049
中性粒细胞数（× 10⁹ ·L^-1）	1.91（0.86，4.70）	0.60（0.39，2.18）	-1.852	0.064
血红蛋白浓度（× 10⁹ ·L^-1）	68.14 ± 24.09	64.71 ± 16.19	0.466	0.644
血小板计数（× 10⁹ ·L^-1）	58.0（31.5，58.0）	85.0（39.8，303.8）	-0.997	0.342

参数	HOXB4低表达组（n = 25）	HOXB4高表达组（n = 24）	χ²值	P值
MDS不伴原始细胞增多（Non-EB）	16（72.70）	6（33.30）	7.528	0.006
MDS伴原始细胞增多（EB）	9（27.30）	18（66.70）

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Clinical significance of HOXB4 gene expression levels in myelodysplastic syndromes

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