实用医学杂志 ›› 2023, Vol. 39 ›› Issue (13): 1600-1605.doi: 10.3969/j.issn.1006⁃5725.2023.13.002

• 基础研究 • 上一篇    下一篇

神经干细胞来源外泌体对脑缺血再灌注损伤大鼠星形胶质细胞TGF⁃β1信号转导与炎症因子的影响

陈珊 朱俊德 赵雪 刘若静 龙婷婷    

  1. 贵州医科大学基础医学院人体解剖学教研室（贵阳 550025） 
  • 出版日期:2023-07-10 发布日期:2023-07-10
  • 通讯作者: 朱俊德 E⁃mail：jdzhu73@126.com
  • 基金资助:
    国家自然科学基金项目（编号：81660243）；贵州省 自然科学基金项目（编号：黔科合基础⁃ZK［2021，2023］一般 415， 323）；贵州医科大学国家自然基金培育项目（编号：20NSP006） 

Effects of exosomes derived from neural stem cells on TGF⁃β1 signal transduction of astrocytes and inflam⁃ matory factors after cerebral ischemia ⁃reperfusion injury in rats 

CHEN Shan，ZHU Junde，ZHAO Xue， LIU Ruojing，LONG Tingting.   

  1. Department of Human Anatomy，Basic Medical College，Guizhou Medical Universi⁃ ty，Guiyang 550025，China 
  • Online:2023-07-10 Published:2023-07-10
  • Contact: ZHU Junde E⁃mail：jdzhu73@126.com

摘要：

目的 探讨神经干细胞来源外泌体（NSC⁃Exos）对脑缺血再灌注损伤大鼠星形胶质细胞TGF⁃β1 信号转导及炎症因子的影响。方法 40 只 SD 大鼠随机分为 Sham 组、MCAO 组、PBS 组和 NSC⁃Exos 组。建 立 MCAO 模型，侧脑室注射 NSC⁃Exos，3 d 后检测大鼠神经功能缺陷评分，取脑进行相对梗死体积测定、组 织病理学、IL⁃6、IL⁃1β、TGF⁃β1 蛋白表达检测，以及 MAP⁃2 和外泌体标记探针 PKH26 共定位检测。结果 NSC⁃Exos 干预降低损伤大鼠神经功能缺陷评分和脑梗死体积（P < 0.05），大鼠海马 CA3 区神经元数量增 加，GFAP 阳性细胞表达率和神经元细胞凋亡指数降低（P < 0.05），蛋白 IL⁃6、IL⁃1β 表达下降，而 TGF⁃β1 表 达增加（P < 0.05）；PKH26 和 MAP⁃2 双阳性细胞在海马 CA3 区表达。结论 NSC⁃Exos 可能通过影响星形 胶质细胞 TGF⁃β1 信号转导，抑制星形胶质细胞增生与炎症因子 IL⁃6、IL⁃1β 的表达，通过增强 TGF⁃β1 的表 达来修复脑损伤。 

关键词: 外泌体, 脑卒中, 星形胶质细胞, TGF?β1, 炎症因子

Abstract:

Objective To investigate the effects of neural stem cell⁃derived exosomes（NSC⁃Exos）on TGF⁃ β1 signal transduction and inflammatory factors in astrocytes during cerebral ischemia ⁃ reperfusion injury in rats. Methods 40 SD rats were randomly divided into Sham group，MCAO group，PBS group and NSC ⁃Exos group. The rat model of MCAO was established. NSC⁃Exos was injected into right lateral ventricle. After 3 days，the neural deficit score was performed，and the brain was taken for relative infarct volume determined，histopathology and expression detection of IL⁃6，IL⁃1β，and TGF⁃β1 protein respectively. The colocalization of MAP⁃2 and exosome⁃ labeled probe PKH26 was detected. Results NSC ⁃Exos intervention could reduce the neurological deficit score and cerebral infarction volume（P < 0.05），increase the number of neurons，reduce the expression rate of GFAP positive cells and neuronal apoptosis index（P < 0.05），and decrease the expression of protein IL⁃6 and IL⁃1β. TGF⁃ β1 expression was increased（P < 0.05）. PKH26 and MAP ⁃2 positive cells were colocalized in CA3 region of hippocampus. Conclusions NSC⁃Exos may inhibit TGF⁃β1 signaling in astrocytes and repair brain injury by reducing astrogliosis and expression of inflammatory factors while enhancing the expression of TGF⁃β1. 

Key words: exosome, stroke, astrocytes, TGF?β1, inflammatory cytokines

粤ICP备18046265号
版权所有 © 2019 《实用医学杂志》编辑部
地址：广州市越秀区惠福西路进步里2号之6　
邮编：510180　电话：020-81866302；81872080；81840509；81922330　
E-mail： syyxzz@syyxzz.com
本系统由北京玛格泰克科技发展有限公司设计开发