关键词: 长链非编码RNA, 雄激素受体, 前列腺癌, 增殖, 侵袭

Abstract:

Objective To study the expression of long non⁃coding RNA（lncRNA）ZNF571⁃AS1 inprostate cancer，to clarify its effect on the proliferation and invasion of prostate cancer cells and its molecularmechanism. Methods Real⁃time quantitative polymerase chain reaction（qPCR）was used to detect the expres⁃sion of ZNF571⁃AS1 in prostate cancer tissues，adjacent tissues，prostate cancer cell lines and normal prostateepithelial cell. The cell line with the most expression of ZNF571⁃AS1 was used as a study. The plasmid thatsilenced the ZNF571⁃AS1 gene and the negative control plasmid were transfected separately. The effects of ZNF571⁃AS1 on the proliferation and invasion of prostate cancer cells were detected by cell proliferation experiment（MTTmethod）and Transwell experiment. Bioinformatics technology predicts the molecular mechanism of ZNF571⁃AS1.qPCR and Western blot methods were used to detect the expression of downstream target genes. Results Theexpression of ZNF571⁃AS1 in prostate cancer tissue was significantly higher than that in adjacent tissues（P < 0.01）.The expression of ZNF571⁃AS1 in prostate cancer cells was significantly higher than that in normal prostate epithelialcells（P < 0.01），and the expression in DU⁃145 cells was the most（P < 0.01）. Low expression of ZNF571⁃AS1can significantly inhibit the proliferation and invasion ability of DU⁃145 cells（P < 0.05）. The downstream targetgene of ZNF571⁃AS1 may be miR⁃301b⁃3p，and the target gene of miR⁃301b⁃3p may be androgen receptor（AR）.Low expression of ZNF571⁃AS1 can significantly up⁃regulate the expression of miR⁃301b⁃3p in DU⁃145 cells（P < 0.01），and inhibit the expression of AR gene（P < 0.01）. Conclusion ZNF571⁃AS1 is significantly up⁃regulated in prostate cancer tissues and cell lines. ZNF571⁃AS1 may affect the proliferation and invasion of prostatecancer DU⁃145 cells by regulating the miR⁃301b⁃3p/AR signaling pathway.

Key words: long?chain non?coding RNA, androgen receptor, prostate cancer, proliferation, invasion