实用医学杂志 ›› 2022, Vol. 38 ›› Issue (9): 1094-1101.doi: 10.3969/j.issn.1006⁃5725.2022.09.010

• 基础研究 • 上一篇    下一篇

右美托咪定对脑出血大鼠神经功能及JAK2/STAT3 通路的影响

郭庆夺1 杨秋影2 马美娜1 于红1 王旭鹏1 吴春玲1 李睿1 何庆1   

  1. 沧州市中心医院1 麻醉科,2 供应室(河北沧州 061000)
  • 出版日期:2022-05-10 发布日期:2022-05-10
  • 基金资助:
    沧州市重点研发计划指导项目(编号:172302105)

Effects of dexmedetomidine on neurological function and JAK2/STAT3 pathway in rats with intracerebral hemorrhage

GUO Qingduo*,YANG Qiuying,MA Meina,YU Hong,WANG Xupeng,WU Chunling,LI Rui,HE Qing.   

  1. Department of AnesthesiologyCangzhou Central HospitalCangzhou 061000China
  • Online:2022-05-10 Published:2022-05-10
  • Supported by:
    基金项目:沧州市重点研发计划指导项目(编号:172302105)

摘要:

目的 研究右美托咪定(Dex)对脑出血大鼠神经功能及蛋白酪氨酸激酶 2(JAK2)/信号转导 和转录激活因子 3(STAT3)通路的影响。方法 成年雄性 SD 大鼠随机分组,进行脑出血造模,给予 Dex NC JAK2 慢病毒干预。评估神经功能缺损评分(NSS),检测血肿周围脑组织病理改变、细胞凋亡及炎症 水平。结果 造模后,脑出血组的 DSS 评分均低于假手术组,细胞凋亡率、NF⁃κB、Bax、Cleaved caspase⁃3 p⁃JAK2、p⁃STAT3 的表达水平、TNF⁃α、IL⁃6 的含量均高于假手术组(P < 0.05);Dex 干预后,Dex 组的 DSS 分均高于脑出血组,细胞凋亡率、NF⁃κB、Bax、Cleaved caspase⁃3、p⁃JAK2、p⁃STAT3 的表达水平、TNF⁃α、IL⁃6 的含量均低于脑出血组(P < 0.05);造模前给予 JAK2 慢病毒侧脑室注射、使 p⁃JAK2 p⁃STAT3 过表达后, JAK2 慢病毒+Dex 组的 DSS 评分均低于 NC 慢病毒+Dex 组,细胞凋亡率、NF⁃κB、Bax、Cleaved caspase⁃3 p⁃JAK2、p⁃STAT3 的表达水平、TNF⁃α、IL⁃6 的含量均高于 NC 慢病毒+Dex 组(P < 0.05)。结论 Dex 改善脑 出血大鼠的神经功能及血肿周围脑组织病理改变,这一改善作用与抑制 JAK2/STAT3 通路介导的炎症反 应及细胞凋亡有关。

关键词:

脑出血, 右美托咪定, JAK2/STAT3通路, 炎症反应, 细胞凋亡

Abstract:

Objective To study the effect of dexmedetomidine(Dex)on neural function and Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3(STAT3)pathway in rats with intracerebral hemorrhage. Methods Adult male SD rats were randomly divided into different groups. The model of intracerebral hemorrhage was established,Dex,NC or JAK2 lentivirus were given for intervention. The neurological deficit score(NSS)was evaluated;the pathological changes,the apoptosis and inflammation levels were evaluated. Results After modeling the DSS scores of intracerebral hemorrhage group were lower than those of the sham group,the apoptosis rate and the expression of NF⁃κB,Bax,Cleaved caspase⁃3,p⁃JAK2,p⁃STAT3,the contents of TNF⁃α,IL⁃6 in brain tissue around hematoma were higher than those of sham group(P < 0.05). After Dex intervention,the DSS scores of Dex group were higher than those of intracerebral hemorrhage group,and the apoptosis rate and the expression of NF⁃κB Bax,Cleaved caspase⁃3,p⁃JAK2,p⁃STAT3,the contents of TNF⁃α,IL⁃6 in brain tissue around hematoma were lower than those of intracerebral hemorrhage group(P < 0.05). After intracerebroventricular injection of JAK2 lentivirus and overexpression of p⁃JAK2,p⁃STAT3 before modeling,the DSS scores of JAK2 lentivirus+Dex group were lower than those of the NC lentivirus + Dex group,the apoptosis rate and the expression of NF ⁃ κB,Bax Cleaved caspase⁃3,p⁃JAK2,p⁃STAT3,the contents of TNF⁃α,IL⁃6 in brain tissue around hematoma were higher than those of the NC lentivirus+Dex group(P < 0.05). Conclusion Dex could improve the neurological function and pathological changes of brain tissue around hematoma in rats with intracerebral hemorrhage. This improvement was associated with the inhibition of JAK2 / STAT3 pathway mediated inflammatory response and apoptosis.

Key words:

intracerebral hemorrhage, dexmedetomidine, JAK2/STAT3 pathway, inflammatory response, apoptosis