长链非编码RNA SPINT1⁃AS1抑制三阴性乳腺癌细胞增殖和促进凋亡的研究

doi:10.3969/j.issn.1006⁃5725.2022.18.002

实用医学杂志 ›› 2022, Vol. 38 ›› Issue (18): 2253-2258.doi: 10.3969/j.issn.1006⁃5725.2022.18.002

长链非编码RNA SPINT1⁃AS1抑制三阴性乳腺癌细胞增殖和促进凋亡的研究

刘春汕杜坤朋王栢耀刘威田允鸿

广州医科大学附属肿瘤医院放疗科（广州510095）

出版日期:2022-09-25 发布日期:2022-09-25
通讯作者: 田允鸿 E⁃mail：tianyunhong2020@gzhmu.edu.cn
基金资助:
国家自然科学基金面上项目（编号：81872195）

Long non ⁃ coding RNA SPINT1⁃ AS1 inhibits proliferation of three negative breast cancer cells and pro⁃ motes their apoptosis

LIU Chunshan，DU Kunpeng，WANG Baiyao，LIU Wei，TIAN Yunhong.

Department of Radiation Oncology，the Affiliated Cancer Hospital，Guangzhou Medical University，Guangzhou 510095，China

Online:2022-09-25 Published:2022-09-25
Contact: TIAN Yunhong E⁃mail：tianyunhong2020@gzhmu.edu.cn

摘要/Abstract

摘要：

目的三阴性乳腺癌（triple negative breast cancer，TNBC）缺少有效治疗靶点，长链非编码 RNA（long non⁃coding RNA，lncRNA）在 TNBC 发生发展中起着重要作用，本实验旨在探究 lncRNA SPINT1⁃ AS1 对 TNBC 细胞增殖与凋亡的影响，寻找治疗 TNBC 的潜在靶标。方法 qRT⁃PCR 检测 SPINT1⁃AS1 在正常乳腺上皮细胞系以及 TNBC 细胞系的表达量；荧光原位杂交法检测 SPINT1⁃AS1 细胞内定位；构建 SPINT1⁃AS1 稳定过表达和低表达的 TNBC 细胞株，克隆形成实验、CCK⁃8 实验、流式细胞术检测 SPINT1⁃ AS1 对 TNBC 细胞增殖和凋亡的影响。结果 SPINT1⁃AS1 主要表达于胞质，在 TNBC 细胞中表达量显著低于正常乳腺上皮细胞，差异有统计学意义（P < 0.05）；SPINT1⁃AS1能显著下调细胞增殖能力（P < 0.05），促进细胞凋亡（P < 0.05）。结论 SPINT1⁃AS1 在 TNBC 细胞中低表达并且主要表达于细胞质；它能抑制 TNBC 细胞增殖并促进其凋亡。这提示SPINT1⁃AS1是TNBC 潜在的治疗靶标。

关键词:

SPINT1?AS1, 三阴性乳腺癌, 细胞增殖, 细胞凋亡

Abstract:

Objective Triple negative breast cancer（TNBC）is an aggressive cancer without effective therapeutic targets. Long non⁃coding RNA（lncRNA）has been shown to play an important role in the development of TNBC. This study is intended to investigate the effects of lncRNA SPINT1⁃AS1 on the proliferation and apoptosis of TNBC cells and find a potential therapeutic target for TNBC. Methods The relative expression of SPINT1⁃AS1 in normal breast epithelial cell lines and TNBC cell lines was detected by real⁃time quantitative PCR（qRT⁃PCR）. The cell localization of SPINT1⁃AS1 was detected by RNA fluorescence in situ hybridization. Overexpressed and down⁃regulated SPINT1⁃AS1 of TNBC cell lines were constructed respectively，and the proliferation and apoptosis of TNBC were explored by clone formation assay，CCK⁃8 assay and flow cytometry. Results SPINT1⁃AS1 was expressed mainly in the cytoplasm. The expression of SPINT1⁃AS1 in the TNBC cells was significantly lower than that in normal breast epithelial cells（P < 0.05）. SPINT1⁃AS1 downregulated the proliferation of TNBC cells in a significant way（P < 0.05），and promoted their apoptosis（P < 0.05）. Conclusion SPINT1⁃AS1 is expressed at a lower level in the cytoplasm of TNBC cells. It can suppress the proliferation of TNBC cells and induce their apopto⁃ sis，which indicates that it can be a potential therapeutic target for treating TNBC.

Key words: , SPINT1?AS1； , triple negative breast cancer； , proliferation； , apoptosis

刘春汕杜坤朋王栢耀刘威田允鸿. 长链非编码RNA SPINT1⁃AS1抑制三阴性乳腺癌细胞增殖和促进凋亡的研究 [J]. 实用医学杂志, 2022, 38(18): 2253-2258.

LIU Chunshan, DU Kunpeng, WANG Baiyao, LIU Wei, TIAN Yunhong..

Long non ⁃ coding RNA SPINT1⁃ AS1 inhibits proliferation of three negative breast cancer cells and pro⁃ motes their apoptosis [J]. The Journal of Practical Medicine, 2022, 38(18): 2253-2258.

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长链非编码RNA SPINT1⁃AS1抑制三阴性乳腺癌细胞增殖和促进凋亡的研究

Long non ⁃ coding RNA SPINT1⁃ AS1 inhibits proliferation of three negative breast cancer cells and pro⁃ motes their apoptosis

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