Abstract:

Objective Minocycline has been shown to be protective on liver injury in uncontrolled hemor⁃ rhagic shock models，but the specific mechanism is not fully understood. Methods After anesthesia and catheter⁃ ization，SD rats were divided into 4 groups：sham operation group（blank group），fluid resuscitation group（con⁃ trol group），MK ⁃ 886 treatment group（experimental group 1），and minocycline treatment group（experimental group 2）. Uncontrolled hemorrhagic shock model was prepared after successful catheterization. ELISA was used to detect TNF⁃α at 3.5h，IL⁃6，LTS，LTB4 and 5⁃LOX；Malondialdehyde（MDA）and myeloperoxidase（MPO）in liver tissue were analyzed and the pathological section was prepared. Automatic biochemical analyzer was used to detect the liver function for 3.5 h：alanine aminotransferase（ALT）and aspartate aminotransferase（AST）. Results The mortality and fluid replacement volume of experimental group 1 and 2 were significantly lower than those of control group，and the mortality and fluid replacement volume of experimental group 2 were lower than those of experimental group 1. The values of TNF⁃α and IL⁃6 in experimental group 2 were significantly lower than those in experimental group 1 and control group（P < 0.05）. The values of liver function（ALT and AST）in experi⁃ mental group 1 and experimental group 2 were significantly lower than those in experimental group 1 and control group（P < 0.05）. The values of LTS，LTB4 and 5⁃LOX in liver tissue of experimental group 1 and experimental group 2 were significantly lower than those of control group（P < 0.05）. Compared with experimental group 2，MDA and MPO of liver and tissue in control group were higher than those in experimental group 1 and 2（P < 0.05；The values of MDA and MPO in liver tissue of experimental group 1 were similar to those of experimental group 2 （P > 0.05），but lower than those of control group. The degree of liver tissue injury in experimental group 1 and experimental group 2 was less than that in control group. There was no significant difference in the Suzuki′ s score of experimental group 1 was compared with that of experimental group 2（P > 0.05）. Conclusions In uncon⁃ trolled hemorrhagic shock models，MC may play an anti⁃inflammatory role by inhibiting 5⁃LOX，and then inhibit inflammatory mediators and cytokines such as TNF⁃α，IL⁃6，LTS，LTB4，MAD and MPO，so as to reduce liver tissue and cell damage after ischemia and reperfusion and play a protective role in liver tissue.

Key words:

minocycline, 5?LOX, hemorrhagic shock, liver