瞬时受体电位通道5对间歇性低氧致心肌焦亡的影响

doi:10.3969/j.issn.1006-5725.2024.12.005

Abstract

Abstract:

Objective To investigate the effect of TRPC5 on intermittent hypoxia-induced myocardial pyroptosis. Methods TRPC5 knock out （TRPC5^-/-） and SD rats were each randomly divided into chronic intermittent hypoxia group （CIH group） and normoxia group （Control group）（n = 6 for each group）. The myocardial fibrosis in rats was visualized by Masson staining， the levels of serum inflammatory factors were measured by ELISA and the relative expression levels of TRPC 5 and pyroptosis-related proteins were determined by Western blotting. Results Masson staining showed that the collagen volume fraction in the TRPC5^-/--CIH group was higher than that in the TRPC5^-/--Control group and lower than that in the WT-CIH group. The ELISA results showed that the serum levels of IL-1， IL-6， and TGF-β in the TRPC5^-/--CIH group were higher than those in the TRPC5^-/--Control group and lower in the WT-CIH group. Western blotting results showed that the relative expression levels of pyroptosis related proteins caspase-1， NLRP3， GSDMD， and GSDMD-N in the TRPC5^-/--CIH group were higher than those in the TRPC5^-/--Control group and lower than those in the WT-CIH group. Conclusion TRPC5 deficiency alleviates myocardial pyroptosis and myocardial fibrosis induced by hypoxia.

Key words: transient receptor potential channel 5, inflammatory factor, pyroptosis, intermittent hypoxia, OSAHS

CLC Number:

R542.2

Xuan QIU,Yishajiang SHAREZATI,Yulan CHEN,Mengmeng WANG,Yu LI,Tulahong GULINAZI,Abuhan ZUBAIDAN,Abulizi ALIYA,Xingchen WANG. Effect of transient receptor potential channel 5 on myocardial pyroptosis in intermittent hypoxia[J]. The Journal of Practical Medicine, 2024, 40(12): 1637-1642.

Figures/Tables 6

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References 28

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组别	胶原容积分数
WT-Control组	2.78 ± 0.32
WT-CIH组	14.23 ± 2.27^a
TRPC5^-/--Control组	3.04 ± 0.44
TRPC5^-/--CIH组	8.30 ± 1.75^b,c
F值	157.915
P值	0.000

组别	IL-1β	IL-6	TNF-α
WT-Control组	49.72 ± 10.79	11.82 ± 2.59	17.44 ± 4.76
WT-CIH组	86.81 ± 17.92^a	27.61 ± 6.15^a	31.23 ± 1.87^a
TRPC5^-/--Control组	40.50 ± 10.43	10.46 ± 2.75	13.31 ± 2.15
TRPC5^-/--CIH组	64.00 ± 11.54^b,c	20.45 ± 4.09^b,c	23.26 ± 2.94^b,c
F值	14.374	23.518	36.810
P值	0.000	0.000	0.000

组别	TRPC5	Caspase?1	NLRP3	GSDMD	GSDMD?N
WT?Control组	0.560 ± 0.059	0.778 ± 0.089	0.551 ± 0.070	0.609 ± 0.037	0.502 ± 0.079
WT?CIH组	0.874 ± 0.072^a	1.108 ± 0.044^a	0.927 ± 0.037^a	0.929 ± 0.053^a	0.758 ± 0.073^a
TRPC5^-/-?Control组	0.114 ± 0.057^a	0.720 ± 0.017	0.515 ± 0.032	0.573 ± 0.033	0.481 ± 0.027
TRPC5^-/-?CIH组	0.321 ± 0.083^b,c	0.907 ± 0.064^b,c	0.773 ± 0.043^b,c	0.727 ± 0.042^b,c	0.623 ± 0.039^b,c
F值	54.938	57.597	57.526	57.054	56.881
P值	0.000	0.000	0.000	0.000	0.000

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Effect of transient receptor potential channel 5 on myocardial pyroptosis in intermittent hypoxia

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