Objective To explore the mechanism of A kinase anchor-protein 150 （AKAP150） in alleviating learning and memory impairment induced by propofol in rats at developmental stage. Methods 80 SD rats aged 7 days were randomly divided into a control group （Con group）， propofol group （Pro group）， dexmedetomidine pre-administration group （DP group）， and AKAP150 adenovirus plus DP group （ADP group） （n = 20）. The Con group was injected with equal volume normal saline. The Pro group was injected with propofol of 50 mg/kg twice， and the DP group was injected with dexmedetomidine of 25 μg/kg plus propofol of 50 mg/kg. The ADP group was treated with adenovirus to construct an AKAP150 knockout model. Expression levels of AKAP150， PKA， NLRP3， GSDMD， IL-1β and IL-18 in hippocampus were detected by Western blot. The ultrastructure of hippocampus was observed by transmission electron microscopy. Results After propofol treatment， hippocampal cell membrane split and pores formed， expressions of AKAP150 and PKA were down-regulated （P < 0.05）， while expressions of NLRP3， GSDMD， IL-1β and IL-18 were up-regulated （P < 0.05）， and the frequency of crossing the platform was decreased （P < 0.05）. After pre-administration of dexmedetomidine， the cell membrane structure of hippocampus was basically normal， expressions of AKAP150 and PKA were up-regulated （P < 0.05）， expressions of NLRP3， GSDMD， IL-1β and IL-18 were down-regulated （P < 0.05）， and the frequency of crossing the platform was increased （P < 0.05）. Conclusions Dexmedetomidine may enhance the activity of PKA and inhibit expressions of NLRP3， GSDMD， IL-1β and IL-18 by activating expression of AKAP150， thereby alleviating propofol-induced pyroptosis in hippocampal tissues and improving learning and memory impairment in rats at developmental stage.

Objective To investigate the protective effect of target temperature management based different durations with delayed hypothermia for traumatic brain injury（TBI） in rats. Methods 36 healthy adult male SD rats were randomly divided into NT group， HT4h group， HT24h group and HT48h group. The TBI model of rats was prepared with an electronic controllable cortical injury device. NT group was treated with normal temperature （37 ℃） 4 h after TBI， and the experimental groups were treated with low temperature （33.0 ± 1.0 ℃） 4 h after TBI for 4 h， 24 h， and 48 h. Three days after TBI， the motor function of the rats in each group was evaluated by beam walking test and inclined-grid climbing test， EB staining was used to measure the blood-brain barrier permeability，the change of hippocampal neurons was observed by Nissl staining， the expression of DCX and GFAP was detected by immunofluorescence， and the expression of Bcl-2 and Bax was measured by Western blot and immunohistochemistry. Results Compared with NT group， the experimental groups could significantly improve the motor function of TBI rats， reduce the permeability of blood-brain barrier，protect hippocampal neurons， promote DCX expression， inhibit GFAP expression， up-regulate the expression of Bcl-2 protein， and down-regulate the expression of Bax protein. However， the protective effect was more apparent in HT48h group than other experimental groups （P < 0.05）. Conclusion Long-term delayed target temperature management has a significant brain protective effect.

Objective Investigating the impact of miR?15a?5p on autophagy in trophoblast cells of preeclamptic placenta. Methods Collect 20 cases of normal placental tissue and 20 cases of preeclamptic placental tissue from December 2020 to December 2022. Use fluorescence quantitative PCR to detect the expression of miR?15a?5p in placental tissue and trophoblast cells， and study its correlation with patient blood pressure. The HTR8?S/Vneo cells are divided into normal group （control） and hypoxia group， and the effect of hypoxia on the expression of miR?15a?5p is observed. Additionally， mimic?NC group， mimic?NC+hypoxia group， miR?15a?5p mimic group， miR?15a?5p mimic+hypoxia group， inhibitor?NC group， inhibitor?NC+hypoxia group， miR ?15a ?5p inhibitor group， and miR ?15a ?5p inhibitor +hypoxia groups are set up to observe the effect of miR ?15a ?5p on hypoxia?induced autophagy?related proteins LC3B and p62 protein in trophoblast cells. Western blot is used to detect the expression levels of autophagy?related proteins LC3B and p62 protein in each group； TargetScan website predicts the target genes of miR?15a?5p，and detects their expression levels in placental tissue and trophoblast cells. Results Compared with the control group， the expression levels of miR?15a?5p were significantly increased in the placentas and hypoxic trophoblasts of preeclampsia， and they were positively correlated with the blood pressure of the patients. Under hypoxic conditions， the overexpressed miR?15a?5p promoted the protein expression of LC3BII/I， while the relative expression of P62 was decreased. But after interference with miR?15a?5p， LC3BII/I expression was down?regulated and P62 expression was up?regulated. The results of quantitative PCR and Western blot showed that the expression levels of YAP1 in the preeclampsia placental tissues and hypoxic trophoblasts were significantly reduced. Conclusion The upregulation of miR?15a?5p in trophoblast cells of the placenta in individuals with preeclampsia could enhance autophagy in preeclampsia by forming a complex with YAP1.

Objective To investigate the effect of TRPC5 on intermittent hypoxia-induced myocardial pyroptosis. Methods TRPC5 knock out （TRPC5^-/-） and SD rats were each randomly divided into chronic intermittent hypoxia group （CIH group） and normoxia group （Control group） （n = 6 for each group）. The myocardial fibrosis in rats was visualized by Masson staining， the levels of serum inflammatory factors were measured by ELISA and the relative expression levels of TRPC 5 and pyroptosis-related proteins were determined by Western blotting. Results Masson staining showed that the collagen volume fraction in the TRPC5^-/--CIH group was higher than that in the TRPC5^-/--Control group and lower than that in the WT-CIH group. The ELISA results showed that the serum levels of IL-1， IL-6， and TGF-β in the TRPC5^-/--CIH group were higher than those in the TRPC5^-/--Control group and lower in the WT-CIH group. Western blotting results showed that the relative expression levels of pyroptosis related proteins caspase-1， NLRP3， GSDMD， and GSDMD-N in the TRPC5^-/--CIH group were higher than those in the TRPC5^-/--Control group and lower than those in the WT-CIH group. Conclusion TRPC5 deficiency alleviates myocardial pyroptosis and myocardial fibrosis induced by hypoxia.

Objective To examine the regulation of malignant progression of colorectal cancer by LINC00626 via the JAK1/STAT3/KHSRP signaling axis and its molecular mechanism. Methods 96 individuals diagnosed with colorectal cancer at our hospital during June 11， 2021 and June 11， 2023 were chosen as research subjects， and their cancerous tissue and nearby normal tissue were collected. Cultivate colorectal cancer cell lines （SW620， HCT116， HT29， DLD-1， LOVO， Caco-2） and normal colorectal cells （NCM460） in vitro， and detect the expression of LINC00626 and KHSRP in colorectal cancer tissue and cell lines using qRT-PCR. Screening out cell lines infected with lentivirus， SW620 and HCT116 cell lines were transfected with knockdown lentivirus and its control， while HT29 and DLD-1 cell lines were transfected with overexpressing lentivirus and its control， respectively. Select stable transfected cell lines for cell function experiments to detect proliferation， migration， and invasion abilities. Detection of the effect of LINC00626 on the growth and migration of colorectal cancer tumors in live mouse experiments. The expression level of KHSRP protein in stable labeled cells was determined using a western blot analysis. Rescue experimental research on the regulatory relationship between LINC00626 and KHSRP. Results qRT-PCR showed low expression of LINC00626 and high expression of KHSRP in colorectal cancer tissues and cell lines. Cell function experiments showed that compared with the sh-NC group， the sh-LINC00626 group promoted cell proliferation， migration， and invasion in SW620 and HCT116 cells， while the overexpression group showed the opposite. Cell rescue experiments showed that， LINC00626+KHSRP significantly reversed the promotion effects of knocking down LINC00626 on cell proliferation， migration， and invasion. In the nude mouse experiment， compared with the sh-NC group， the sh-LINC00626 group showed a significant increase in tumor volume and weight， cell proliferation rate， and the number of lung metastases from colorectal cancer in the nude mice； Overexpression results in the opposite. The signal pathway experiment revealed that relative to the sh-NC group， the expression levels of JAK1 and STAT3 mRNA in the sh-LINC00626 group were significantly increased， whereas the results in the overexpression group were the opposite. Conclusion LINC00626 suppression the malignant progression of colorectal cancer metastasis through the JAK1/STAT3/KHSRP signaling axis.

Objective To investigate the role of preoperative 3D printing simulation technology in the interventional endovascular exclusion of Stanford B aortic dissection. Methods A retrospective study protocol was used to select 76 patients with Stanford B-type aortic dissection who underwent endovascular isolation surgery in our hospital from January 2019 to January 2021 in the study. Among them， 40 patients underwent simulated surgery with preoperative 3D printing simulation technology （simulation group） and another 36 patients received conventional endovascular isolation surgery （control group）. The two groups were compared in terms of the.cardiac ultrasound indicators， the true and false lumen diameters， and the complication rates of the proximal aortic rupture plane， stent end plane， and abdominal trunk artery plane at different times before and after surgery. Results As compared to the control group， the simulation group showed significantly longer surgical time， extracorporeal circulation time， aortic occlusion time， and ICU stay time （all P < 0.05）. After the operation， the true lumen diameter of the proximal aortic rupture plane， stent end plane， and abdominal trunk artery plane were significantly increased in two groups at months 1， 3， and 6 （P < 0.05）， but the false lumen diameter of the proximal aortic rupture plane， stent end plane， and abdominal trunk artery plane were significantly decreased （P < 0.05） compared to preoperative levels. The LVEF and FS of the two groups were significantly increased 6 months after surgery （P < 0.05）. Conclusion The preoperative 3D printing simulation technology in the interventional endovascular isolation of Stanford B aortic dissection can ensure the surgical effect. It can achieve the same surgical effect as the traditional procedures and importantly it can reduce the difficulty of operation and shorten the operation time.

Objective To establish an optimal limiting dose for dose limiting rings placed in the anterior and posterior regions of the neck for reducing head and neck lymphedema under intensity-modulated radiation therapy（IMRT） for early nasopharyngeal carcinoma（NPC） from a dosimetric perspective. Method Fifteen newly diagnosed early-stage nasopharyngeal carcinoma patients who underwent CT localization for radiotherapy at the Cancer Hospital of Guangzhou Medical University from January to September 2022 were included in the study. Each case was designed with five sets of radiotherapy plans. Plan A consisted of conventional unlimited-field plans， while Plans B-E consisted of limited-field plans with dose constraints set at 20， 18， 16， and 14 Gy， respectively， with the remaining parameters consistent with Plan A. The impact on target coverage and organ-at-risk constraints was evaluated through variance analysis and pairwise multiple comparisons using a randomized block design to determine the optimal dose limits. Results The gradient of 16Gy was determined as the optimal dose limiting cutoff point for achieving the balance between target coverage and organ limiting dose. Compared with the conventional plan， The plans with the placement of a cervical anterior and posterior dose limiting ring （16Gy） did not change the target dose coverage （P > 0.05）， but only yielded a slight change in the homogeneity index （P < 0.05）. It did not cause any changes of the dosage in the inner ear， mandible， and brainstem （all P > 0.05）， but lead to statistically significant reductions in the oral cavity， throat， and thyroid （all P < 0.05）. It caused a slight increase of the dose in the parotid gland and spinal cord （both P < 0.05）， but the increased dose was anyhow within the tolerance range. Conclusion The dosimetric investigation determines an optimal dose limit cutoff point for the cervical anterior and posterior dose limiting rings. It is expected to provide a design method for IMRT plans to reduce head and neck lymphedema after radiotherapy for early NPC.

Objective To explore the efficacy of repetitive peripheral magnetic stimulation （rPMS） in patients with lumbar disc herniation （LDH）. Methods From March 2023 to March 2024，60 LDH patients were recruited in the inpatient or outpatient department of the rehabilitation department of a tertiary hospital. All patients were randomly assigned to the rPMS group or the conventional group， 30 cases in each group. Both groups received routine physical therapy， and the rPMS group was treated with rPMS on this basis.VAS， JOA， and neurophysiological tests were performed before intervention and 2 weeks after intervention. Results The VAS and JOA scores of the two groups were significantly lower than those before treatment （P < 0.05）. Compared with the conventional group， the VAS and JOA scores of the rPMS group were significantly lower （P < 0.05）. Compared before and after treatment， the neuroelectrophysiological examination of the rPMS group was significantly improved （P < 0.05）. After 2 weeks of treatment， the tibial nerve motor conduction velocity， H reflex latency and IP peak in the conventional group were significantly faster than those before treatment（P < 0.05）. After 2 weeks of treatment， compared with the conventional group， there were significant differences in tibial nerve motor conduction velocity， peroneal nerve motor conduction velocity， superficial peroneal nerve sensory conduction velocity， sural nerve sensory conduction velocity， H reflex latency and IP peak （P < 0.05）. Conclusion rPMS can significantly improve and restore pain and nerve injury in patients with LDH. rPMS can be used as an effective adjuvant therapy.

Objective To investigate changes in serum complement C1 tumor necrosis factor-related protein family 12 （CTRP12） level before and after percutaneous coronary intervention （PCI） in patients with acute myocardial infarction （AMI） and the relationship of CTRP12 level with in-stent restenosis （ISR）. Methods A total of 104 patients who had been diagnosed with AMI and had undergone PCI at Danyang People's Hospital in Jiangsu Province from January 2021 to June 2023 were selected. The incidence of ISR within 12 months after PCI was counted， and they were divided into an ISR group and a non-ISR group according to the results of reviewed coronary angiography. Serum CTRP12 levels were compared between the two groups before PCI and on one day before discharge. Logistic regression was used to analyze the influencing factors of ISR in AMI patients after PCI. Receiver operating characteristic （ROC） curve was used to analyze the predictive value of CTRP12 for ISR in AMI patients after PCI. Results The incidence of ISR in 104 AMI patients at 12 months after PCI was 14.4% （15/104）. As compared with the non-ISR group， the ISR group had significant increases in preoperative TIMI flow of ≤ 1， white blood cell count， neutrophil count， TC， and LDL-C， and a significant decline in serum CTRP12 level on one day before discharge （P < 0.05）. In the non-ISR group， serum CTRP12 level was significantly higher on one day before discharge than its baseline （P < 0.05）. In the ISR group， serum CTRP12 level on one day before discharge was lower than its baseline， but the difference was not statistically significant （P > 0.05）. Logistic regression analysis showed that a lower CTRP12 level on one day before discharge was an independent risk factor for ISR in AMI patients after PCI （P < 0.05）. ROC curve analysis showed that the optimal cut-off point of serum CTRP12 on one day before discharge for predicting ISR in AMI patients after PCI was 3.89 ng/mL （sensitivity 93.3% and specificity 73.0%）， and the area under the ROC curve （AUC） was 0.849. Conclusions Serum CTRP12 level inone day before discharge has certain predictive value for ISR in AMI patients after PCI. CTRP12 may be a therapeutic target for ISR in AMI patients after PCI.

Objective To evaluate the clinical effect of Tension-free laparoscopic lateral suspension with mesh for pelvic organ prolapse. Methods A total of 85 patients who underwent pelvic organ prolapse were selected as the study group， and 40 patients underwent Laparoscopic sacral fixation surgery（LSC） as the control group in Henan Provincial People's Hospital from March 1， 2021 to October 31， 2023. The patients were divided into two subgroups： uterine preservation group and uterine resection group and followed up until April 30， 2024. The intraoperative conditions and postoperative complications were recorded and analyzed. POP quantitative staging（POP-Q）scores were used for evaluation. Preoperative and postoperative quality of life and therapeutic effect were evaluated using the pelvic floor distress inventory short form-20（PFDI-20）， urinary distress inventory-6 （UDI-6）， colorectal-anal distress inventory-8 （CRADI-8）， pelvic organ prolapse distress inventory-6（POPDI-6）， pelvic floor impact questionnaire-7（PFIQ-7）， prolapse and Incontinence sexual function questionnaire short form（PISQ-12）. Results The median follow-up time for patients in the study group is 13.13 months， with an objective cure rate of 96.47% and a reoperation rate of 1.18%. The perioperative complication rates are 6.45% for uterine resection and 4.35% for uterine preservation， while the mesh exposure rate is 1.61% for uterine resection. In comparison， the median follow-up time for patients in the control group is slightly longer at 13.76 months， with an objective cure rate of 92.5% and a reoperation rate of 2.5%. The perioperative complication rates are higher at 14.71% for uterine resection and as high as 33.33% for uterine preservation， while the mesh exposure rate is also elevated at 8.82% for uterine resection. Despite these differences， there was no significant disparity in objective cure rates or reoperation rates between the study group and the control group. Furthermore， it was observed that the study group experienced shorter operation times， less bleeding， faster postoperative recovery， shorter hospitalization periods， lower perioperative complications， and reduced mesh exposure rates?especially among patients with uterine preservation. Additionally， intra-group comparisons revealed significant improvements in all POP-Q indicators one year after surgery （P < 0.05）， along with significantly lower scores on PFDI-20， UDI-6， CRADI-8， POPDI-6， PFIQ-7， and PISQ-12 scales compared to pre-surgery levels （P < 0.05）. However， no significant inter-group differences were noted. Conclusions Tension-free laparoscopic lateral suspension with mesh proves to be an effective surgical approach for treating anteriorand middle pelvic organ prolapse. It demonstrates few perioperative complications while significantly improving prolapse symptoms and enhancing patient qualityof life. It stands as a viable alternative to sacrocolpopexy， particularly beneficial for patients with a preserved uterus.

Objective To investigate the effect of percutaneous endoscopic transforaminal discectomy （PETD） on L₅-S₁ lumbar disc herniation （LDH） and the influence of iliac crest height on the clinical efficacy. Methods A total of 86 patients treated with PETD for LDH （L₅-S₁ segment） from February 2019 to February 2018 were selected and grouped according to the relationship between the highest point of the iliac crest and the position of the L_4-5 pedicle. Forty-eighty patients with the highest point of the iliac crest located below the upper edge of the L₅ pedicle were included in group A； thirty-three patients with the highest point of the iliac crest located between the lower edge of the L₄ pedicle and the upper edge of the L₅ pedicle were included in group B， and five patients with the highest point of the iliac crest located above the lower edge of the L₄ pedicle were included in group C. The operation indexes of the three groups were compared. The visual analogue score （VAS） and Oswestry Disability index （ODI） before and after surgery ［preoperative（T₀）， 1 week after surgery （T₁）， 1 month， 6 months and 12 months after surgery （T₂，T₃，T₄）］were compared among the three groups. Results There was no difference in operation time and blood loss among the three groups （P > 0.05）. At T₀， there was no difference in VAS score and ODI among the three groups （P > 0.05）. At T₁-T₄， when VAS score and ODI of the three groups were lower than that at T₀， VAS in group A and B was lower than that in group C （P < 0.05）， but there was no difference between group A and B （P > 0.05）. Conclusion PETD has significantly clinical efficacy in the treatment of L₅-S₁ LDH， and whether the iliac crest height is higher than the level of the lower edge of the L₄ pedicle will affect its clinical efficacy.

Objective To explore the efficacy and prognosis of robot-assisted percutaneous pedicle screw （PPS） in the treatment of thoracic and lumbar spine fracture. Methods A total of 84 patients with thoracolumbar fracture were selected from Xuzhou Renci Hospital from November 2018 to November 2022， and divided into study group （42 cases with robot-assisted PPS） and control group （42 cases with free-hand PPS） according to random number rank method. Perioperative indexes， nail placement accuracy， prognostic indexes （VAS score， Cobb Angle， relative height of anterior vertebra） and incidence of postoperative complications were compared between the two groups. Results Less intraoperative blood loss， shorter fluoroscopy time， fluoroscopy times， operative time， single nail placement time and radiation exposure time， and higher nail placement accuracy were observed in the study group （P < 0.05）. VAS score and Cobb Angle of the injured vertebrae were lower in the postoperative 3 d and the last follow-up， and the relative height of the injured vertebrae was higher than that before surgery in the two groups （P < 0.05）. Conclusion Robot-assisted PPS in the treatment of thoracolumbar fracture has a good application effect， which can shorten the operation time， reduce the intraoperative fluoroscopy times， improve the accuracy of nail placement， and have good safety.

Objective To explore the early prediction value of related test indicators for non-biliary acute pancreatitis and the influence of interaction of related test indicators on non-biliary acute pancreatitis. Methods A total of 280 patients with the first onset of non-biliary acute pancreatitis who were hospitalized in Jinshan Hospital of Fudan University from July 2019 to July 2023 were divided into mild group and severe group. The severe group included moderately severe and severe patients. Clinical data， tests and examination indicators of first-attack inpatients within 48 hours after admission were collected. Through logistics regression analysis， early detection indicators related to the severity of non-biliary acute pancreatitis were explored， and the influence of their interaction was studied. Results Single-factor analysis showed that albumin （ALB）， thyroxine （T4）， serum free triiodothyronine， albumin （ALB）， Thyroxine （T4） in both groups. FT3）， thyroid stimulating hormone （TSH）， serum triglyceride （TG）， calcium （Ca） and other indicators were statistically significant （P < 0.05）； Through multi-factor logistics regression analysis， it was found that the OR values of T4， FT3， TSH， CA and other detection indicators were all less than 1 （P < 0.05）； After controlling for gender， age， history of hypertension， history of diabetes and other factors， the interaction between TG and HbA1c was analyzed， the aOR was 1.500 when only HbA1c was increased （P > 0.05）， the aOR was 4.488 when only TG was increased （P < 0.05）， and the aOR was 5.084 when TG and HbA1c were increased simultaneously （P < 0.05）. Conclusion ALB， T4， FT3， TSH， CA were protective factors of non-biliary AP， and could be used as predictive indicators of the severity of non-biliary acute pancreatitis. Interaction of HbA1c with TG increases the risk of progression to severe non-biliary acute pancreatitis.

Objective To investigate the efficacy of indobuprofen combined with nicodil for the treatment of acute coronary syndromes （ACS） and the effects of percutaneous coronary intervention on patients Effects of myocardial injury markers， platelet function and inflammatory factors after intervention （PCI）. Method A total of 150 patients with ACS admitted to the hospital from January 2021 to December 2022 were divided into groups according to different treatment methods. The control group （n = 75） was given nicodil combined with antiplatelet therapy， and the study group （n = 75） was given indobufen combined with nicodil combined with antiplatelet therapy. Both groups were treated for 2 weeks. The clinical efficacy of the two groups was compared， the changes of myocardial injury markers， platelet function and inflammatory factors before and after treatment were monitored， and the total incidence of adverse reactions was recorded. Results The total effective rate of the group treated with nicodil combined with indobufen was 98.67% higher than that of the control group treated with nicodil alone， 90.67% （χ² = 4.754， P < 0.05）. The levels of myocardial injury markers such as cTnI and CK-MB in the study group after treatment were lower than those in the control group （t = 15.492， 3.250， P < 0.05）. The levels of platelet function indexes such as CD62p， CD63， GPⅡb/Ⅲa in the study group after treatment were lower than those in the control group （t = 2.034， 3.257， 2.221， P < 0.05）. The levels of CRP， TNF-α， IL-6 and other inflammatory factors in the study group were lower than those in the control group after treatment （t = 21.862， 3.378， 2.131， P < 0.05）. The total incidence of adverse reactions after treatment was 4.00% in the study group and 2.67% in the control group （P > 0.05）. Conclusion The efficacy of indobufien combined with nicodil in the treatment of ACS is better than that of nicodil alone， and it can improve myocardial injury and platelet function after PCI， inhibit the release of inflammatory factors， and the incidence of adverse reactions is lower.

Objective To evaluate the value of adenosine injection in paclitaxel eluting PTCA balloon catheter. Methods A total of 200 patients undergoing drug-coated balloon angioplasty were randomly divided into control group receiving conventional treatment after the procedure， and observation group receiving adenosine injection for 3 hours continuously. All patients underwent coronary SYNTAX II scoring， and CK-MB， NT-proBNP， hs-CRP levels were recorded before and after PTCA for 6 hours， 12 hours， 18 hours， and 24 hours. A dedicated Israeli cardiac machine D-SPECT was used for rest + stress myocardial perfusion radionuclide imaging before and after the PTCA for 7 days. The total myocardial perfusion score under rest + stress myocardial perfusion in 17 segments was observed， as well as the adverse drug reactions after the PTCA. Results There were no significant differences in clinical characteristics， concomitant medication upon admission， coronary SYNTAX II score and PTCA treatment， mean target vessel stenosis， mean balloon diameter and length， CK-MB， NT-proBNP， hs-CRP before PTCA， and total myocardial perfusion score values under resting and stress myocardial perfusion distribution in 17 segments between the two groups（P > 0.05）. The CK-MB， NT-proBNP， and hs-CRP values in the experimental group were significantly lower than those in the control group at 6， 12， 18， and 24 hours after PTCA （P < 0.05）；The total myocardial perfusion score under resting and stress myocardial perfusion distribution in 17 segments was significantly lower in the experimental group than in the control group at 7 days after PTCA（P < 0.05）；There was no significant difference in adverse drug reactions between the two groups of patients after PTCA（P > 0.05）. Conclusions The application of adenosine injection in patients after drug-coated balloon therapy significantly reduced the CK-MB， NT-proBNP， and hs-CRP values 24 hours after PTCA， as well as the total myocardial perfusion score under resting and stress myocardial perfusion distribution in 17 segments 7 days after PTCA， without increasing significant adverse reactions.

Objective To investigate the overall and subgroup efficacy， subgroup differences， predictors of efficacy and safety of roxadustat in the treatment of refractory non-severe aplastic anemia （NSAA） and low-risk myelodysplastic syndromes （LR-MDS）. Methods Patients with refractory NSAA and LR-MDS who were admitted to the Department of Hematology， Peking Union Medical College Hospital from August 2020 to December 2022 were enrolled. All patients received first-line standard treatment， including recombinant human erythropoietin （rhEPO） for at least 3 months before roxadustat treatment. All patients received roxadustat 2.5 mg/kg every other day for at least 3 months， and were followed up for at least 8 months. The clinical characteristics of patients， roxadustat efficacy， predictors of efficacy， adverse effects，relapse and disease clonal evolution were analyzed. Results A total of 40 patients including 24 refractory NSAA and 16 LR-MDS were included. median age was 56 （18 ~ 81） years and 40% were males. 65% of the patients were transfusion dependent. Median follow-up was 21 （9 ~ 34） months. 22.5%， 25.0%， 47.5% ， 55.0%， 57.5%， 60.0% and 50.0% of the patients achieved haematological improvement-erythroid （HI-E） at months 1， 2， 3， 4， 5， 6， and end of the follow-up period， respectively， and no factors affecting HI-E were identified.The hemoglobin change from baseline was statistically different between the two groups at the end of the follow-up period. 50% of patients were relieved from transfusion dependence. Adverse reactions were reported in 22.5% of patients. 28.5% of patients relapsed after achieving HI-E， with a median time to relapse of 7（4 ~ 12） months. No clonal evolution was observed at the end of the follow-up period. Conclusions Our preliminary findings suggested that Roxadustat may be effective for patients with NSAA or LR-MDS refractory to conventional therapies and rhEPO， with mild adverse effects and low relapse rate.The degree of hemoglobin improvement may be better in the refractory NSAA patients.

Objective To explore the correlation between the expression level of interferon gamma （IFN-γ） and the severity of patients with septic shock and its prognostic value. Methods The clinical data and serum of 96 septic shock patients admitted to the Department of Critical Care Medicine， the First Affiliated Hospital of Guangxi Medical University from March 2022 to August 2023 were collected， and divided into survival group and death group according to the 28-day outcome； collected at the same time Sera from 30 healthy people undergoing physical examination during the same period served as the control group. The enzyme-linked immunosorbent （ELISA） method was used to detect the expression levels of IFN-γ in the three groups， and the expression levels of IFN-γ and various clinical data between the groups were analyzed. Results The serum IFN-γ expression level of patients with septic shock was lower than that of healthy people undergoing physical examination， 212.80 （151.11， 255.79） ng/L compared with 343.37 （314.5， 427.95） ng/L， P < 0.01. Among the 96 cases of septic shock， There were 54 cases in the survival group and 42 cases in the death group. The clinical data of the two groups were compared. The gender， smoking history， SBP， DBP， SI， Lac， IL-6， IFN-γ， PCT， SOFA score， and APACHEⅡscore of the two groups of patients were compared. The difference is statistically significant （P < 0.01）. Logistic regression analysis showed that decreased expression of Lac， IFN-γ， and APACHEⅡ score were independent risk factors for death in patients with septic shock. ［Odds ratio （OR） and 95% confidence interval （95%CI） were 6.491 （1.404 ~ 30.004） respectively. 0.954 （0.954 ~ 0.999）， 3.476 （1.210 ~ 9.984）， P < 0.05］. The Spearman correlation analysis of INF-γ， Lac and APACHEⅡ showed a negative correlation， and the correlation coefficients were -0.38 and -0.35 respectively. Drawing the ROC curves of Lac， IFN-γ， and APACHEII， the AUCs of the three were 0.847， 0.869， and 0.833 respectively. The AUC of the three joint predictions was 0.978. The joint prediction value of the three was better than that of a single indicator， P < 0.001. Conclusion The decrease in IFN-γ expression level and the severity of septic shock patients have good prognostic value.

Objective To investigate the association between triglyceride glucose index（TyG） combined with waist circumference （WC） and the risk of new-onset coronary heart disease （CHD） in the middle-aged and elderly people in 10 years of non-interventional follow-up in Luzhou area. Methods A total of 8 647 eligible individuals participated in epidemiological survey in Luzhou area were selected. The subjects were divided into different groups according to the quartiles of the TyG-WC and followed for ten years. The correlation between different TyG-WC quartile group and 10-year new CHD in middle-aged and elderly population was analyzed by using univariate analysis and Cox regression analysis. Results Of the 8 647 participants in the study， 484 had new CHD， accounting for 5.5%. The comparison of the number of newly diagnosed CHD subjects grouped by TyG-WC quartiles indicated the 4^th quartile group > the 3^rd quartile group > the 2^nd quartile group > the 1^st quartile group， showing statistical significance （P < 0.001）. Cox regression analysis showed that without adjusting for any variables， the risk of new CHD in 10 years increased with the increase of TyG-WC quartile group， and the differences between the second， third and fourth quartile array and the first quartile array were statistically significant （P < 0.05）. After adjusting for age and sex and further adjusting for the influence of various influencing factors， the risk of new CHD in 10 years increased with the increase of TyG-WC quartile group. The difference between the third and fourth quartile array and the first quartile array was statistically significant （P < 0.05）. Conclusion TyG-WC is a risk factor for new-onset CHD in the middle-aged and elderly population in Luzhou area in 10 years of non-interventional follow-up， and TyG-WC may be a predictor of the risk of CHD in the middle-aged and elderly population.

Objective To establish a method for detecting the 2019 novel coronavirus subgenomic RNA （sgRNA）， and evaluate the performance of the established method. Methods Primers and probes were designed according to the subgenomic sequence of the 2019 novel coronavirus， and a reverse transcription PCR method for sgRNA detection was established. The established method was optimized， including the concentration and proportion of primers and probes， extension temperature， reaction volume and template amount. The performance of the method was evaluated， including the limit of detection， sensitivity， specificity and repeatability. sgRNA and genomic RNA （gRNA） were detected in clinical samples， and the results were analyzed. Results In this study， an RT-PCR method for the detection of sgRNA of the 2019 novel coronavirus was established. The limit of detection of this method was 100 copies/mL， and the detection results of common pathogens were negative. The CV of sgRNA in high， medium and low concentration samples were less than 5%. sgRNA was positive in 115 suspected 2019 novel coronavirus infected patients （115/330， 34.85%）. When the Ct value of gRNA-N was less than 30， the positive rate of sgRNA was 100.00%. When the Ct value of gRNA-N was in the range of 30-32， the positive rate of sgRNA was 68.75%. When the Ct value of gRNA-N was in the range of 32-35， the positive rate of sgRNA was 44.44%. When the Ct value of gRNA-N was greater than 35， the sgRNA was negative. Conclusion The RT-PCR method for the detection of sgRNA of the 2019 novel coronavirus was established， and the detection method was sensitive， specific and reproducible.

Objective To explore the functional improvement effect of acupuncture and moxibustion therapy of traditional Chinese medicine （mainly acupuncture on the three yang meridians of the hand， moxibustion on the governor vessel， and moxibustion through drugs） on cervical spondylosis of wind cold blocking collateral type. Methods 120 patients with wind cold obstruction type cervical spondylosis admitted to our hospital from July 2021 to July 2023 were randomly divided into two groups， with 60 cases in each group， using a random number table method. The control group was treated with conventional cervical traction， while the observation group was treated with traditional Chinese medicine acupuncture and moxibustion on the basis of the control group （mainly acupuncture at the hand Sanyang meridian+moxibustion at the governor's meridian+moxibustion through drugs）. Compare the TCM syndrome scores， NDI， HO-1， LpPLA2， clinical efficacy， adverse reactions， hospitalization costs， and duration between two groups. Results After 4 weeks of treatment， the effective rate of disease in the observation group with wind cold obstructing cervical spondylosis （96.67%） was higher than that in the control group （83.33%）， while the traditional Chinese medicine syndrome score， NDI score， HO-1， LpPLA2 in the observation group were lower than those in the control group （P < 0.05）. No adverse reactions were observed in both groups. Conclusion Traditional Chinese medicine acupuncture and moxibustion therapy （mainly acupuncture of the three yang meridians of the hand， governor vessel moxibustion， and herbal penetration moxibustion） has significant clinical effect in the application of cervical spondylosis of wind cold blocking collateral type， which can effectively improve clinical symptoms and biochemical indicators， promote the rehabilitation of cervical function， and has high safety.

Cellular senescence and fibrosis are two biological processes that are closely related to the development of many diseases. Cellular senescence can occur through mechanisms such as telomere shortening， DNA damage， and oxidative stress， leading to degradation of cell function and decreased ability to repair damage. More and more studies have shown that fibrosis and cell senescence are closely related， and cell senescence has been confirmed to be involved in the occurrence and development of scar fibrosis diseases. An in-depth understanding of the relationship between cellular senescence and scar fibrosis is helpful to find new therapeutic strategies and develop targeted drugs to reduce the process of scar fibrosis.

Stroke is one of the main diseases endangering the health and life of the middle-aged and elderly， and in recent years it shows a younger trend.It has the characteristics of high incidence， mortality and disability rate. Atherosclerosis is the main intervention target for stroke prevention and treatment.CircRNA is highly expressed in the cerebrovascular system and plays an important regulatory role in the pathogenesis of ischemia-reperfusion and ischemic stroke.This paper reviews the mechanism of circRNA and miRNA molecular network in the pathophysiological process of atherosclerosis， ischemic stroke and ischemia-reperfusion injury， so as to provide theoretical support for the application of circRNA in gene diagnosis and drug treatment of atherosclerotic ischemic stroke.

T-cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain （TIGIT） is a transmembrane glycoprotein expressed on the surface of immune cells. TIGIT expression is up-regulated in bladder cancer and will have an important impact on bladder cancer development and poor prognosis. Immunotherapy that blocks TIGIT signaling is expected to improve the prognosis of bladder cancer. With further research， TIGIT is likely to become a target for bladder cancer diagnosis and treatment. This article reviews the structure， immunomodulatory effect and the research progress of TIGIT in bladder cancer.