Abstract:

Objective To investigate the effect of long intergenic non⁃coding RNA 00689（LINC00689） on the proliferation and apoptosis of bladder cancer cell T24 and its possible mechanism. Methods Real ⁃time fluorescence quantitative PCR（RT⁃qPCR）was used to analyze the expression of LINC00689 and miR⁃876⁃5p in bladder cancer tissues，adjacent tissues，T24 cells and normal bladder epithelial cells SV⁃HUC⁃1. Dual luciferase reporter gene and RT ⁃qPCR were applied to verify the regulation effect of LINC00689 on miR ⁃876⁃5p. T24 cells were divided into si⁃NC group，si⁃LINC00689 group，miR⁃NC group，miR⁃876⁃5p group，si⁃LINC00689 + anti⁃miR⁃ NC group and si⁃LINC00689 + anti⁃miR⁃876⁃5p group. Cell counting kit（CCK⁃8）and flow cytometry were used to analyze cell viability and apoptosis and western blot was used to analyze expression of cyclin D1（CyclinD1），p21， B cell lymphoma 2（Bcl⁃2）and Bcl⁃related X protein（Bax）. Results The expression of LINC00689 was increased in bladder cancer tissues and T24 cells，but that of miR⁃876⁃5p was decreased（P < 0.05）. LINC00689 negatively regulated miR⁃876⁃5p expression. Compared with those in si⁃NC group，cell viability，expression of CyclinD1 but Bcl⁃2 proteins were reduced，and apoptosis rate，expression of p21 and Bax proteins were increased in si⁃LINC00689 group（P < 0.05）. Compared with those in miR⁃NC group，cell viability，expression of CyclinD1 and Bcl⁃2 pro⁃ teins were reduced，but apoptosis rate，expression of p21 and Bax proteins were increased in miR⁃876⁃5p group （P < 0.05）. Compared with those in si⁃LINC00689 + anti⁃miR⁃NC group，cell viability，expression of CyclinD1 and Bcl ⁃ 2 proteins were increased，but apoptosis rate，expression of p21 and Bax proteins were reduced in si ⁃ LINC00689 + anti⁃miR⁃876⁃5p group（P < 0.05）. Conclusion Inhibiting LINC00689 can reduce the proliferation 

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