白细胞介素-17基因多态性与山西东南地区汉族人群2型糖尿病的相关性

doi:10.3969/j.issn.1006-5725.2024.05.018

Abstract

Abstract:

Objective To determine the correlation between single-nucleotide polymorphisms of IL-17 with type 2 diabetes in Han population in southeastern Shanxi Provincein. Methods The basic information and related clinical data of the subjects were collected of normal healthy controls and T2DM. Whole blood DNA was extracted， and IL-17 polymorphisms （rs2275913， rs3819024， rs4711998 and rs8193036） were analyzed by using a polymerase chain reaction-high temperature ligase reaction. Results There was no significant difference in the genotype and allele frequency distribution of the four SNP loci of IL-17 gene between the two groups （P > 0.05）. IL-17 polymorphism was not linked with T2DM in multiple genetic models after controlling for age， BMI， WC， and dyslipidemia （P > 0.05）. Further analysis showed that the levels of AA genotype was substantially lower of FPG and HOMA-IR levels when compared with the AG and GG genotypes， and the differences among the three groups were statistically significant （P < 0.05）. However， BMI， FINS， HbA1c and HOMA-βwas no statistical significance among the three groups （P > 0.05）. Besides， WC and HOMA-IR of AA genotype （rs3819024） were notable higher than those of GG genotype in T2DM group（P < 0.05）. Conclusions The IL-17 gene polymorphisms rs2275913， rs3819024， rs4711998， and rs8193036 in the Han population of southeast Shanxi Province may not be associated with T2DM. In this region Han T2DM patients， AA genotype at rs2275913 of the IL-17 gene is associated to FPG and HOMA-IR， while GG genotype at rs3819024 is related to WC and HOMA-IR， which could be the potential genotype of IL-17 impacting glucose metabolism.

Key words: type 2 diabetes mellitus, interleukin-17, genetic polymorphism

CLC Number:

R394

Yan LI,Qi WANG,Lu ZHENG,Lihui FENG,Li MA,Jian WEI,Liangshu. LIU. Correlation between IL-17 gene polymorphism and type 2 diabetes mellitus in Han population in southeast Shanxi Province[J]. The Journal of Practical Medicine, 2024, 40(5): 695-701.

Figures/Tables 5

Tab.1

Tab.2

Tab.3

Tab.4

Tab.5

Comparison of clinical index levels among each genotype of IL-17 gene SNP locus in T2DM population"

相关指标	rs2275913基因型			F值	P值
相关指标	AA	GA	GG	F值	P值
BMI（kg/m²）	25.95 ± 3.68	25.55 ± 3.01	26.51 ± 3.70	0.594	0.325
WC（cm）	90.03 ± 1.86	92.27 ± 9.38	93.89 ± 8.38	0.945	0.416
FPG（mmol/L）	8.02 ± 0.66	10.27 ± 0.46	10.63 ± 0.58^a	3.791	0.026
HbA_1c（%）	8.84 ± 0.63	9.12 ± 0.24	9.32 ± 0.33	0.335	0.716
FINS（mIU/L）	10.10 ± 1.07	11.75 ± 0.68	12.22 ± 0.76	1.133	0.326
HOMA?IR	3.51 ± 0.44	5.13 ± 0.31	5.57 ± 0.40^a	4.928	0.009
HOMA?β	64.99 ± 11.66	47.66 ± 5.58	58.84 ± 13.81	0.591	0.556
相关指标	rs3819024基因型			F值	P值
相关指标	AA	GA	GG	F值	P值
BMI（kg/m²）	26.67 ± 3.47	25.84 ± 3.23	25.17 ± 3.27	1.377	0.188
WC（cm）	95.26 ± 8.10	92.04 ± 9.01	88.41 ± 10.90^a	3.401	0.032
FPG（mmol/L）	11.07 ± 0.52	9.68 ± 0.46	9.10 ± 0.90	2.694	0.073
HbA_1c（%）	9.54 ± 0.33	8.83 ± 0.24	9.28 ± 0.56	1.438	0.242
FINS（mIU/L）	11.35 ± 0.73	12.36 ± 0.69	10.20 ± 1.10	1.530	0.222
HOMA?IR	5.49 ± 0.39	5.14 ± 0.31	3.83 ± 0.50^a	3.407	0.037
HOMA?β	38.84 ± 3.73	58.77 ± 9.53	68.86 ± 16.83	2.407	0.095
相关指标	rs4711998基因型			F值	P值
相关指标	AA	AG	GG	F值	P值
BMI（kg/m²）	26.12 ± 3.27	25.76 ± 3.27	26.33 ± 3.27	0.197	0.648
WC（cm）	92.75 ± 3.17	91.61 ± 3.21	93.88 ± 3.33	0.350	0.786
FPG（mmol/L）	9.78 ± 0.55	10.04 ± 0.47	10.83 ± 0.79	0.541	0.584
HbA_1c（%）	9.37 ± 0.31	8.68 ± 0.23	9.72 ± 0.61	2.209	0.115
FIns（mIU/L）	11.46 ± 0.62	11.39 ± 0.79	12.98 ± 1.36	0.663	0.517
HOMA?IR	4.74 ± 0.30	4.95 ± 0.36	6.11 ± 0.72	2.045	0.134
HOMA?β	55.73 ± 7.38	53.59 ± 11.50	42.85 ± 6.65	0.261	0.771
相关指标	rs8193036基因型			F值	P值
相关指标	CC	TC	TT	F值	P值
BMI（kg/m²）	25.80 ± 3.14	26.14 ± 3.66	26.16 ± 2.78	0.127	0.781
WC（cm）	91.00 ± 8.88	93.37 ± 9.78	93.82 ± 9.02	0.870	0.244
FPG（mmol/L）	10.44 ± 0.58	9.37 ± 0.40	11.04 ± 0.95	1.855	0.257
HbA_1c（%）	9.41 ± 0.31	8.8 ± 0.25	9.46 ± 0.64	1.349	0.407
FIns（mIU/L）	10.96 ± 0.83	12.0 ± 0.54	12.58 ± 1.49	0.839	0.336
HOMA?IR	4.81 ± 0.38	4.92 ± 0.28	6.02 ± 0.77	1.508	0.315
HOMA?β	50.97 ± 8.42	58.49 ± 10.0	40.76 ± 6.80	0.511	0.190

Tab.5

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观察指标	CON	T2DM	χ²/t值	P值
例数（男/女）	103（60/43）	101（61/40）	0.097	0.755
年龄（岁）	40.25 ± 8.50	42.34 ± 7.78	1.831	0.069
BMI（kg/m²）	23.21 ± 3.05	25.71 ± 3.35	5.272	< 0.001
WC（cm）	81.68 ± 8.97	92.44 ± 9.31	8.411	< 0.001
HC（cm）	97.40 ± 6.52	97.74 ± 10.74	0.270	0.788
WHR	0.84 ± 0.05	0.99 ± 0.51	2.960	0.003
FPG（mmol/L）	4.79 ± 0.36	10.03 ± 3.32	15.945	< 0.001
2hPG（mmol/L）	5.53 ± 0.59	18.59 ± 4.47	29.346	< 0.001
HbA_1c（%）	5.66 ± 0.65	9.14 ± 1.91	17.483	< 0.001
FIns（mIU/L）	5.92 ± 3.22	11.64 ± 4.67	10.200	< 0.001
HOMA?IR	1.26 ± 0.07	5.02 ± 0.23	16.058	< 0.001
HOMA?β	100.01 ± 6.46	53.07 ± 5.80	5.399	< 0.001
TG（mmol/L）	1.15 ± 0.36	2.11 ± 1.80	5.291	< 0.001
TC（mmol/L）	3.87 ± 0.66	4.82 ± 1.37	6.350	< 0.001
HDL?C（mmol/L）	1.33 ± 0.25	1.03 ± 0.22	9.146	< 0.001
LDL?C（mmol/L）	2.57 ± 0.53	3.12 ± 0.96	5.067	< 0.001

多态性位点	基因型频率			等位基因频率频次
多态性位点	CC	TC	TT	C	T
rs2275913	66（32.4）	100（49.0）	38（18.6）	232（56.9）	176（43.1）
rs3819024	66（32.3）	103（54.5）	35（17.2）	235（57.6）	173（42.4）
rs4711998	91（44.6）	92（45.1）	21（10.3）	274（67.2）	134（32.8）
rs8193036	84（41.1）	95（46.6）	25（12.3）	263（64.5）	145（35.5）

SNP位点	等位基因/基因型	频率［n（%）］		χ²值	P值
SNP位点	等位基因/基因型	CON	T2DM	χ²值	P值
rs2275913	A	92（44.7）	84（41.6）	0.393	0.531
	G	114（55.3）	118（58.4）
	AA	22（21.4）	16（15.8）	1.088	0.580
	AG	48（24.6）	52（51.5）
	GG	33（32.0）	33（32.7）
rs3819024	A	116（57.4）	119（57.8）	0.034	0.853
	G	87（42.2）	86（42.6）
	AA	33（32.7）	33（32.0）	0.096	0.953
	GA	53（51.5）	50（49.5）
	GG	17（16.5）	18（17.8）
rs4711998	A	141（68.4）	133（65.8）	0.314	0.575
	G	65（31.6）	69（34.2）
	AA	45（43.7）	46（45.5）	3.412	0.182
	AG	51（49.5）	41（40.6）
	GG	7（6.8）	14（13.9）
rs8193036	C	130（64.4）	133（64.6）	0.310	0.965
	T	73（35.4）	72（35.6）
	CC	42（41.6）	42（40.8）	0.115	0.944
	TC	49（47.6）	46（45.5）
	TT	12（11.7）	13（12.9）

分组	rs2275913
	共显性模型			显性模型		隐性模型
	AA	AG	GG	GG	AA，AG	GG，AG	AA
CON	22（21.4）	48（24.6）	33（32.0）	33（32.0）	70（68.0）	81（78.6）	22（21.4）
T2DM	16（15.8）	52（51.5）	33（32.7）	33（32.7）	68（67.3）	85（84.2）	16（15.8）
OR（95%CI）	0.72（0.26~2.00）	0.60（0.19~190）	1	1	1.02（0.89~1.17）	1	1.03（0.94~1.14）
P值	0.300	0.768	_	_	0.923	_	0.496
P′值	0.289	0.526	_	_	0.731	_	0.313

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Correlation between IL-17 gene polymorphism and type 2 diabetes mellitus in Han population in southeast Shanxi Province

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