Abstract:

Objective To investigate the clinical value of peripheral blood microRNA（miRNA）⁃92a and miR ⁃342 in the diagnosis and prognosis of patients with acute lung injury（ALI）. Method Sixty patients with ALI were divided into ALI survival group（n = 40）and ALI death group（n = 20）according to their survival status during follow⁃up，and 52 healthy subjects were selected as control group. The expression levels of miR⁃92a and miR⁃ 342 in the peripheral blood were analyzed by RT⁃PCR，and the clinical data of the patients were collected. Cox regression was used to analyze the risk factors of death in the ALI patients，and receiver operating characteristic （ROC）curves were plotted to evaluate the diagnostic value of miR ⁃ 92a and miR ⁃ 342 in ALI patients. Results Compared with the control group，the APACHEII score and LIPS score were higher and CRP in the peripheral blood was higher in the ALI survival group and ALI death group. The results of qRT⁃PCR analysis showed that the relative expression level of miR ⁃92a in the ALI survival group was higher than in the control group（P < 0.05）， and higher in the ALI death group than in the ALI survival group（P < 0.05. The relative expression level of miR⁃ 342 in the ALI survival group was lower than in the control group（P < 0.05），and the relative expression level of miR⁃342 in the ALI death group was significantly lower than in the ALI survival group（P < 0.05）. The results of Cox regression analysis showed that peripheral blood CRP，miR ⁃92a and miR ⁃342 levels were independent risk factors affecting the risk of ALI（P < 0.05）. ROC results showed that miR⁃92a had an area under the curve of 0.865， a sensitivity of 84.39%，a specificity of 73.29%，and a cutoff value of 2.16；miR⁃342 had an area under the curve of 0.839，a sensitivity of 82.19%，a specificity of 71.77%，and a cutoff value of 0.56. The MiR⁃92a and miR⁃342 combined diagnostic area under the curve was 0.895，with a sensitivity of 86.87%，and a specificity of 76.57%.Conclusion The levels of miR⁃92a and miR⁃342 in peripheral blood are related to acute lung injury，and the com⁃ bined detection of miR⁃92a and miR⁃342 can provide clinical evidence for the early prediction of acute lung injury. 

Key words:

acute lung injury, microRNA, diagnosis and prognosis, clinical value