Abstract:

Objective To analyze the effect of METTL3 mediated IGF2BP3 m6A modification on the proliferation and migration of hepatoblastoma cells in children，and provide basis for clinical treatment. Methods the expression of METTL3 and IGF2BP3 mRNA in children hepatoblastoma HuH ⁃6 was detected by QRT PCR ； Western blot was used to detect the expression of METTL3 and IGF2BP3. By lentiviral transfection，METTL3 was knocked down in HuH⁃6 cells，and the expression of METTL3 protein was verified by immunoblotting. The expres⁃ sion of IGF2BP3 was detected by QRT PCR and Western blot，and the m6A methylation RNA immunoprecipitation technique was used to analyze the m6A modification of IGF2BP3 mRNA；The cell proliferation ability was detected by CCK⁃8 assay，and the cell migration ability was detected by Transwell cell assay. Results METTL3 sgRNA intervention significantly decreased the expression level of METTL3 in cells，and the difference was statistically significant（P < 0.05）；The cell proliferation level of sgRNA group was significantly lower than that of empty vector group at 48 h and 72 h，and the difference was statistically significant（P < 0.05）；The cell migration of sgRNA group was significantly lower than that of empty vector group at 24 h and 48 h，and the cell invasion level of sgRNA group was significantly lower than that of empty vector group；The expression levels of IGF2BP3 protein and mRNA in sgRNA group were significantly lower than that in empty vector group（P < 0.05）；The relative expression of m6A IP and IGF2BP3 m6A in sgRNA group was significantly lower than that in empty vector group （P < 0.05）. Conclusion METTL3 can effectively regulate the modification of IGF2BP3 and m6A and regulate the proliferation and migration of hepatoblastoma cells in children.

Key words:

METTL3, IGF2BP3 m6A modification, hepatoblastoma, cell proliferation, cell migration, children