Endometriosis（EM）is a common gynecological disease with an increasing incidence yearly. It mainly causes chronic pelvic pain and infertility. Due to the lack of“root cause”therapy for the etiology，the recur⁃ rence rate after conventional surgery is very high except radical surgery. Premenopausal patients need long ⁃term medication to prevent its recurrence. Moreover，there is no sensitive index for its early non⁃invasive diagnosis and monitoring of its early changes. Existing studies suggest that EM is closely related with microbial dysregulation in vivo. In this paper，we exploredthe possible ways by which microorganisms participate in the occurrence of EM. We concluded by reviewing literature that intestinal microorganisms may participate in the pathogenesis of EM byintervening lipid metabolism，estrogen metabolism and other ways，microorganisms and related metabolites may be used as early noninvasive diagnostic markers for EM，and finally，low fat and low carbon diet，consumingunsaturated fatty acids and probiotics supplementation may be a new approach to the prevention and control of EM.

Objective To observe the regulatory effect of emodin（ED）on the expressions of procoagulant and fibrinolysis inhibitory factors in alveolar epithelial cells type Ⅱ（AECⅡ）under LPS stimulation. Methods Cells were given different doses of ED（0.25，0.5，1 μg/mL）treated for 1h and then stimulated with LPS for 24 h. The expressions of TF，TFPI，PAI⁃1 in the cells and the levels of PⅢP，TAT，AT Ⅲ and APC in the cell superna⁃ tant were detected. Results The expressions of TF and PAI⁃1 in LPS stimulated cells significantly increased，while the expression of TFPI decreased. At the same time ，the levels of PⅢP and TAT in the supernatant of cells increased，while the levels of AT Ⅲ and APC decreased，which all were reversed by ED in a dose ⁃dependent manner. Immunofluorescence suggested that LPS promoted the expression of p65 in nucleus，which was significantly inhibited by ED. Conclusion ED，in the dose⁃dependent method，inhibits the expression and secretion of proco⁃ agulant and fibrinolytic inhibition factors in AECⅡ cells stimulated by LPS，which may be related to its inhibition of NF⁃κB signal pathway.

Objective To explore the effect of miR⁃92a⁃3p on glomerular mesangial cell injury induced by high glucose by targeting Homeobox protein 13（HOXA13）and its mechanism. Methods High glucose was used to induce mouse glomerular mesangial cells SV40⁃MES⁃13 to establish a cell injury model. Experimental groups included control group，high glucose group，anti⁃miR⁃NC+ high glucose group，anti⁃miR⁃92a⁃3p+ high glucose group，pcDNA+ high glucose group，pcDNA⁃HOXA13+ high glucose group，anti⁃miR⁃92a⁃3p+ si⁃NC+high glu⁃ cose group，and anti⁃miR⁃92a⁃3p+si⁃HOXA13+high glucose group. QRT⁃PCR and western blot were used to detect the expression of miR⁃92a⁃3p and HOXA13，respectively. Flow cytometry was used to detect the rate of apoptosis； the dual luciferase reporter experiment to detect the targeting relationship between miR⁃92a⁃3p and HOXA13；and western blot to detect the protein expression of Cleaved⁃caspase⁃3，and Cleaved⁃caspase⁃12. Results Compared with those in the control group，the expression of miR⁃92a⁃3p in the high glucose group was increased（P < 0.05）； HOXA13 mRNA and protein levels were decreased（P < 0.05），and the apoptosis rate and the protein levels of Cleaved⁃caspase⁃3，Cleaved⁃caspase⁃12 were increased（P < 0.05）. After transfection of anti⁃miR⁃92a⁃3p or pcDNA⁃ HOXA13，the apoptosis rate and the protein levels of Cleaved⁃caspase⁃3 and Cleaved⁃caspase⁃12 were decreased （P < 0.05）. HOXA13 was a target gene of miR⁃92a⁃3p. Co⁃transfection of anti⁃miR⁃92a⁃3p and si⁃HOXA13 could attenuate the inhibitory effects of anti ⁃miR ⁃ 92a ⁃3p on high glucose induced glomerular mesangial cell apoptosis （P < 0.05）. Conclusion Knocking down miR ⁃ 92a ⁃ 3p can inhibit apoptosis by targeting the expression of HOXA13，thereby reducing the glomerular mesangial cell damage induced by high glucose.

Objective To investigate the effect of sodium butyrate on bone mineral content and bone microstructure in mice with fluorosis. Methods According to the principle of weight balance，24 male ICR weaned mice were divided into control group，fluorosis group and sodium butyrate group. The mice in the fluorosis group and sodium butyrate group drank distilled water containing 100 mg/L sodium fluoride，and those in the control group drank water freely. At week 14，the mice in the sodium butyrate group were given 500 mg/kg sodium butyrate by gavage，while those in the control group and fluorosis group were given normal saline. After 20 weeks， the left tibia and femur of the mice in all the groups were removed，which were subjected to bone structure strength measurements，Micro ⁃CT scanning and bone mineral content measurement. Results Compared with the control group，the trabecular bone mineral density，bone volume fraction，bone trabecular number，bone calcium，bone magnesium，bone zinc content and bone structure strength of the mice in the fluorosis group were all significantly decreased（t = 4.921，P < 0.01；t = 6.959，P < 0.01；t = 4.915，P < 0.05；t = 2.363，P < 0.05；t = 6.553，P < 0.01； t = 2.461，P < 0.05；t = 4.166，P < 0.01，respectively），while the trabecular separation，trabecular structure model index，bone fluoride and bone phosphorus content were all significantly increased（t = -3.490，P < 0.05； t = -3.820，P < 0.05；t = -8.608，P < 0.01；t = -8.641，P < 0.01，respectively）. The bone CT results showed that the trabecular structure was sparse and irregular，and the bone micro⁃structures were damaged obviously. The bone trabecular，bone mineral density，bone calcium，bone magnesium，bone zinc content and bone structure strength of the mice in the sodium butyrate group were all significantly higher than those in the fluorosis group （t = -3.557，P < 0.05；t = -2.444，P < 0.05；t = -3.271，P < 0.01；t = -4.810，P < 0.01；t = -2.552，P < 0.05， respectively），while the bone fluoride and bone phosphorus content was also significantly reduced（t = 4.042，P < 0.01；t = 2.424，P < 0.05，respectively）. Our results showed that the structure of trabecular bone was relativelytight，and the degree of structural damage was relatively mild. Conclusion Sodium butyrate can alleviate the disorder of bone metabolism in mice with fluorosis，improve the microstructure of trabecular bone，and enhance the bone strength.

Objective To analyze the effect of METTL3 mediated IGF2BP3 m6A modification on the proliferation and migration of hepatoblastoma cells in children，and provide basis for clinical treatment. Methods the expression of METTL3 and IGF2BP3 mRNA in children hepatoblastoma HuH ⁃6 was detected by QRT PCR ； Western blot was used to detect the expression of METTL3 and IGF2BP3. By lentiviral transfection，METTL3 was knocked down in HuH⁃6 cells，and the expression of METTL3 protein was verified by immunoblotting. The expres⁃ sion of IGF2BP3 was detected by QRT PCR and Western blot，and the m6A methylation RNA immunoprecipitation technique was used to analyze the m6A modification of IGF2BP3 mRNA；The cell proliferation ability was detected by CCK⁃8 assay，and the cell migration ability was detected by Transwell cell assay. Results METTL3 sgRNA intervention significantly decreased the expression level of METTL3 in cells，and the difference was statistically significant（P < 0.05）；The cell proliferation level of sgRNA group was significantly lower than that of empty vector group at 48 h and 72 h，and the difference was statistically significant（P < 0.05）；The cell migration of sgRNA group was significantly lower than that of empty vector group at 24 h and 48 h，and the cell invasion level of sgRNA group was significantly lower than that of empty vector group；The expression levels of IGF2BP3 protein and mRNA in sgRNA group were significantly lower than that in empty vector group（P < 0.05）；The relative expression of m6A IP and IGF2BP3 m6A in sgRNA group was significantly lower than that in empty vector group （P < 0.05）. Conclusion METTL3 can effectively regulate the modification of IGF2BP3 and m6A and regulate the proliferation and migration of hepatoblastoma cells in children.

Objective To investigate the relationship between genetic variation of super⁃enhancer region and overall survival（OS）of postoperative patients with hepatitis B virus（HBV）related hepatocellular carcinoma （HCC）. Methods A total of 866 patients HCC who underwent hepatectomy in Guangxi Medical University Cancer Hospital from July 2007 to December 2017 were studied. The relationship between genetic variation of super enhancer region and OS were analysed using Kaplan⁃Meier analysis and Cox proportional hazards regression model. Results The rs61805159（G > A）and rs4791170（G > A）A allele were associated with higher risk of death in patients with HCC（P < 0.001），whereas rs546220（C > A）A allele was associated with a lower risk of death（P < 0.001）. Moreover，patients with multiple risk genotypes had a lower survival rate and a higher risk of death mortality risk than those with 0 risk genotypes，and there was a dose⁃response relationship（Ptrend < 0.001）. Conclusion Single or multiple genetic variation of loci in the super⁃enhancer region affected OS of patients with HCC.

Objective To investigate the effect of temporal straight incision small bone window keyhole craniotomy in the treatment of basal ganglia hemorrhage. Methods A retrospective analysis was performed on 72 patients with basal ganglia hemorrhage who underwent craniotomy in the Department of Neurosurgery of the First Affiliated Hospital of Anhui Medical University from August 2018 to February 2022.There were 33 patients who had keyhole craniotomy（observation group）and 39 patients who had large⁃scale craniotomy（control group）. The surgical outcomes of the two groups were compared，including operation time，hematoma clearance ratio，postopera⁃ tive complications，and hospitalization，duration，hospitalization costs，state of consciousness at discharge，and prognosis. Results The comparison between the observation group and the control group showed that the craniotomy time in the two groups was（17.03 ± 4.60）min and（38.18 ± 8.49）min，respectively，the operation time was （130.73 ± 44.70）min and（211.10 ± 60.14）min，respectively. The clearance rates were（92.88 ± 5.33）% and （87.00 ± 15.00）%，respectively. The length of hospital stay was（16.88 ± 8.02）d and（22.79 ± 11.23）d，respec⁃ tively. The incidence of epidural hematoma was 15% and 41%，respectively. The incidence of muscle edema was 12% and 41%，respectively. The hospitalization expenses of the observation group were（10.47 ± 4.35）ten thousandyuan，and the control group was（13.08 ± 5.30）ten thousand yuan. The difference of the above indicators was statistically significant（P < 0.05）. In the observation group and the control group，the ICU stay time was（7.91± 5.40）d and（9.38 ± 8.34）d，the mechanical ventilation time was（6.06 ± 4.80）d and（6.90 ± 5.64）d，the GCS score at discharge was（12.38 ± 2.62）and（12.03 ± 2.40），the proportion of intracranial infection 0% and 5%， and the incidence rate of pulmonary infection was 55% and 51%，the incidence rate of postoperative rebleeding was 3% and 5%，and the incidence rate of postoperative deep vein thrombosis was 12% and 8%. There was no significant difference（P>0.05）. In the ADH assessment of the observation group and the control group at 3 months after operation，the proportion of normal function was（6.2%）and（2.7%），the proportion of mild function impair⁃ ment was（25.0%）and（24.3%），the proportion of moderate function impairment was（34.4%）and（32.4%）， and the proportion of severe function impairment was（28.1%）and（32.4%），death rate（6.3%）and（8.2%）. There was no significant difference in prognosis between the two groups（P > 0.05）. Conclusion In the treatment of basal ganglia hemorrhage，a temporal straight incision small bone window keyhole craniotomy has less trauma，a shorter craniotomy time，a high rate of hematoma removal，a significantly shorter operation time and hospitalization time，fewer complications，and lower hospitalization costs. It has a high safety and clinical application value，as well as good clinical efficacy.

Objective The study aimed to investigate the value of reflectance confocal microscopy（RCM） in evaluating microscopic changes in moderate to severe plaque psoriasis treated with secukizumab. Methods Eleven patients with moderate to severe plaque psoriasis，three to six lesions were selected from each patient and the local psoriasis severity index（LPSI）score was used to evaluate the clinical efficacy before and after 12 weeks of treat⁃ ment；the same lesions were examined using RCM to measure changes in the area，circumference and diameter of the dermal papillae（DP）and capillary loops（CL）clearly identified at the epidermis⁃dermis junction. The groups were divided into upper limb，trunk and lower limb groups according to the site of the lesion，and the differences in DP and CL diameter change rates were compared between the groups；the correlation between DP and CL diame⁃ ter change rates and clinical efficacy was analysed using Spearman′s correlation analysis. Results A total of 53 lesions selected from 11 patients，the area，circumference and diameter of DP and CL were reduced after treatment （P<0.001）. However，12 of the 26 clinically cured（LPSI=0）lesions still had enlarged DP diameters（≥80 μm） and active cells were still visible in the lumen of some CL canals. The rate of change in DP diameter was significantly smaller in lower limb lesions than in upper limb and trunk lesions（P<0.05），with no significant difference in CL diameter change between different sites（P>0.05）. There was a strong correlation between the rate of change in LPSI and the rate of change in DP diameter before and after treatment（rs=0.644 8，P<0.000 1）and a weaker corre⁃ lation with the rate of change in CL diameter（rs=0.339 2，P=0.003 1）. Conclusion Clinical cure of lesions is not the best time to discontinue the biologics，the RCM parameters of DP and CL of psoriatic lesions can be used asmicroscopic indicators to monitor the efficacy of biologics dynamically and provide a more objective basis for biolog⁃ ics reduction and discontinuation.

Objective To study the predictive value of the combination of serum troponin I（cTnI），endo⁃ thelin⁃1（ET⁃1）and hypoxia inducible factor⁃1 α（HIF⁃1 α）for heart failure（HF）. Methods One hundred and seventeen patients with pulmonary hypertension admitted to No.7 People′s Hospital of Zhengzhou from March 2018 to February 2020 were selected and divided into pulmonary hypertension heart failure group and pulmonary hyper⁃ tension group according to whether HF with preserved ejection fraction was found during the 12⁃month follow⁃up. Serum cTnI，ET⁃1 and HIF⁃1 of the two groups were detected and compared. Multivariate Cox proportional hazard regression analysis was used to analyze the influencing factors of HF，and ROC curve was used to evaluate the predictive value of serum cTnI，ET⁃1 and HIF⁃1 αfor HF. Results The difference in PH severity between the two groups was statistically significant（P < 0.05）. Serum cTnI，ET⁃1，HIF⁃1 in PH heart failure group α The levels were higher than those in PH group（all P < 0.05）. Serum cTnI，ET⁃1，HIF⁃1α Elevated level and severity of PH were risk factors for heart failure（all P < 0.05）. And serum cTnI，ET⁃1，HIF⁃1α Compared with the prediction results of each single index，the specificity and AUC of the combination of the three indexes in predicting heart failure were significantly superior（all P < 0.05）. Serum cTnI，ET⁃1，HIF⁃1αThere was no significant difference between the sensitivity of combined prediction of heart failure and that of each index alone（P > 0.05）. Conclusion Increased expression of serum cTnI，ET⁃1，and HIF⁃1α would increase the risk of HF. Serum cTnI，ET⁃1，and HIF⁃1α have predictive value for the occurrence of pulmonary hypertension and HF with preserved emission blood fraction， and the predictive value is higher when the three factors are combined.

Objective This study aimed to preliminarily explore the application of three⁃dimensional visual virtual surgery system combined with self⁃made intervertebral foramen positioning puncture device in percutaneous endoscopic transforaminal decompression（PETD）of lumbar spinal stenosis（LSS）and to evaluate the accuracy， safety and surgical efficacy of this method. Methods A retrospective controlled study was conducted. From January 2021 to December 2021，64 patients（27 male and 37 female，aged（57.1 ± 6.45）years，（range 50 ~ 77 years old）with LSS were treated by PETD. A three⁃dimensional visual virtual surgery system combined with self⁃made intervertebral foramen positioning puncture device was used to establish cannula in 32 patients（visualization group， V group），and traditional conventional methods were used to establish cannula in another 32 patients（routine group，R group）. The hospitalization days，puncture times，puncture time，fluoroscopy times，operation time， visual analog score（VAS），Oswestry dysfunction index（ODI），and complications in the two groups were analyzed. Results The operation and follow ⁃ up were successfully completed in all patients without serious complications. There was no significant difference between two groups in terms of general information，hospitalization days and operation time（P > 0.05）. The V group was better than the R group in puncture time，puncture times and fluoros⁃ copy times，and the differences were statistically significant（P < 0.05）. The VAS score of the two groups after operation was better than that before operation，and the difference was statistically significant（P < 0.05）. The ODI of the two groups after operation was better than that before operation，and the difference was statistically signifi⁃cant（P < 0.05）. Conclusions Through comparative research，this study showed that the three⁃dimensional visual virtual surgery system combined with self⁃made intervertebral foramen positioning puncture device could significantly reduce the puncture times，fluoroscopy times，and puncture time in the treatment of lumbar spinal stenosis using percutaneous endoscopic transforaminal decompression，with high puncture accuracy，safety，and satisfactory surgical effects. Therefore，the proposed method was feasible.

Objective To evaluate the efficacy and safety of PD⁃1 Inhibitor combined with brain radiotherapy for brain metastases in patients with pan negative non⁃small cell lung cancer（NSCLC）. Methods The clinical data of 62 pan ⁃negative NSCLC patients with brain metastases treated at our hospital between August 2016 and August 2021 were retrospectively analyzed to compare the local control rate of brain metastases，incidence of new intracranial lesions，overall survival，progression ⁃ free survival and occurrence of adverse reactions between the group with radiation therapy alone and the group with radiation plus anti⁃PD⁃1 therapy. Results The incidence of new intracranial lesions in the former andlatter groupwas 54.1%（20/37）and 28.0%（7/25），respectively（P = 0.042）. The median progression free survival（PFS）for the latter group was 1.5 months longer than the latter group （P = 0.005）. The median overall survival（OS）of the group with the radiation plus anti ⁃ PD ⁃ 1 therapy was 3.6 months longer than the group with radiation therapy alone（P = 0.016）. Treatment modality was an independent risk factor for both PFS（P = 0.004）and OS（P = 0.01）. The adverse effects could be tolerated. Conclusion For pan⁃negative NSCLC patients with brain metastases，radiation plus anti⁃PD⁃1 therapy may prolong PFS and OS， with the adverse reactions tolerable.

Objective To explore the relationship between insulin resistance（IR），triglyceride ⁃glucose （Tyg）correlation index and cerebral microbleed（CMB）in patients with cerebral small vessel disease（CSVD），and to establish a predictive model. Methods A total of 130 patients with cerebral small vessel disease who were admitted to the Second Affiliated Hospital of Zhengzhou University from January 2021 to May 2022 were selected as clinical research subjects，and they were divided into CMB group（42 cases）and Non⁃CMB group（88 cases）. The basic clinical data of the patients were recorded and the IR ⁃ related indicators［triglyceride ⁃ glucose index （Tyg），triglyceride⁃glucose⁃BMI index（Tyg⁃BMI）］were recorded. Multivariate Logistic regression analysis was used to explore the influencing factors of CMB in patients with cerebral small vessel disease，and according to the results，a receiver operating characteristic（ROC）curve was drawn to evaluate its predictive value，and a nomogram prediction model was constructed. Results Fasting blood glucose，history of hypertension，Tyg and Tyg ⁃BMI in the CMB group were higher than those in the non⁃CMB group（P < 0.05）. Multivariate Logistic regression analysis showed that fasting blood glucose（OR = 1.388，95%CI：1.101 ~ 1.752，P = 0.006），history of hypertension（OR = 2.683，95%CI：1.041 ~ 6.916，P = 0.041），Tyg（OR = 5.808，95%CI：1.712 ~ 19.712，P = 0.005），Tyg⁃ BMI（OR = 1.034，95%CI：1.010 ~ 1.752，P=0.006）elevated were risk factors for developing CMB in patients with CSVD. A nomogram prediction model of CMB in patients with cerebral small vessel disease was established based on Tyg and Tyg⁃BMI. DCA was used for internal verification of clinical benefit，and the results suggest that the model had clinical benefit. The ROC curves of Tyg and Tyg⁃BMI for predicting the risk of CMB in patients with cerebral small vessel disease were drawn. The results showed that the area under the curve（AUC）of Tyg and Tyg⁃ BMI for predicting the risk of CMB in patients with cerebral small vessel disease were Tyg：0.700 respectively （95%CI：0.598 ~ 0.803，P < 0.01），Tyg⁃BMI：0.710（95%CI：0.618 ~ 0.802），P < 0.01）. Conclusions Insulin resistance may affect the risk of CMB in patients with cerebral small vessel disease. Insulin resistance⁃related indi⁃ cators（Tyg，Tyg⁃BMI）can have a certain predictive effect on the occurrence of CMB. Improving IR has a certain clinical value in preventing the occurrence of CMB.

Objective To investigate the positive expression of SLC7A11 in KRAS mutant pancreas and the relationship with differentiation，metastasis，staging and prognosis of pancreatic cancer. Methods 54 patients admitted to Sun Yat ⁃ sen Memorial Hospital of Sun Yat ⁃ sen University，from January 1，2019 to December 31， 2021，who were confirmed as pancreatic cancer by surgery and pathology，completed KRAS based detection，and had complete clinical data and follow⁃up conditions were selected. 27 patients showed KRAS mutation in the base detection were set as the experimental group：27 patients without KRAS mutation were set as the control group. The expression of SLC7A11 in surgical and pathological tissues was detected in all cases，and clinical data such as general condition，differentiation degree，lymph node metastasis，tumor size，disease stage，survival period were collected. The positive expression of SLC7A11 was compared between the two groups，andChi square test was used to analyze the relationship between the expression of SLC7A11 and the above factors. Results The positive expres⁃ sion rate of SLC7A11 in KRAS mutant pancreatic cancer was significantly higher than that in the control group （P = 0.002），and the positive expression of SLC7A11 was correlated with the later disease stage（P = 0.000），the worse histological grade（P = 0.003），the more lymph node metastasis（P = 0.038）and the shorter survival period （P = 0.000）. The difference was statistically significant. Conclusions The positive expression rate of SLC7A11 in pancreatic cancer tissues with KRAS mutation is high，and further detection of SLC7A11 expression should be carried out in cases with KRAS mutation. The positive expression of SLC7A11 is associated with worse disease prog⁃ nosis. SLC7A11 inhibitors are likely to be potential targeted drugs for pancreatic cancer.

Objective To compare the clinical effects of the subxiphoid approach and the intercostal approach for thoracoscopy for anterior mediastinal lesions. Methods Consecutive patients with anterior mediastinal surgery from January 2020 to May，2021 at Yichang Central People′s Hospital were retrospectively enrolled in the study. A total of 124 cases were included in the study. Sixty-three patients received the thoracoscopy via subxiphoid approach were assigned as one group and the other 61 via the intercostal approach as the other group. The clinical， pathological，and perioperative data of the two groups were collected. Statistical analyses were performed using SPSS version 22.0. Results There were no significant differences in blood loss，drainage volume，time of drainage intubation，conversion rate to thoracotomy，white blood cell value and CRP value after operation，length of hospital stay，hospitalization cost and complications between the two groups. The operation time of the subxiphoid approach group was higher than the intercostal approach group（P < 0.05）. VAS score and ratio of drainage intubation in the subxiphoid approach group were significantly lower than those in the intercostal approach group（P < 0.01）. Conclusion Both approaches for thoracoscopy are safe and effective in treating anterior mediastinal lesions. The subxiphoid approach is superior to the intercostal approach for its less damage to the intercostal nerve，less pain， no need for drainage intubation，thereby worthy of spreading clinically.

Objective To investigate the analgesic effect of ropivacaine transverse abdominal muscleplane block（TAPB）combined with patient⁃controlled intravenous analgesia（PCIA）after surgical treatment for external abdominal hernia. Methods A total of 94 patients undergoing abdominal external hernia surgery in our hospital from July 2019 to September 2021 were selected as study subjects and randomly divided into two groups. The control group（47 patients）received PCIA postoperatively，while the study group（47 patients）received additional ropivacaine TAPB analgesia on the basis of the control group. The general condition，pain scores［visual analog scale（VAS）］and sedation scores（Ramsay scale）at 2 h，6 h，12 h，24 h and 48 h after surgery，total number of PCIA compression ，total amount of infusion ，and adverse reactions were comparedbetweenthe two groups. Results There were no differences in gender，age，bodyweight，andsurgical duration between the two groups（P > 0.05）. The VAS scores increased initially and then decreased at 2 h，6 h，12 h，24 h and 48 h postoperativelyin both groups（P < 0.05）. Ramsay scores decreased gradually（P < 0.05）. VAS scores at 6 h，12 h，24 h and 48 h were lower in the study group than in the control group（P < 0.05），but there were no differences in Ramsay scores at the above time points（P > 0.05）. The total number of PCIA compression and total amount of infusion were smaller in the study group 48 h after surgery（P < 0.05）. There was no difference in the total incidence of adverse reactions between the two groups（P > 0.05）. Conclusions Ropivacaine TAPB combined with PCIA has a more significant analgesic effect than PCIA alone after surgical treatment for abdominal external hernia. This combined therapycan reduce the total number of PCIA compressionand amount of infusion volumeand it has bettersafety.

Objective To evaluate the association between tacrolimus intra⁃patient variability（IPV）and infection，rejection and death after heart transplantation. Methods Tacrolimus trough concentration（C0）was collected from heart transplant recipients in Zhengzhou Seventh People′s Hospital from April 2018 to October 2021 at 3 ~ 6 months after surgery. IPV was calculated after dose correction，and patients were divided into the high IPV group and the low IPV group. Results A total of 102 patients were enrolled，with an average IPV of 26.8%. Compared with the low IPV group，the proportion of recipients with at least one C0 outside the treatment target （8 to 12 ng/mL）was higher in the high tacrolimus IPV group（< 8 ng/mL：80.5% vs. 39.3%，χ2 = 16.836，P < 0.001；> 12 ng/mL：85.4% vs. 47.5%，χ2 = 15.007，P < 0.001）. The incidence of infection in the high IPV group was higher than that in the low IPV group（34.2% vs. 16.4%），with a 95% confidence interval of 17.8%（0.52% ~ 34.99%）. The difference was statistically significant（χ2 = 4.295，P = 0.038）. There was a statistically significant difference between the two groups in the combination of infection，rejection or death（18.03% vs. 39.02%，χ2 = 5.551，P = 0.018）. Conclusions The recipients with a high tacrolimus IPV may be more susceptible to infection after heart transplantation. Tacrolimus IPV is readily available and can be used as a follow⁃up tool to identify poor outcomes after transplantation.

Objective To explore the incidence of pain flare following radiotherapy for painful bone metas⁃ tases and evaluate the clinical value of dynamic adjustment in dose of oxycodone hydrochloride sustained ⁃ release tablets during peri⁃radiotherapeutic period in treatment of metastasis bone pain in lung cancer. Methods Clinical data of 43 lung cancer patients with metastatic bone pain in our hospital from December 2018 to September 2021 were retrospectively analyzed. Pain score，the changes of pain degrees，and corresponding dose change of oxycodone hydrochloride sustained⁃release tablets during peri⁃radiotherapeutic period were collected to analyze the feasibility and effect of dynamic adjustment of oxycodone hydrochloride sustained⁃release tablets. Results The total analge⁃ sic effective rate of combined treatment was 76.7%（33/43），and the rate of complete relief of cancer pain was 23.3%（10/43）. The incidence of single occurrence of pain flare was 20.9%，and that of two times 9.3%（4/43） during peri⁃radiotherapeutic period. The incidence rate of gradual reduction of oxycodone hydrochloride sustained⁃ release tablets dose during peri⁃radiotherapeutic period was 86.04%（37/43），and that of gradual reduction of oxy⁃ codone hydrochloride sustained ⁃ release tablets dose 74.41%（32/43）within 1 month after radiotherapy. Dynamic adjustment in dose of tablets was required during the period. Conclusion Radiotherapy combined with opioids is a well ⁃ established and effective treatment alternative for symptomatic bone metastases. Pain flare is a common event，occurring in 20.9% of the patients receiving radiotherapy for symptomatic bone metastases. Dynamic adjust⁃ ment in dose of oxycodone hydrochloride sustained ⁃ release tablets could improve the efficacy and avoid opioid abuse.

Objective To conduct a panoramic survey and analysis of the current scientific research situa⁃ tion of major Anesthesiology Hospitals in Guangdong Province，so as to provide reference for the scientific research and construction of the anesthesia team in the province. Methods A self⁃designed questionnaire was used to ana⁃ lyze the anesthesiology related scientific research in 35 major hospitals of anesthesiology in Guangdong Province by online survey. The survey was conducted from October to December 2020. Results A total of 1 788 valid question⁃ naires were obtained，with a gender ratio of about 8∶3 and an average age of 37 years（18 ~ 60 years）. According to the analysis of human resources，321 doctoral students（17.95%），853 graduate students（47.71%），503 un⁃ dergraduate students（28.13%）and 111 junior college students（6.21%）were included. The analysis of scientific research funds showed that 829 total projects including 179 national funds，371 provincial funds，279 municipal funds were obtained from 2011 to 2020，and 493 projects including 114 national funds，205 provincial funds，161 municipal funds were obtained from 2016 to 2020. According to the analysis of papers published，1 116 sci⁃indexed papers and 1 441 national journals were published in 2011-2020，795 sci⁃indexed papers and 914 national jour⁃ nals were published in 2016-2020. The analysis of awards showed that 8 national awards，53 provincial funds and 38 municipal funds were won in 2011-2020，5 national awards，46 provincial funds and 24 municipal funds were won in 2016-2020. The analysis of patents and publications showed that 334 patents and 108 publications were obtained in 2011-2020，232 patents and 82 publications were obtained in 2016-2020. Conclusion The talent team of anesthesiology in Guangdong Province is growing rapidly，and the development trend of scientific research is fast. All units in Guangdong Province should attach importance to and issue better scientific research related poli⁃cies，and strive to promote anesthesiology of Guangdong to form a more mature frontier research direction，signifi⁃ cantly improve the comprehensive strength and innovation ability of the discipline，and better adapt to the needs of anesthesiology technical talents in the new era.

Pain，nausea and vomiting，and other adverse events after laparoscopic surgery seriously affect the recovery of patients. Therefore，seeking better analgesia and effectively reducing postoperative complications are important goals in enhance recovery after surgery. As a long⁃term amide local anesthetic，ropivacaine can not only block nerve excitation and conduction，but also has the effects of anti⁃inflammation，inhibition of cell injury and anti⁃cancer. Intraperitoneal administration of ropivacaine can reduce postoperative pain and surgical stress lev⁃ els；reduce the incidence of nausea and vomiting，and promote gastrointestinal function recovery. It is conducive to accelerating postoperative rehabilitation. This review summarizes the clinical effects，possible mechanisms，safety， and administration strategies of intraperitoneal administration of ropivacaine.

Drug ⁃induced liver injury（DILI）is one of the most common and serious adverse drug reac⁃ tions，which is difficult to diagnose clinically due to the lack of specific markers. Many studies have reported that miR⁃122 is a potential biomarker of hepatotoxicity. Therefore，this paper describes the temporal changes of miR⁃ 122 expression in DILI，the correlation between miR ⁃122 and the liver regeneration and repair process of DILI， and the correspondence between miR⁃122 expression level and the degree of liver histopathological damage，aiming to summarize the dynamic expression pattern of miR⁃122.