蟛蜞菊内酯对肺炎链球菌感染的肺泡上皮细胞凋亡及炎症因子分泌的调节作用

doi:10.3969/j.issn.1006-5725.2024.03.006

Abstract

Abstract:

Objective To investigate the regulatory effects of wedelobata on apoptosis and secretion of inflammatory factors in the alveolar epithelial cells infected by Streptococcus pneumoniae. Methods Alveolar epithelial cells A549 were divided into infection group （1 × 10⁸ /CFU/mL cultured cells of Streptococcus pneumoniae）， control group （no treatment）， infection+wedelolactone low-dose group， middle-dose group and high-dose group （pretreated with wedelolactone at 10， 20 and 40 μmol/L and then cultured cells of Streptococcus pneumoniae at 1 × 10⁸ /CFU/mL）. Alveolar epithelial cells A549 were divided into infection group （1 × 10⁸ /CFU/mL cultured cells of Streptococcus pneumoniae）， control group （no treatment）， infection+wedelolactone low-dose group， middle-dose group and high-dose group （pretreated with wedelolactone at 10， 20 and 40 μmol/L and then cultured cells of Streptococcus pneumoniae at 1 × 10⁸ /CFU/mL）. Results Compared with control group， the apoptosis rate， the relative expression levels of Bax， Caspase-3 protein， IL-6， IL-1β and TNF-α mRNA were higher in infection group， infection + wedelolide low dose group， medium dose group and high dose group， while the expression level of Bcl-2 protein was lower （P < 0.05）. Compared with the infected group， the apoptosis rate， the relative expression levels of Bax， Caspase-3 protein， IL-6， IL-1β and TNF-α mRNA and the expression levels of Bcl-2 protein were lower in the infected + wedelolide low dose， medium dose and high dose groups. Moreover， the apoptosis rate， the expression levels of Bax， Caspase-3 protein， IL-6， IL-1β and TNF-α mRNA were the highest in the infected + wedelactone high-dose group， and the expression levels of Bcl-2 protein were the lowest （P < 0.05）. Conclusion The apoptosis rate of alveolar epithelial cells infected by Streptococcus pneumoniae decreased and the secretion of inflammatory factors decreased after the intervention of wedelia lactone.

Key words: wedelia chinensis lactone, streptococcus pneumoniae, alveolar epithelial cells, apoptosis, inflammatory factor

CLC Number:

R563

Lihong DING,Shijia GENG,Yujie WANG. Effects of wedelobata on apoptosis and secretion of inflammatory cytokines in the alveolar epithelium infected by Streptococcus pneumonia[J]. The Journal of Practical Medicine, 2024, 40(3): 316-320.

Figures/Tables 6

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References 22

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组别	凋亡率
对照组	5.86 ± 0.42
感染组	32.38 ± 2.90^a
感染+蟛蜞菊内酯低剂量组	23.96 ± 2.45^ab
感染+蟛蜞菊内酯中剂量组	19.27 ± 2.06^abc
感染+蟛蜞菊内酯高剂量组	10.37 ± 1.10^abcd
F值	11.490
P值	0.001

组别	Bax	Bcl-2	Caspase-3
对照组	0.29 ± 0.04	0.82 ± 0.11	0.21 ± 0.03
感染组	0.76 ± 0.12^a	0.30 ± 0.06^a	0.82 ± 0.14^a
感染+蟛蜞菊内酯低剂量组	0.68 ± 0.09^ab	0.41 ± 0.07^ab	0.71 ± 0.11^ab
感染+蟛蜞菊内酯中剂量组	0.60 ± 0.06^abc	0.52 ± 0.09^abc	0.58 ± 0.06^abc
感染+蟛蜞菊内酯高剂量组	0.38 ± 0.05^abcd	0.70 ± 0.10^abcd	0.40 ± 0.05^abcd
F值	4.217	2.422	9.775
P值	0.001	0.028	0.001

组别	IL-6	IL-1β	TNF-α
对照组	1.00 ± 0.01	1.00 ± 0.01	1.00 ± 0.01
感染组	2.13 ± 0.23^a	2.20 ± 0.30^a	2.25 ± 0.31^a
感染+蟛蜞菊内酯低剂量组	1.80 ± 0.19^ab	1.76 ± 0.20^ab	1.78 ± 0.21^ab
感染+蟛蜞菊内酯中剂量组	1.65 ± 0.15^abc	1.63 ± 0.18^abc	1.66 ± 0.19^abc
感染+蟛蜞菊内酯高剂量组	1.23 ± 0.11^abcd	1.25 ± 0.12^abcd	1.30 ± 0.14^abcd
F值	6.247	6.228	6.412
P值	0.001	0.001	0.001

组别	IL-6（pg/mL）	IL-1β（pg/mL）	TNF-α（ng/mL）
对照组	81.26 ± 9.45	21.39 ± 3.06	9.12 ± 1.03
感染组	368.45 ± 50.21^a	80.46 ± 10.03^a	59.06 ± 10.37^a
感染+蟛蜞菊内酯低剂量组	300.41 ± 42.27^ab	62.89 ± 7.26^ab	50.31 ± 8.06^ab
感染+蟛蜞菊内酯中剂量组	221.39 ± 36.24^abc	50.30 ± 5.90^abc	41.56 ± 7.24^abc
感染+蟛蜞菊内酯高剂量组	154.37 ± 21.76^abcd	40.27 ± 4.96^abcd	23.16 ± 3.45^abcd
F值	9.245	9.719	11.700
P值	0.001	0.001	0.001

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Effects of wedelobata on apoptosis and secretion of inflammatory cytokines in the alveolar epithelium infected by Streptococcus pneumonia

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