miRNAs调节的免疫炎性机制在脊髓损伤致骨质疏松症患者发病中的调控机制

doi:10.3969/j.issn.1006-5725.2023.19.012

Abstract

Abstract:

Objective To explore the regulatory role of immunoinflammatory mechanism regulated by tiny RNAs （miRNAs） in the pathogenesis of spinal cord injury-induced osteoporosis （CSCI-OP）. Methods Thirty patients with CSCI-OP admitted to our hospital from July 2021 to July 2022 were selected as the observation group， fifty patients with senile osteoporosis were selected as the control group， and fifty healthy people were selected as the normal control group. The three groups were compared in terms of hormone indexes， inflammatory factors， miRNAs expression and bioinformatics analysis. The main factors affecting the pathogenesis of CSCI-OP patients were detected. Results Compared with the spinal cord injury group， the T and TNF-α levels were increased， and the E2， IL-6 and BLAP levels were decreased in the senile osteoporosis group （P < 0.05）， but there was no significant difference in 25-OHD and PTH levels between the two groups （P > 0.05）. Univariate analysis showed that the incidence of CSCI-OP was mainly related to age， sex， duration of spinal cord injury and diabetes mellitus （P < 0.05）. Compared with the normal control group， the expression of IL-6 miRNA and BLAP miRNA in the control group and observation group was increased and the expression of TNF-α miRNA was decreased （P < 0.05）. Conclusion MiRNAs can be effectively regulated in CSCI-OP. It can significantly slow down the occurrence and development of CSCI-OP through targeted immune inflammatory mechanisms.

Key words: micro-RNAs, immune inflammatory mechanism, spinal cord injury, osteoporosis

CLC Number:

R361

Cong CHEN,Huaijie XING,Min CHEN,Chaosheng ZENG,Qingjie. SU. Regulatory role of miRNAs in the pathogenesis of osteoporosis induced by spinal cord injury[J]. The Journal of Practical Medicine, 2023, 39(19): 2483-2488.

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References 24

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组别	例数	E₂（pmol/L）	T（ng/mL）	25-OHD（ng/mL）	PTH（pg/mL）
脊髓损伤组	30	145.95 ± 34.02	8.72 ± 2.13	14.22 ± 4.26	65.28 ± 16.57
老年骨质疏松组	50	128.56 ± 20.88	14.23 ± 3.56	11.72 ± 5.07	63.44 ± 23.06
t值		14.077	8.246	2.668	1.935
P值		0.007	0.012	0.546	0.207

组别	例数	IL-6（U/L）		TNF-α（ng/mL）		BLAP（ng/mL）
组别	例数	男	女	男	女	男	女
脊髓损伤组	30	174.61 ± 24.34	152.55 ± 18.29	30.12 ± 4.31	34.61 ± 5.83	198.46 ± 26.04	195.88 ± 32.83
老年骨质疏松组	50	123.02 ± 19.83	125.55 ± 5.04	37.22 ± 6.16	40.16 ± 6.71	164.24 ± 41.02	151.83 ± 31.05
t值		10.023	6.104	4.615	3.757	8.521	12.233
P值		0.001	0.008	0.021	0.001	0.012	0.001

因素	例数	T值＞-2.5（n = 16）	T值≤-2.5（n = 14）	χ ²/t值	P值
年龄
20 ~ 30岁	6	5（83.30）	1（16.70）	9.100	0.001
31 ~ 40岁	24	11（45.80）	13（54.20）	9.100	0.001
性别
男	18	12（66.70）	6（33.30）	6.233	0.018
女	12	4（33.30）	8（66.70）	6.233	0.018
BMI
< 25 kg/m²	22	14（63.80）	8（36.40）	4.667	0.035
≥ 25 kg/m²	8	2（25.00）	6（75.00）	4.667	0.035
饮酒史
是	18	9（50.00）	9（50.00）	1.233	0.514
否	12	7（59.40）	5（41.60）	1.233	0.514
高血压
是	6	4（66.70）	2（33.30）	2.199	0.203
否	24	12（50.00）	12（50.00）	2.199	0.203
高脂血症
是	4	3（75.00）	1（25.00）	3.106	0.087
否	26	13（50.00）	13（50.00）	3.106	0.087
脊髓损伤病程
< 1个月	6	5（83.30）	1（16.70）	8.537	0.008
1 ~ 5个月	18	8（44.40）	10（55.60）
> 5个月	6	3（50.00）	3（50.00）
糖尿病
是	1	1（100.00）	0（0.00）	4.533	0.041
否	29	15（51.70）	14（48.30）	4.533	0.041
吸烟史
有	16	8（50.00）	8（50.00）	1.055	0.688
无	14	8（57.10）	6（42.90）	1.055	0.688

组别	IL-6 miRNA	TNF-α miRNA	BLAP miRNA
NC组	1.00 ± 0.01	1.00 ± 0.01	1.00 ± 0.01
OP组	0.67 ± 0.08^a	0.34 ± 0.04^a	0.70 ± 0.10^a
NCmimic+OP组	0.40 ± 0.05^ab	0.65 ± 0.09^ab	0.38 ± 0.06^ab
F值	83.210	27.330	72.070
P值	0.001	0.001	0.001

组别	IL-6 miRNA		TNF-α miRNA		BLAP miRNA
组别	WT	MUT	WT	MUT	WT	MUT
miR-NC组	1.19 ± 0.13	1.02 ± 0.09	1.15 ± 0.11	1.01 ± 0.08	1.17 ± 0.13	1.01 ± 0.10
miRNAs组	0.42 ± 0.10	0.99 ± 0.07	0.47 ± 0.08	0.98 ± 0.07	0.45 ± 0.12	0.99 ± 0.09
t值	41.760	1.762	35.350	1.330	28.780	1.577
P值	0.001	0.081	0.001	0.186	0.001	0.118

Regulatory role of miRNAs in the pathogenesis of osteoporosis induced by spinal cord injury

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